Choose the number of crystallization conditions, e.g. 100 for a 96-well crystallization plate
Choose the total volume of the crystallization droplet, e.g. 0.5 μl
Choose whether you perform seeding or not
The percentage of the seeding solution, e.g. 5%
The percentage of the protein solution in the crystallization droplet, typically between 30-70%, most commonly 50%
Concentration of the protein solution in mg/ml
Protein MW in Daltons
Ligand stock solution concentration in mM
The molecular excess of the ligand over the protein in the droplets, e.g. for high affinity ligands 2-10, while for low >50. Highly dependent on DMSO percentage in droplets
Choose whether the ligand is in DMSO or not