(32) Pantouris, G.*; Khurana, L.; Tilstam, P.; Benner, A.; Cho, T.Y.; Lelaidier, M.; Perrée, M.; Rosenbaum, Z.; Leng, L.; Foss, F.; Bhandari, V.; Verma, A.; Bucala, R.; Lolis, E.J.*, Inhibition of MIF with an Allosteric Inhibitor Triggers Cell Cycle Arrest in Acute Myeloid Leukemia, ACS Omega 2025.

https://pubs.acs.org/action/doSearch?SeriesKey=acsodf&AllField=Pantouris 

(31) Argueta, C.; Parkins, A.; Pantouris, G.*, Conformational Flexibility of the C-Terminal Region Influences Distal Active Site Residues Across the Tautomerase Superfamily, Int J Mol Sci. 2024, 25(23):12617.

• PMID: 39684328

• DOI: 10.3390/ijms252312617

(30) Li, J.; Leng, L.; Pantouris, G.; Manjula, R.; Piecychna, M.; Abriola, L.; Hu, B.; Lolis, E.; Armstrong, M.E.; Donnelly, S.C.; Bucala, R.*, A small-molecule allele-selective transcriptional inhibitor of the MIF immune susceptibility locus, J Biol Chem. 2024, 300(7):107443. 

• PMID: 38838773

• DOI: 10.1016/j.jbc.2024.107443

(29) Parkins, A.; Pilien, A.V.R.; Wolff, A.M.; Argueta, C.; Vargas, J.; Sadeghi, S.; Franz, A.H.; Thompson, M.C., Pantouris, G.*, The C-terminal Region of D-DT Regulates Molecular Recognition for Protein-Ligand Complexes, J Med Chem. 2024, 67(9):7359-7372.

• PMID: 38670943

• DOI: 10.1021/acs.jmedchem.4c00177

(28) Bloom, J*; Pantouris, G.*; He, M.; Aljabari, B.; Mishra, L.; Manjula, R.; Parkins, A.; Lolis, E.J.*; Al-Abed, Y.*, Iguratimod, an allosteric inhibitor of macrophage migration inhibitory factor (MIF), prevents mortality and oxidative stress in a murine model of acetaminophen overdose, Mol Med. 2024, 30(1):43.

• PMID: 38539088 

• DOI: 10.1186/s10020-024-00803-0

(27) Vargas, J.; Pantouris G.*, Analysis of CD74 Occurrence in Oncogenic Fusion Proteins, Int. J. Mol. Sci. 2023, 24(21), 15981.

• PMID: 37958963

• DOI: 10.3390/ijms242115981

(26) Parkins, A.; Pantouris G.*, Protocol for purification and enzymatic characterization of members of the human macrophage migration inhibitory factor superfamily, STAR Protoc. 2023, 4(3):102375.

• PMID: 37355993

•  DOI: 10.1016/j.xpro.2023.102375

(25) Argueta, C; Vargas, J.; Parkins, A.; Ren, J.; Pantouris G.*, Hands-On Methods to Educate High School Students about Cancer Research, J. Chem. Educ. 2023, 100, 6, 2312–2319.

• https://doi.org/10.1021/acs.jchemed.3c00141 

(24) Parkins, A; Pantouris G.*, A novel enzymatic assay for the identification of 4-hydroxyphenylpyruvate dioxygenase modulators, STAR Protoc. 2023, 4(2):102300.

• PMID:37167058

• DOI: 10.1016/j.xpro.2023.102300

(23) Parkins, A; Sandin, SI; Knittel, J; Franz, AH; Ren, J; de Alba, E; Pantouris G.*, Underrepresented Impurities in 4-Hydroxyphenylpyruvate Affect the Catalytic Activity of Multiple Enzymes, Anal Chem 2023, 95(11):4957-4965.

• PMID:36877482

• DOI: 10.1021/acs.analchem.2c04969

(22) Parkins, A; Chen, E; Rangel, VM; Singh, M; Xue, L; Lisi, GP; Pantouris G.*, Ligand-induced conformational changes enable intersubunit communications in D-dopachrome tautomerase, Biophys J. 2023, 122(7):1268-1276.

• PMID:36804669

• DOI: 10.1016/j.bpj.2023.02.019

(21) Parkins, A.; Das, P.; Prahaladan, V.; Rangel, VM.; Xue, L.; Sankaran, B.; Bhandari, V.; Pantouris G.*, 2,5-Pyridinedicarboxylic acid is a bioactive and highly selective inhibitor of D-dopachrome tautomerase, Structure 2023, 2;31(3):355-367.

• PMID: 36805127

• DOI: 10.1016/j.str.2023.01.008

(20) Skeens, E.; Pantouris, G.; Shah, D.; Manjula, R.; Ombrello, M. J.; Maluf, N. K.; Bhandari, V.; Lisi, G. P.; Lolis, E. J., A Cysteine Variant at an Allosteric . Site Alters MIF Dynamics and Biological Function in Homo- and Heterotrimeric Assemblies, Front Mol Biosci  2022, 9, 783669.

• PMID: 35252348

• DOI: 10.3389/fmolb.2022.783669

(19) Tilstam, P. V.; Schulte, W.; Holowka, T.; Kim, B. S.; Nouws, J.; Sauler, M.; Piecychna, M.; Pantouris, G.; Lolis, E.; Leng, L.; Bernhagen, J.; Fingerle-Rowson, G.; Bucala, R. , MIF but not MIF-2 recruits inflammatory macrophages in an experimental polymicrobial sepsis model, J Clin Invest 2021, 131 (23):e127171.

• PMID: 34850744

• DOI: 10.1172/JCI127171

(18) Parkins, A.; Skeens, E.; McCallum, C. M.; Lisi, G. P.; Pantouris, G.* The N-terminus of MIF regulates the dynamic profile of residues involved in CD74 activation, Biophys J  2021, 120, 3893-3900. 

• PMID: 34437846

• DOI: 10.1016/j.bpj.2021.08.025

(17) Rosenberg, E. M., Jr.; Herrington, J.; Rajasekaran, D.; Murphy, J. W.; Pantouris, G.; and Lolis, E. J., The N-terminal length and side-chain composition of CXCL13 affect crystallization, structure and functional activity. Acta Crystallogr D Struct Biol  2020, 76, 1033-1049.

• PMID: 33021505

• DOI: 10.1107/S2059798320011687 

(16) Gannam, Z.T.K; Min, K.; Shillingford, S.R.; Zhang, L.; Herrington, J.; Abriola, L.; Gareiss, P.C.; Pantouris, G.; Tzouvelekis, T.; Kaminski, N.; Zhang, X.; Yu, J.; Jamali, H.; Ellman, J.A.; Lolis, E.; Anderson, K.S.; Bennett, A.M, An allosteric site on MKP5 reveals a strategy for small-molecule inhibition, Sci. Signal. 2020, 13(646).

• PMID: 32843541

• DOI: 10.1126/scisignal.aba3043 

(15) Pantouris, G.;* Khurana, L.; Ma, A.; Skeens, E.; Reiss, K.; Batista, V.S.; Lisi,* G.P.; Lolis,* E.J., Regulation of MIF enzymatic activity by an allosteric site at the central solvent channel, Cell Chem. Biol. 2020, 27(6):740-750.

• PMID: 32433911

• DOI: 10.1016/j.chembiol.2020.05.001 

(14) Tilstam, P.V.; Pantouris, G.; Corman, M.; Andreoli, M.; Mahboubi, K.; Davis, G.; Du, X.; Leng, L.; Lolis, E.; Bucala, R.,* A selective small-molecule inhibitor of macrophage migration inhibitory factor-2 (MIF-2), a MIF cytokine superfamily member, inhibits MIF-2 biological activity, J. Biol. Chem. 2019, 294(49):18522-18531. 

• PMID: 31578280

• DOI: 10.1074/jbc.RA119.009860

(13) Khurana, L.; ElGindi, M.; Tilstam, P.V.; Pantouris, G.*, Elucidating the role of an immunomodulatory protein in cancer: From protein expression to functional characterization, Methods Enzymol. 2019, 629: 307-360.

• PMID: 31727247

• DOI: 10.1016/bs.mie.2019.05.053

(12) Pantouris, G.; Dioletis E.; Chen Y.; Thompson D.C.; Vasiliou V.;* Lolis E.J.,* Expression, Purification and Crystallization of the Novel Xenopus tropicalis ALDH16B1, a Homologue of Human ALDH16A1, Chem Biol Interact. 2019,1;304:168-172. 

• PMID: 30894314

• DOI: 10.1016/j.cbi.2019.03.009

(11) Pantouris, G.; Bucala R.; Lolis E.J.,* Structural Plasticity at the C-terminal Region of Macrophage Migration Inhibitory Factor-2 Is Associated with an Induced Fit Mechanism for a Selective inhibitor, Biochemistry 2018, 57(26):3599-3605. 

• PMID: 29847104

• DOI: 10.1021/acs.biochem.8b00344

(10) Pantouris, G.; Ho J.; Shah D.; Syed M.S.; Leng L.; Bhandari V.; Bucala R.; Batista, V.S.; Loria J.P.;* Lolis E.J.,* Nanosecond Dynamics Regulate the MIF-Induced Activity of CD74, Angew. Chem. Int. Ed. 2018, 57, 7116-7119. 

• PMID: 29669180

• DOI: 10.1002/anie.201803191

(9) Lacy, M.; Kontos, C.; Brandhofer, M.; Hille, K.; Gröning, S.; Sinitski, D.; Bourilhon, P.; Rosenberg, E.; Krammer, C.; Thavayogarajah, T.; Pantouris, G.; Bakou, M.; Weber, C.; Lolis, E.; Bernhagen, J.;* Kapurniotu, A.,* Identification of an Arg-Leu-Arg tripeptide that contributes to the binding interface between the cytokine MIF and the chemokine receptor CXCR4, Sci. Rep. 2018, 8 (1), 5171. 

• PMID: 29581527

• DOI: 10.1038/s41598-018-23554-5

(8) Singh, A. K.; Pantouris, G.; Borosch, S.; Rojanasthien, S.; Cho, T. Y.,* Structural basis for decreased induction of class IB PI3-kinases expression by MIF inhibitors, J Cell Mol Med 2017, 21(1),142-153.

• PMID: 27619729

• DOI: 10.1111/jcmm.12949

(7) Meza-Romero, R.; Benedek, G.; Jordan, K.; Leng, L.; Pantouris, G.; Lolis, E.; Bucala, R.; Vandenbark, A. A.,* Modeling of both shared and distinct interactions between MIF and its homologue D-DT with their common receptor CD74, Cytokine 2016, 88, 62-70.

• PMID: 27573366

• DOI: 10.1016/j.cyto.2016.08.024

(6) Pantouris, G.*; Loudon-Griffiths, J.; Mowat, C. G.,* Insights into the mechanism of inhibition of tryptophan 2,3-dioxygenase by isatin derivatives, J Enzyme Inhib Med Chem 2016, 1-9.

• PMID: 27096472

• DOI: 10.3109/14756366.2016.1170013

(5) Stoppe, C.;* Rex, S.; Goetzenich, A.; Kraemer, S.; Emontzpohl, C.; Soppert, J.; Averdunk, L.; Sun, Y.; Rossaint, R.; Lue, H.; Huang, C.; Song, Y.; Pantouris, G.; Lolis, E.; Leng, L.; Schulte, W.; Bucala, R.; Weber, C.; Bernhagen, J.,* Interaction of MIF Family Proteins in Myocardial Ischemia/Reperfusion Damage and Their Influence on Clinical Outcome of Cardiac Surgery Patients, Antioxid Redox Signal 2015, 23 (11), 865-79.

• PMID: 26234719

• DOI: 10.1089/ars.2014.6243

(4) Pantouris, G.; Syed, M. A.; Fan, C.; Rajasekaran, D.; Cho, T. Y.; Rosenberg, E. M., Jr.; Bucala, R.; Bhandari, V.; Lolis, E. J.,* An Analysis of MIF Structural Features that Control Functional Activation of CD74, Chem Biol 2015, 22 (9), 1197-205.

• PMID: 26364929

• DOI: 10.1016/j.chembiol.2015.08.006

(3) Pantouris, G.; Rajasekaran, D.; Garcia, A. B.; Ruiz, V. G.; Leng, L.; Jorgensen, W. L.; Bucala, R.; Lolis, E. J.,* Crystallographic and receptor binding characterization of Plasmodium falciparum macrophage migration inhibitory factor complexed to two potent inhibitors, J. Med. Chem. 2014, 57 (20), 8652-6.

• PMID: 25268646

• DOI: 10.1021/jm501168q

(2) Pantouris, G.; Serys, M.; Yuasa, H. J.; Ball, H. J.; Mowat, C. G.,* Human indoleamine 2,3-dioxygenase-2 has substrate specificity and inhibition characteristics distinct from those of indoleamine 2,3-dioxygenase-1, Amino acids 2014, 46 (9), 2155-63.

• PMID: 24875753

• DOI: 10.1007/s00726-014-1766-3

(1) Pantouris, G.; Mowat, C. G.,* Antitumour agents as inhibitors of tryptophan 2,3-dioxygenase, Biochem. Biophys. Res. Commun. 2014, 443 (1), 28-31. 

• PMID: 24269239

• DOI: 10.1016/j.bbrc.2013.11.037