Primers and Thermal Cycling Protocol
DNA EXTRACTION
See Technical Information section
PCR MIX (50 µl)
Buffer 10X, 3 mM MgCl2 (check if in your buffer there is MgCl2, in that case you have to adjust the
magnesium quantity to reach a final concentration of 3 mM), 400µM dNTPs, 20 pmol di each primer, 2.5 U di Taq polimerasi, 400 ng DNA sample.
Primers (usually I make a stock solution of 5 pmol/µl and then I put 4 µl of each primer in the mix):
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