Since 2010, an increased occurrence of high atmospheric temperatures (over 25°C) and a gradual decline of daily temperature variations in the winter season was recorded in essential aquaculture areas across the south of Taiwan. However, extreme winter cold fronts (atmospheric temperature < 10°C) in Taiwan always resulted in large-scale disease, death, and economic loss in the aquaculture industry. According to our systematic metabolic profiling method, we found that the transgenerational experience of ambient temperature perturbations can lead to adaptive metabolic allocation features in the liver of tropical tilapia. Because global warming may result in less thermal variation each year, the stabilized ambient temperature may gradually cause tropical tilapia to exhibit lower energy conservation. In addition to those habitants in cold and temperate regions, a lack of cold exposure to multiple generations of fish may decrease the native cold-tolerance traits of subtropical/tropical organisms; this notion has not been previously explored in terms of the biological effects of anthropogenic climate change.