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Somatic cilia (ci), oral apparatus (oa), cytopharyngeal microtubules (cm), postoral fiber (pof), cytoproct (cp) and radial arms microtubules of the contractile vacuole complexes (cvc) were labeled with mAb N356 (Green). While, the macronucleus (ma) and micronuclei (mi) were labeled with DAPI (Red: computer generated pseudocolor). The picture showing below is a fluorescence image of P. caudatum stained with mAb N356.
This GIF image was taken by the conforcal laser scanning microscope (Fluoview FV10i, Olympus), where P. multimicronucleatum was stained by indirect immuno-fluorescence with mAb N356 (Amersham) and DAPI (SIGMA). Related paper information : https://doi.org/10.2108/zsj.16.915
Schematic diagram of Paramecium caudatum created by PowerPoint. The name of each part of the cell is shown.
Localization of GFP-tagged AQP1 on contractile vacuole complex in P. multimicronucleatum. Cell was fixed and incubated with DS-1, a monoclonal antibody against A4 antigen of the V-ATPase (Allen et al., 1990; Fok et al., 1995; Fok et al., 2002), allowing visualization of decorated spongiome in red and the AQP1 in green. Related paper information:https://doi.org/10.1111/jeu.12843
This GIF image shows the Localization of GFP-tagged AQP1 on contractile vacuole complex in P. multimicro-nucleatum. Cell was fixed and incubated with DS-1, a monoclonal antibody against A4 antigen of the V-ATPase (Allen et al., 1990; Fok et al., 1995; Fok et al., 2002), allowing visualization of decorated spongiome in red and the AQP1 in green.
Schematic diagram of the contractile vacuole complex created. AMP, ampullae; AQP, aquaporin; CC, collecting canal; CV, contractile vacuole; CVP, pore; DS, decorated spongiome; MTR, microtubular ribbon; SS, smooth spongiome. Related paper information: https:// doi.org/10.1111/jeu.12843
Single contractile vacuole complex (CVC) observed in side view (upper case) and overhead view (lower case) undergoing cycles of filling (diastole) and expulsion (systole) of its fluid content as the contractile vacuole (CV) membrane fuses then separates from plasma membrane with radial arms. This animation was created on the basis of Tominaga, Naitoh and Allen, 1999, J. Cell Sci., 122, 3733- 3745.
The processes of the digestive cycle in P. multimicronucleatum. This animation was created on the basis of Allen and Fok, 2000, Int. Rev. Cytol., 198, 277-318.
Schematic diagram of double filtering method for cell washing created by PowerPoint.
Schematic diagram of tree method for making sterile strain of Paramecium created by PowerPoint. Related paper information: https://doi.org/10.18980/jjprotozool.JJP16-05
Schematic diagram depicting the left side, dorsal side, right side, and ventral side of Paramecium created by PowerPoint.
Schematic diagram depicting the left side, dorsal side, and right side of the oral apparatus in Paramecium created by PowerPoint. Relataed paper information: J. Euk. Microbiol., 48(6): 640-646
Morphology of the micronucleus and macronucleus during cell division in Paramecium caudatum with anti α-tubulin antibody (mAb N356) and DAPI staining.
Schematic diagram of nuclear morphology changes during the conjugation process of Parmecium caudatum.
Morphology of the micronucleus and macronucleus during the conjugation process of Paramecium caudatum with anti α-tubulin antibody (mAb N356) and DAPI staining.
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