4MRNA itself is distributed under the MIT License. This means that it can be used completely freely, provided that the copyright notice is included.

However, please pay attention to the licenses of the dependencies. 4MRNA relies on several external resources, including 3DNA, Phenix, and CCP4/Phaser, but these components are not included in the distribution of 4MRNA. Users are responsible for installing them separately and complying with their respective licenses. A summary of these dependencies is provided in "DEPENDENCIES.txt" on GitHub.

4MRNA can run in any environment that supports shell scripts and the external software packages 3DNA and Phaser. It has been tested on the following operating systems:

• Windows (via Windows Subsystem for Linux, Ubuntu)

• macOS

• Linux (Rocky Linux)