Biomedical Research

Assisting confocal microscope Imaging of Breast cancer cells; Microfluidic chip; Fibroblast Cell experimental design; Fluorescence Imaging; Experimental Design, Microscope Imaging, Scanning electron microscope imaging.

Paper Editing & writing

Editing Scientific Journal Papers before submission, working with and assisting labmates with micro-device fabrication; and microscope Plasmonic chip design and metallization (physical vapor deposition, sputter, e-beam vapor deposition).

1. Volunteer-USF-Innovative Biomedical Instruments & Systems (IBIS)

Microscope Imaging & Videos

• Red Blood Cells

• White blood Cells

• Platelets

• Bacteria

2. Optical Fiber face Photo Resist spinning device prototype

3. Optical Fiber face Photo Resist Spinning Project.

4. Optical Fiber face photo-resist spinning process design.

5. Optical Fiber face photoresist spinning experimental design.

6. Optical Fiber face photoresist spinning Microscope imaging.

7. Optical Fiber face photoresist spinning SEM imaging.

8. Optical Fiber Face Photoresist spinning Optical profilometry.

9. Optical Fiber Face photoresist Spinning surface roughness analysis.

10. Optical Fiber Face photoresist spinning surface angle and cleave angle.

11. USF-Undergraduate Research Assistant-IBIS

12. Metrology

13. SEM Imaging

14. Experiment Documentation

15. Experimental Design

16. Data Organization

17. Data Labeling hierarchies

18. Nano-scale Endoscope

19. Prototype Research Prototype Design FIB Device design SiO2 nano wire experiments

20. Prototype Fabrication

21. Physical vapor deposition

22. FIB milling

23. HF Etch

24. Experimental Design

25. Optical fiber coupling device

26. Optical fiber coupling device prototype 1 design 1.

27. Prototype design-prefabrication Microscope Slide measurements Dicing saw width Optical Fiber width Coupler Trench Spacing Dicing saw height

28. Optical fiber coupling device prototype 1 fabrication.

29. Dicing saw leveling techniques. Dicing saw depth calculations. Dicing saw depth to trench width calculations

30. Optical fiber coupling device prototype 1 metrology.

31. Optical microscope imaging Optical microscope measurements Dicing saw trench measurements. Optical fiber measurements. Optical fiber inside trench imaging Optical microscope fiber face imaging before placing in coupling device. Optical microscope fiber face imaging after placing in coupling device.

32. Optical Fiber coupling Device prototype 1 Experimentation.

33. Coupled light into two cleaved optical fiber cores using microscope slide coupling device prototype positioned horizontally. Coupled light into two cleaved optical fiber cores using microscope slide coupling device prototype positioned vertically. One optical fiber coupled to laser 2nd optical fiber placed inside coupling device. Placed 4 fibers in the optical fiber coupling device prototype for coupling.

34. Optical fiber coupling device design

35. Optical Fiber Face sputtering experiments.

36. Optical Fiber Face E-beam Vaporization.

37. Optical Fiber face FIB milling.

38. Faces 1-4 

39. Placed in optical fiber coupling device with sputtered faces.

40. ***Need to confirm sputter or e-beam vaporization for optical fiber film coating***.

41. Milled nanoscale holes in sputter optical fiber faces.

42. SEM Imaging of optical fiber faces.

43. Placed milled optical fibers in the optical fiber coupling device.

44. Checked milled/sputtered optical fiber surfaces for damage after placing in optical fiber coupling device trenches 1-4.

45. Optical Fiber cleaving Experimental design.

46. Optical Fiber face cleaving angle experiments. Optical Fiber Face Cleaving microscope imaging.

47. Optical Fiber Face cleaving Optical Profilometry.

48. Optical Fiber Face Cleaving SEM Imaging.

49. Optical Fiber Face Cleaving Experiments documentation.

50. USF-IBIS Research Lab Equipment Purchasing Research.

51. Optical Fiber Cleaver-Research & Pricing.

52. Optical Fiber cleaver research & pricing documentation.

53. Optical Fiber Fusion Splicer Research & Pricing.

54. Optical Fiber Cleaver & Fusion splicer manufacturer & retailer emailing.

55. Initial contact

56. Initial quote 

57. Optical Fiber Cleaver & Fusion splicer purchase contract negotiation.

58.  Cleared Optical Fiber Cleaver & Fusion splicer Purchase contract with Principle Investigator.

59. Fusion splicer & cleaver unpacking

60. Read cleaving & splicing directions

61. Fiber to fiber splicing

62. Created custom process for fiber splicing to a 4 cm optical fiber.

63. Nanoscale Endoscope prototype 2 design- etching

64. HF Etch Experiments

65. Optical Fiber materials publication Research.

66. Optical Fiber etching research.

67. Semiconductor Grade HF handling Procedures.

68. Semiconductor Grade HF waste Procedure.

69. Si-02 experimental design & documentation

70. Si-02 etch rate calculations

71. Initial experiment parameters

72. Publications research

73. Si-o2 fabrication experiments

74. Hf etch Experiment set up design

75. HF si-o2 etching experiment

76. Etch time

77. SEM image thickness measurements 

78. Taper angle  measurements

79. Taper length measurements

80. Etch rate calculations

81. Experimental iterations 

82. Nanoscale device confirmation

83. Breast Cancer cell confocal microscopy experiments.

84. Worked with Moffit cancer center

85. Fluorescence imaging

86. Broken fiber

87. Optical trapping effects?

88. Thermo-plasmonic device prototype

89. Initial Plasmonics research

90. Sputtered film absorption research

91. Au film adherence research.

92. Au Sputtered film absorption spectrum experimentation.

93. Au cr sputtered film absorption spectrum research.

94. Au cr sputtered film absorption spectrum experiments.

95. Au sputtered film absorption spectrum research.

96. Au sputtered film absorption spectrum experiments.

97. Experimental sputtered film spectrum absorption experimental results vs. simulation.

98. Physical vapor deposition film initial experimentation and design

99. E-beam vapor film deposition

100. Sputter film deposition

101. Horizontal sputter film

102. Optical profilometry?

103. SEM?

104. Vertical sputter film

105. Optical profilometry?

106. SEM?

107. Super Resolution Microscopy

108. Super Resolution Microscopy Research.

109. CCD Camera sensor Technology Research.

110. Super Resolution microscopy Vendor Demonstration.

111. Super Resolution microscopy in-lab vendor demonstration.

112. Compare & contrast vendor technology w/ CCD sensor microscope add-on technology.

113. Infrared Camera

114. infrared Camera technology research.

115. infrared Camera sensor sensitivity testing.

116. Spatial resolution sensitivity experiments.

117. Thermal resolution sensitivity experiments.

Bio-Medical Engineering Research

Optical Fibers

• Cleaving

  • Fiber Cleave Face Analysis

    • Optical Profilometry

  • Fusion Splicing

  • Resist Spinning

    • Optical Profilometry

Nanoscale endoscope

• Experimental Design

  • HF Etching

  • SEM Imaging

  • Optical Characterization

  • Prototyping

    • FIB Prototyping

      • Milling

    • Au Sputting

      • Sputtering holder design

    • Dicing Saw

      • Optical fiber holders

      • Optical Fiber Couplers

    • Sputter Film characterization

• Confocal microscopy Experiments

  • Fiber & cell Imaging

  • Optical trapping effects/ thermofluidic trapping effects

• Thermofluidic device design &experiments

• Paper writing