Publications

Original papers (*Corresponding author)

1. Poonperm R, Ichihara S, Miura H, Tanigawa A, Nagao K, Obuse C, Sado T, *Hiratani I.  Replication dynamics identifies the folding principles of the inactive X chromosome. Nat Struct Mol Biol. 2023 Aug;30(8):1224-1237. doi: 10.1038/s41594-023-01052-1. Epub 2023 Aug 10.

2. Ichihara S, *Nagao K, Sakaguchi T, Obuse C, *Sado T.  SmcHD1 underlies the formation of H3K9me3 blocks on the inactive X chromosome in mice. Development. 2022 Aug 1;149(15):dev200864. doi: 10.1242/dev.200864. Epub 2022 Aug 2.

3. Matsuura R, Nakajima T, Ichihara S, *Sado T. Ectopic splicing disturbs the function of Xist RNA to establish the stable heterochromatin state. Front Cell Dev Biol. 2021 Oct 14; 9: 751154. doi: 10.3389/fcell.2021.751154

4. Ohishi H, Au Yeung WK, Unoki M, Ichiyanagi K, Fukuda K, Maenohara S, Shirane K, Chiba H, Sado T, and *Sasaki H. Characterization of genetic-origin-dependent monoallelic expression in mouse embryonic stem cells. Genes Cells. 2020 Jan;25(1):54-64. doi: 10.1111/gtc.12736. Epub 2019 Dec 11.

5. Hosoi Y, Soma M, Shiura H, Sado T, Hasuwa H, Abe K, Kohda T, Ishino F, and *Kobayashi S. Female mice lacking Ftx lncRNA exhibit impaired X-chromosome inactivation and a microphthalmia-like phenotype. Nat Commun. 2018 Oct 31;9(1):4618. doi: 10.1038/s41467-018-07100-5.

6. Sakakibara Y, *Nagao K, Blewitt M, Sasaki H, Obuse C, and *Sado T. Role of SmcHD1 in establishment of epigenetic states required for the maintenance of the X-inactivated state in mice. Development. 2018 Sep 25;145(18). pii: dev166462. doi: 10.1242/dev.166462

7. Chigi Y, Sasaki H, *Sado T. The 5' region of Xist RNA has the potential to associate with chromatin through the A-repeat. RNA. 2017 Dec;23(12):1894-1901. doi: 10.1261/rna.062158.117. Epub 2017 Sep 22. 

8. Sakata Y, Nagao K, Hoki Y, Sasaki H, Obuse C, *Sado T. Defects in dosage compensation impact global gene regulation in the mouse trophoblast. Development. 2017 Aug 1;144(15):2784-2797. doi: 10.1242/dev.149138. 

9. Fukuda A, Mitani A, Miyashita T, Sado T, Umezawa A, *Akutsu H. Maintenance of Xist Imprinting Depends on Chromatin Condensation State and Rnf12 Dosage in Mice. PLoS Genet. 2016 Oct 27;12(10):e1006375. doi: 10.1371/journal.pgen.1006375. eCollection 2016 Oct. 

10. Sakaguchi T, Hasegawa Y, Brockdorff N, Tsutsui K, Tsutsui KM, *Sado T, *Nakagawa S. Control of Chromosomal Localization of Xist by hnRNP U Family Molecules. Dev Cell. 2016 Oct 10;39(1):11-12. doi: 10.1016/j.devcel.2016.09.022. 

11. Amakawa Y, Sakata Y, Hoki Y, Arata S, Shioda S, Fukagawa T, Sasaki H, *Sado T. A new Xist allele driven by a constitutively active promoter is dominated by Xist locus environment and exhibits the parent-of-origin effects. Development. 2015 Dec 15;142(24):4299-4308. doi: 10.1242/dev.128819. Epub 2015 Oct 28. 

12. Sarkar MK, Gayen S, Kumar S, Maclary E, Buttigieg E, Hinten M, Kumari A, Harris C, Sado T, *Kalantry S. An Xist-activating antisense RNA required for X-chromosome inactivation. Nat Commun. 2015 Oct 19;6:8564. doi: 10.1038/ncomms9564. 

13. Pasque V, Tchieu J, Karnik R, Uyeda M, Sadhu Dimashkie A, Case D, Papp B, Bonora G, Patel S, Ho R, Schmidt R, McKee R, Sado T, Tada T, Meissner A, *Plath K. X chromosome reactivation dynamics reveal stages of reprogramming to pluripotency. Cell. 2014 Dec 18;159(7):1681-1697. doi: 10.1016/j.cell.2014.11.040. 

14. Shinagawa T, Takagi T, Tsukamoto D, Tomaru C, Huynh LM, Sivaraman P, Kumarevel T, Inoue K, Nakato R, Katou Y, Sado T, Takahashi S, Ogura A, Shirahige K, *Ishii S. Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells. Cell Stem Cell. 2014 Feb 6;14(2):217-227. doi:10.1016/j.stem.2013.12.015.

15. Kagawa N, Hori T, Hoki Y, Hosoya O, Tsutsui K, Saga Y, Sado T, *Fukagawa T. The CENP-O complex requirement varies among different cell types. Chromosome Res. 2014 Sep;22(3):293-303. doi: 10.1007/s10577-014-9404-1. Epub 2014 Jan 31. 

16. Nozawa RS, Nagao K, Igami KT, Shibata S, Shirai N, Nozaki N, Sado T, Kimura H, *Obuse C. Human inactive X chromosome is compacted through a PRC2-independent SMCHD1-HBiX1 pathway. Nat Struct Mol Biol. 2013 May;20(5):566-573. doi:10.1038/nsmb.2532. Epub 2013 Mar 31. 

17. Yamamoto Y, Watanabe T, Hoki Y, Shirane K, Li Y, Ichiiyanagi K, Kuramochi-Miyagawa S, Toyoda A, Fujiyama A, Oginuma M, Suzuki H, Sado T, Nakano T, *Sasaki H. Targeted gene silencing in mouse germ cells by insertion of a homologous DNA into a piRNA generating locus. Genome Res. 2013 Feb;23(2):292-299. doi: 10.1101/gr.137224.112. Epub 2012 Nov 6. 

18. Hoki Y, Ikeda R, Mise N, Sakata Y, Ohhata T, Sasaki H, Abe K, *Sado T. Incomplete X-inactivation initiated by a hypomorphic Xist allele in the mouse. Development. 2011 Jul;138(13):2649-2659. doi: 10.1242/dev.061226. Epub 2011 May 25.

19. Watanabe T, Tomizawa S, Mitsuya K, Totoki Y, Yamamoto Y, Kuramochi-Miyagawa S, Iida N, Hoki Y, Murphy PJ, Toyoda A, Gotoh K, Hiura H, Arima T, Fujiyama A, Sado T, Shibata T, Nakano T, Lin H, Ichiyanagi K, Soloway PD, *Sasaki H. Role for piRNAs and noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus. Science. 2011 May 13;332(6031):848-852. doi:10.1126/science.1203919. 

20. Watanabe T, Chuma S, Yamamoto Y, Kuramochi-Miyagawa S, Totoki Y, Toyoda A, Hoki Y, Fujiyama A, Shibata T, Sado T, Noce T, Nakano T, Nakatsuji N, Lin H, *Sasaki H. MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline. Dev Cell. 2011 Mar 15;20(3):364-375. doi:10.1016/j.devcel.2011.01.005. 

21. Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, *Ogura A.  Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer. Science. 2010 Oct 22;330(6003):496-499. doi:10.1126/science.1194174. Epub 2010 Sep 16. 

22. Inoue K, Ogonuki N, Mekada K, Yoshiki A, Sado T, *Ogura A. Sex-reversed somatic cell cloning in the mouse. J Reprod Dev. 2009 Oct;55(5):566-569. Epub 2009 Jul 14. 

23. Hoki Y, Kimura N, Kanbayashi M, Amakawa Y, Ohhata T, Sasaki H, *Sado T. A proximal conserved repeat in the Xist gene is essential as a genomic element for X-inactivation in mouse. Development. 2009 Jan;136(1):139-146. doi: 10.1242/dev.026427. Epub 2008 Nov 26. 

24. Nesterova TB, Popova BC, Cobb BS, Norton S, Senner CE, Tang YA, Spruce T, Rodriguez TA, Sado T, Merkenschlager M, *Brockdorff N. Dicer regulates Xist promoter methylation in ES cells indirectly through transcriptional control of Dnmt3a. Epigenetics Chromatin. 2008 Oct 27;1(1):2. doi: 10.1186/1756-8935-1-2. 

25. Chiba H, Hirasawa R, Kaneda M, Amakawa Y, Li E, Sado T, *Sasaki H. De novo DNA methylation independent establishment of maternal imprint on X chromosome in mouse oocytes. Genesis. 2008 Dec;46(12):768-774. doi: 10.1002/dvg.20438. 

26. Ohhata T, Hoki Y, Sasaki H, *Sado T. Crucial role of antisense transcription across the Xist promoter in Tsix-mediated Xist chromatin modification. Development. 2008 Jan;135(2):227-235. Epub 2007 Dec 5. 

27. *Sado T, Hoki Y, Sasaki H. Tsix defective in splicing is competent to establish Xist silencing. Development. 2006 Dec;133(24):4925-4931. Epub 2006 Nov 15. 

28. Ohhata T, Hoki Y, Sasaki H, *Sado T. Tsix-deficient X chromosome does not undergo inactivation in the embryonic lineage in males: implications for Tsix-independent silencing of Xist. Cytogenet Genome Res. 2006;113(1-4):345-349. 

29. *Sado T, Hoki Y, Sasaki H. Tsix silences Xist through modification of chromatin structure. Dev Cell. 2005 Jul;9(1):159-165. 

30. Purbowasito W, Suda C, Yokomine T, Zubair M, Sado T, Tsutsui K, *Sasaki H. Large-scale identification and mapping of nuclear matrix-attachment regions in the distal imprinted domain of mouse chromosome 7. DNA Res. 2004 Dec 31;11(6):391-407.

31. Ohhata T, Tachibana M, Tada M, Tada T, Sasaki H, Shinkai Y, *Sado T. X-inactivation is stably maintained in mouse embryos deficient for histone methyl transferase G9a. Genesis. 2004 Nov;40(3):151-156.

32. Kaneda M, Okano M, Hata K, Sado T, Tsujimoto N, Li E, *Sasaki H. Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting. Nature. 2004 Jun 24;429(6994):900-903. 

33. *Sado T, Okano M, Li E, Sasaki H. De novo DNA methylation is dispensable for the initiation and propagation of X chromosome inactivation. Development. 2004 Mar;131(5):975-982. 

34. *Sado T, Li E, Sasaki H. Effect of TSIX disruption on XIST expression in male ES cells. Cytogenet Genome Res. 2002;99(1-4):115-118. 

35. Shirohzu H, Kubota T, Kumazawa A, Sado T, Chijiwa T, Inagaki K, Suetake I, Tajima S, Wakui K, Miki Y, Hayashi M, Fukushima Y, *Sasaki H. Three novel DNMT3B mutations in Japanese patients with ICF syndrome. Am J Med Genet. 2002 Sep 15;112(1):31-37. 

36. Takebayashi S, Nakao M, Fujita N, Sado T, Tanaka M, Taguchi H, *Okumura K. 5-Aza-2'-deoxycytidine induces histone hyperacetylation of mouse centromeric heterochromatin by a mechanism independent of DNA demethylation. Biochem Biophys Res Commun. 2001 Nov 9;288(4):921-926.

37. Sado T, Wang Z, Sasaki H, *Li E. Regulation of imprinted X-chromosome inactivation in mice by Tsix. Development. 2001 Apr;128(8):1275-1286. 

38. Grandjean V, O'Neill L, Sado T, Turner B, *Ferguson-Smith A. Relationship between DNA methylation, histone H4 acetylation and gene expression in the mouse imprinted Igf2-H19 domain. FEBS Lett. 2001 Jan 19;488(3):165-169. 

39. Sado T, Fenner MH, Tan SS, Tam P, Shioda T, *Li E. X inactivation in the mouse embryo deficient for Dnmt1: distinct effect of hypomethylation on imprinted and random X inactivation. Dev Biol. 2000 Sep 15;225(2):294-303. 

40. Nishita Y, Sado T, Yoshida I, *Takagi N. Effect of CpG methylation on expression of the mouse imprinted gene Mest. Gene. 1999 Jan 21;226(2):199-209. 

41. Schuster-Gossler K, Bilinski P, Sado T, Ferguson-Smith A, *Gossler A. The mouse Gtl2 gene is differentially expressed during embryonic development, encodes multiple alternatively spliced transcripts, and may act as an RNA. Dev Dyn. 1998 Jun;212(2):214-228.

42. Tada T, Tada M, Hilton K, Barton SC, Sado T, Takagi N, *Surani MA. Epigenotype switching of imprintable loci in embryonic germ cells. Dev Genes Evol. 1998 Feb;207(8):551-561. 

43. Nishita Y, Yoshida I, Sado T, *Takagi N. Genomic imprinting and chromosomal localization of the human MEST gene. Genomics. 1996 Sep 15;36(3):539-542. 

44. Sado T, Tada T, *Takagi N. Mosaic methylation of Xist gene before chromosome inactivation in undifferentiated female mouse embryonic stem and embryonic germ cells. Dev Dyn. 1996 Apr;205(4):421-434. 

45. Mise N, Sado T, Tada M, Takada S, *Takagi N. Activation of the inactive X chromosome induced by cell fusion between a murine EC and female somatic cell accompanies reproducible changes in the methylation pattern of the Xist gene. Exp Cell Res. 1996 Mar 15;223(2):193-202. 

46. *Nakajima N, Sado T. Nucleotide sequence of a mouse lamin A cDNA and its deduced amino acid sequence. Biochim Biophys Acta. 1993 Jan 23;1171(3):311-314. 

47. Sado T, Nakajima N, Tada M, *Takagi N.A novel mesoderm-specific cDNA isolated from a mouse embryonal carcinoma cell line.  Dev Growth Diff. 1993 Oct; 35:550-559. 

Reviews

1. *Sado T. Does XIST safeguard against sex-biased human diseases? Mol Cell. 2021 Apr 15;81(8):1598-1600. doi: 10.1016/j.molcel.2021.03.036.

2. *Sado T. What makes the maternal X chromosome resistant to undergoing imprinted X inactivation? Philos Trans R Soc Lond B Biol Sci. 2017 Nov 5;372(1733). pii: 20160365. doi: 10.1098/rstb.2016.0365. Review. 

3. Nakajima T, *Sado T. Current view of the potential roles of proteins enriched on the inactive X chromosome. Genes Genet Syst. 2014;89(4):151-157. doi:10.1266/ggs.89.151. Review. 

4. *Sado T, Sakaguchi T. Species-specific differences in X chromosome inactivation in mammals. Reproduction. 2013 Aug 12;146(4):R131-139. doi: 10.1530/REP-13-0173. Print 2013 Oct. Review.

5. *Sado T, Brockdorff N. Advances in understanding chromosome silencing by the long non-coding RNA Xist. Philos Trans R Soc Lond B Biol Sci. 2013 Jan 5;368(1609):20110325. doi: 10.1098/rstb.2011.0325. Review. 

6. Kaneda M, Sado T, Hata K, Okano M, Tsujimoto N, Li E, *Sasaki H. Role of de novo DNA methyltransferases in initiation of genomic imprinting and X-chromosome inactivation. Cold Spring Harb Symp Quant Biol. 2004;69:125-129. Review. 

7. Sado T, *Ferguson-Smith AC. Imprinted X inactivation and reprogramming in the preimplantation mouse embryo. Hum Mol Genet. 2005 Apr 15;14 Spec No 1:R59-64. Review.