Research Tools & Techniques

Cell and Molecular Biology: Gene cloning, Mammalian and bacterial cell culture, Protein expression and purification, Competent cells preparation and transformation, Nucleic acids (DNA & RNA) isolation and purification, transient transfection in mammalian cells, Over expression and purification of the Cytoplasmic, Inclusion bodies and Peripheral and Integral membrane proteins. Antibody purification, Preparation of Fabs.

Preparation and standardization of Lipid Nanodiscs (MSP and peptide based nanodiscs). Reconstitution of Membrane Proteins and Peptides in detergent micelles, bicelles and lipid nanodiscs.

Biophysics: Transmission Electron Microscopy (TEM), Flow cytometry (BD-Fortessa), Analytical ultracentrifugation (AUC), Circular dichroism (CD), FT-IR, Fluorescence spectroscopy, Dynamic light scattering (DLS) and Small Angle X-ray Scattering (SAXS).

Biochemistry: Metal affinity, Size exclusion, Ion exchange and Reverse phase chromatography, Gel electrophoresis (SDS-PAGE, Native PAGE and Agarose) and Western blotting, ELISA-assay.

Peptide/Protein NMR Spectroscopy: Data collection using standard Pulse programs in one and multidimensions (2D and 3D), processing and analysis of multidimensional NMR data.

Computational: Molecular Dynamics Simulations (all-atom/coarse-grained) using NAMD, CHARMM-GUI, ProDy and VMD. Structure calculation, analysis and docking simulations: CYANA, CS-ROSETTA, PyMOL, MolMol, HADDOCK and AutoDock etc.