4MRNA can run in any environment that supports shell scripts and the external software packages 3DNA and Phaser. It has been tested on the following operating systems:

• Windows (via Windows Subsystem for Linux, Ubuntu)

• macOS

• Linux (Rocky Linux)

4MRNA itself is distributed under the MIT License. This means that it can be used completely freely, provided that the copyright notice is included.

However, please pay attention to the licenses of the dependencies. 4MRNA relies on several external resources, including 3DNA, Phenix, and CCP4/Phaser, but these components are not included in the distribution of 4MRNA. Users are responsible for installing them separately and complying with their respective licenses. A summary of these dependencies is provided in "DEPENDENCIES.txt" on GitHub.

If you use 4MRNA in your research, please cite the following reference. In addition, please also cite the external software packages used by 4MRNA.

• 4MRNA: The manuscript describing 4MRNA is currently in preparation. Until it becomes available, please cite the following GitHub repository: https://github.com/S-Ando-Biophysics/4MRNA.

• 3DNA: Lu, X. J., & Olson, W. K. (2008). 3DNA: a versatile, integrated software system for the analysis, rebuilding and visualization of three-dimensional nucleic-acid structures. Nature protocols, 3(7), 1213–1227. https://doi.org/10.1038/nprot.2008.104 

• Phenix: Liebschner, D., Afonine, P. V., Baker, M. L., Bunkóczi, G., Chen, V. B., Croll, T. I., Hintze, B., Hung, L. W., Jain, S., McCoy, A. J., Moriarty, N. W., Oeffner, R. D., Poon, B. K., Prisant, M. G., Read, R. J., Richardson, J. S., Richardson, D. C., Sammito, M. D., Sobolev, O. V., Stockwell, D. H., … Adams, P. D. (2019). Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix. Acta crystallographica. Section D, Structural biology, 75(Pt 10), 861–877. https://doi.org/10.1107/S2059798319011471 

• Phaser: McCoy, A. J., Grosse-Kunstleve, R. W., Adams, P. D., Winn, M. D., Storoni, L. C., & Read, R. J. (2007). Phaser crystallographic software. Journal of applied crystallography, 40(Pt 4), 658–674. https://doi.org/10.1107/S0021889807021206 

If you find a bug, please let me know using this form.