Please run the following commands in order. 

cd ~

git clone https://github.com/S-Ando-Biophysics/4MRNA-Install.git

cd 4MRNA-Install

chmod +x install.sh

./install.sh

echo 'export PATH="$HOME/4MRNA-Install/bin:$PATH"' >> ~/.bashrc

source ~/.bashrc

In addition, please install and set up the following external software in advance.

• Ubuntu: https://apps.microsoft.com/search?query=Ubuntu 

• 3DNA: http://forum.x3dna.org/site-announcements/download-instructions/ 

• Phenix: https://phenix-online.org/download 

• Phaser: https://www.ccp4.ac.uk/download 

This is required only for Windows. It is necessary to turn on "Windows Subsystem for Linux (WSL)" and "Virtual Machine Platform" in the Windows settings to be able to use shell scripts. Furthermore, run  the following commands.

sudo apt update

sudo apt upgrade

sudo apt install -y bzip2 curl git wget

sudo apt install libgomp1 libstdc++6 libgcc-s1 libc6

sudo ln -s /usr/bin/python3 /usr/bin/python

After registering on the official website (forum) and receiving approval, you will be able to download the installer. For details, please refer to the instructions on the official website. Once you have downloaded the installer, run the following commands in order. The following steps are for Windows (WSL, Ubuntu). The procedure for macOS and Linux is similar.

# Please change the directory name and 3DNA version as appropriate.

# Assume that "x3dna-v2.4-linux-64bit.tar.gz" has been downloaded to "C:\Users\name\Downloads".

sudo apt update

sudo apt install ruby

sudo su

cd /usr/local

mv /mnt/c/Users/name/Downloads/x3dna-v2.4-linux-64bit.tar.gz .

tar pzxvf x3dna-v2.4-linux-64bit.tar.gz

cd x3dna-v2.4/bin

./x3dna_setup

exit

echo 'export X3DNA=/usr/local/x3dna-v2.4' >> ~/.bashrc

echo 'export PATH="$X3DNA/bin:$PATH"' >> ~/.bashrc

source ~/.bashrc

Please download the command-line installer from the official website. Then please run the following commands in order. The following steps are for Windows (WSL, Ubuntu). The procedure for macOS and Linux is similar.

# Please change the directory name and Phenix version as appropriate.

# Assume that "Phenix-2.0-5936-Linux-x86_64.sh" has been downloaded to "C:\Users\name\Downloads".

sudo su

cd /usr/local

mv /mnt/c/Users/name/Downloads/Phenix-2.0-5936-Linux-x86_64.sh .

bash Phenix-2.0-5936-Linux-x86_64.sh -b -p /usr/local/phenix-2.0-5936

exit

echo "source /usr/local/phenix-2.0-5936/phenix_env.sh" >> ~/.bashrc

source ~/.bashrc

Phaser is included in the CCP4 suite, so please download the CCP4 installer from the official website. After that, run the following commands in order. The following steps are for Windows (WSL, Ubuntu). The procedure for macOS and Linux is similar.

# Please change the directory name and CCP4 version as appropriate.

# Assume that "ccp4-9.0.010-linux64.tar.gz" has been downloaded to "C:\Users\name\Downloads".

sudo su

cd /usr/local

mv /mnt/c/Users/name/Downloads/ccp4-9.0.010-linux64.tar.gz .

gunzip ccp4-9.0.010-linux64.tar.gz

tar -xvf ccp4-9.0.010-linux64.tar

cd ccp4-9

./BINARY.setup

exit

echo "source /usr/local/ccp4-9/bin/ccp4.setup-sh" >> ~/.bashrc

source ~/.bashrc