Research   

The primary research interest of the Miskolci lab is to study the metabolic regulation of innate immunity during inflammation and tissue repair in a whole organism, using zebrafish (Danio rerio) as an in vivo model. We use larval zebrafish models of caudal fin injury in combination with various imaging modalities, including standard confocal spinning disk microscopy of fluorescent reporters, second harmonic generation microscopy (SHG), and autofluorescence lifetime imaging microscopy (FLIM). Optical transparency during larval stages makes zebrafish amenable to live imaging by most microscopy applications. We take mainly a quantitative imaging-based approach to preserve cells in their native microenvironment and allow single cell-based analyses. We complement quantitative imaging with traditional biochemical and multi-omics approaches. We are interested in 1) elucidating the pathways that regulate intrinsic intracellular metabolism, 2) understanding how intracellular metabolism regulates effector functions, 3) understanding how cellular cross-talk modulates metabolism in the tissue niche and 4) developing and applying single cell-based approaches to study the intracellular metabolism of immune cells in situ.