Volume 1 (2025) 

The effects of different nitrogen application patterns on soil microbial diversity and community structure in the rice-Brassica campestris rotation system

Guangda Shi, Wenxuan Tang, Yuebin Cong

Volume 1 (2025), Article ID: eip1v0117a  

Published: 2025-01-17 (Received: 2024-11-23; Revised: 2025-01-05; Accepted: 2025-01-15)

DOI:  https://doi.org/10.5281/zenodo.15543238 

Citation

Shi G, Tang W, Cong Y. The effects of different nitrogen application patterns on soil microbial diversity and community structure in the rice-Brassica campestris rotation system. Engineering Innovation and Practice, 2025, 1, eip1v0117a.

Abstract

To investigate the effects of different nitrogen application patterns on soil microbial community diversity and structure in the rice-Brassica campestris rotation system, this study established four treatments: full nitrogen application, reduced nitrogen application, combined nitrogen and organic fertilizer application, and no nitrogen application. High-throughput sequencing technology was used to systematically analyze bacterial and fungal communities’ Alpha diversity, Beta diversity, and community composition. The results showed that reduced nitrogen application and combined nitrogen-organic fertilizer application significantly increased the species richness and evenness of bacteria and fungi. Among the treatments, organic fertilizer application had the most pronounced effect on optimizing microbial community structure, significantly enhancing the abundance of functional bacterial groups such as Actinobacteria and Firmicutes. It also positively regulated fungal groups such as Ascomycota and Zygomycota. Beta diversity analysis revealed that different nitrogen application treatments significantly altered soil microbial community composition, with these changes closely associated with environmental factors such as soil C: N ratio and pH. The study demonstrated that reduced nitrogen application combined with organic fertilizer has a synergistic effect on optimizing soil microbial community structure and improving soil ecological functions. These findings provide a theoretical basis for the scientific fertilization management of the rice-Brassica campestris rotation system and offer scientific references for promoting green agricultural practices that harmonize nitrogen reduction with soil health.

Keywords

soil microbial diversity, nitrogen application, rice rotation system, organic fertilizer, microbial community structure

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