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Basic Methods in Protein Purification and Analysis: A Laboratory Manual PDF

by : Richard J. Simpson

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Book Description:

Understanding how proteins function is an essential part of many biological research endeavors. The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. Thus, reducing the complexity ofa protein sample or in some cases purifying a protein to homogeneity is necessary. The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing proteinDSprotein interactions.The protocols are supported by background information to assist researchers in understanding how the purification methods work and how to optimize and troubleshoot the methods. The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from thepopular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and ProteinDSProtein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts--acritical and often neglected step in successful protein purification. Rounding out the manual are methods for characterizing proteinDSprotein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, andimmunoblotting. Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases.

Book Detail:

Title: Basic Methods in Protein Purification and Analysis: A Laboratory Manual

Author : Richard J. Simpson

Pages : 436

Publisher : Cold Spring Harbor Laboratory Press

Language :

ISBN : 0879698675