Publications
2022
Azinheiro, S., Roumani, F., Prado, M., & Garrido-Maestu, A. (2022). Rapid Same-Day Detection of Listeria monocytogenes, Salmonella spp., and Escherichia coli O157 by Colorimetric LAMP in Dairy Products. Food Analytical Methods, 1-13.
https://doi.org/10.1007/s12161-022-02345-9
Purwidyantri, A., Ipatov, A., Domingues, T., Borme, J., Martins, M., Alpuim, P., Prado, M. (2022). Programmable graphene-based microfluidic sensor for DNA detection.
Sensors and Actuators B: Chemical, 132044
https://doi.org/10.1016/j.snb.2022.132044
Garrido-Maestu, A., Prado, M. (2022). Naked‐eye detection strategies coupled with isothermal nucleic acid amplification techniques for the detection of human pathogens.
Comprehensive Reviews in Food Science and Food Safety 21 (2), 1913-1939
https://doi.org/10.1111/1541-4337.12902
Hsu, C.H., Gupta, A.K., Purwidyantri, A., Prabowo, B.A., Chen, C.H., Chuang, C.C., Tian, Y.C., and Lai, C.S. Alzheimer’s disease Sensor utilizing Simple Electrochemical Oxidation-Reduction Cycle for Au Electrode.
Chemosensors 2022, 10(3), 94
https://doi.org/10.3390/chemosensors10030094
Roumani, F., Azinheiro, S., Rodrigues, C., Barros-Velázquez, J., Garrido-Maestu, A., Prado, M. (2022).
Development of a Panfungal Recombinase Polymerase Amplification (RPA) Method Coupled with Lateral Flow Strips for the Detection of Spoilage Fungi.
Food Analytical Methods, 2022
https://doi.org/10.1007/s12161-022-02242-1
Lozano-León, A., García-Omil, C., Rodríguez-Souto, R.R., Lamas, A., Garrido-Maestu, A. (2022).
An Evaluation of the Pathogenic Potential, and the Antimicrobial Resistance, of Salmonella strains Isolated from Mussels.
Microorganisms, 2022
https://doi.org/10.3390/microorganisms10010126
Roumani, F., Gómez, S., Rodrigues, C., Barros-Velázquez, J., Garrido-Maestu, A., Prado, M. (2022).
Development and evaluation of a real-time fluorescence, and naked-eye colorimetric, loop-mediated isothermal amplification-based method for the rapid detection of spoilage fungi in fruit preparations.
Food Control, 2022
https://doi.org/10.1016/j.foodcont.2021.108784
Roumani, F., Azinheiro, S., Rodrigues, C., Barros-Velázquez, J., Garrido-Maestu, A., Prado, M. (2022).
Development of a real-time PCR assay with an internal amplification control for the detection of spoilage fungi in fruit preparations.
Food Control, 2022
https://doi.org/10.1016/j.foodcont.2021.108783
Azinheiro, Carvalho, J., Fuciños, P., Pastrana, L., Prado, M., Garrido-Maestu, A. (2022).
Short pre-enrichment and modified matrix lysis. A comparative study towards same-day detection of Listeria monocytogenes.
LWT, 2022
https://doi.org/10.1016/j.lwt.2021.112900
Azinheiro, S., Roumani, F., Rodríguez-Lorenzo, L., Carvalho, J., Prado, M., Garrido-Maestu, A. (2022).
Combination of Recombinase Polymerase Amplification with SYBR Green I for naked-eye same-day detection of Escherichia coli O157:H7 in ground meat.
Food Control, 2022
https://doi.org/10.1016/j.foodcont.2021.108494
Azinheiro, S., Ghimire, D., Carvalho, J., Prado, M., Garrido-Maestu, A. (2022).
Next-day detection of viable Listeria monocytogenes by multiplex reverse transcriptase real-time PCR.
Food Control, 2022
https://doi.org/10.1016/j.foodcont.2021.108593
2021
Yadav, S., Carvalho, J., Trujillo, I., Prado, M. (2021).
Microsatellite Markers in Olives (Olea europea L.): Utility in the Cataloging of Germplasm, Food Authenticity and Traceability Studies.
Foods, 2021
https://doi.org/10.3390/foods10081907
Elumalai, M., Ipatov, A., Carvalho, Guerreiro, J.R.L., Prado, M. (2021).
Dual colorimetric strategy for specific DNA detection by nicking endonuclease-assisted gold nanoparticle signal amplification.
Analytical and Bioanalytical Chemistry, 2021
https://doi.org/10.1007/s00216-021-03564-5
Naseri, M., Halder, A., Mohammadniaei, M., Prado, M., Ashley, J., Sun, Y. (2021).
A multivalent aptamer-based electrochemical biosensor for biomarker detection in urinary tract infection.
Electrochimica Acta, 2021
https://doi.org/10.1016/j.electacta.2021.138644
Guerreiro, J.R.L., Ipatov, A., Carvalho, J., Toldrá, A., Prado, M. (2021).
Amplified plasmonic and microfluidic setup for DNA monitoring.
Microchimica Acta, 2021
https://doi.org/10.1007/s00604-021-04983-y
Carvalho, J., Yadav, S., Garrido-Maestu, A., Azinheiro, Trujillo, I., Barros-Velázquez, J., Prado, M. (2021).
Evaluation of simple sequence repeats (SSR) and single nucleotide polymorphism (SNP)-based methods in olive varieaties from Northwest of Spain and potential for miniaturization.
Food Chemistry: Molecular Sciences, 2021
https://doi.org/10.1016/j.fochms.2021.100038
Azinheiro, S., Roumani, F., Carvalho, J., Prado, M., Garrido-Maestu, A. (2021).
Suitability of the MinION long read sequencer for semi-targeted detection of foodborne pathogens.
Analytica Chimica Acta, 2021
https://doi.org/10.1016/j.aca.2021.339051
Roumani, F., Azinheiro, S., Sousa, H., Sousa, A., Timéteo, M., Varandas, T., Fonseca-Silva, D., Baldaque, I., Carvalho, J., Prado, M., Garrido-Maestu, A. (2021).
Optimization and Clinical Evaluation of a Multi-Target Loop-Mediated Isothermal Amplification Assay for the Detection of SARS-CoV-2 in Nasopharyngeal Samples.
Viruses, 2021, 13(5), 940
https://doi.org/10.3390/v13050940
Lozano-León, A., Rodríguez-Souto, R. R., González-Escalona, N., Llovo-Taboada, J., Iglesias-Canle, J., Álvarez-Castro, A., & Garrido-Maestu, A. (2021).
Detection, molecular characterization, and antimicrobial susceptibility, of Campylobacter spp. isolated from shellfish.
Microbial Risk Analysis, 100176.
https://doi.org/10.1016/j.mran.2021.100176
Carvalho, J.; Garrido-Maestu, A.; Azinheiro, S.; Fuciños, P.; Barros-Velázquez, J.; De Miguel, R. J.; Gros, V.; Prado, M. (2021)
Faster monitoring of the invasive alien species (IAS) Dreissena polymorpha in river basins through isothermal amplification
Scientifid Reports, 11, 10175 (2021)
https://doi.org/10.1038/s41598-021-89574-w
Roumani, F.; Azinheiro, S.; Carvalho, J.; Prado, M. Garrido-Maestu, A. (2021)
Loop-mediated isothermal amplification combined with immunomagnetic separation and propidium monoazide for the specific detection of viable Listeria monocytogenes in milk products, with an internal amplification control
Food Control, 125, July 2021, 107975
https://doi.org/10.1016/j.foodcont.2021.107975
Carvalho, J.; Diéguez, L.; Ipatov, A.; Guerreiro, J.R.; Garrido-Maestu, A.; Azinheiro, S.; Prado, M. (2021)
Single-use microfluidic device for purification and concentration of environmental DNA from river water.
Talanta 2021, 226, 122109
https://doi.org/10.1016/j.talanta.2021.122109
2020
Garrido-Maestu A., Azinheiro A., Roumani F., Carvalho J., Prado M., (2020)
Application of Short Pre-enrichment, and Double Chemistry Real-Time PCR, Combining Fluorescent Probes and an Intercalating Dye, for Same-Day Detection and Confirmation of Salmonella spp. and Escherichia coli O157 in Ground Beef and Chicken Samples. Frontiers in Microbiology
https://doi.org/10.3389/fmicb.2020.591041
https://www.frontiersin.org/articles/10.3389/fmicb.2020.591041/full?&utm_source=Email_to_authors_&utm_medium=Email&utm_content=T1_11.5e1_author&utm_campaign=Email_publication&field=&journalName=Frontiers_in_Microbiology&id=591041
Azinheiro A., Carvalho J., Prado M., Garrido-Maestu A. (2020)
Multiplex Detection of Salmonella spp., E. coli O157 and L. monocytogenes by qPCR Melt Curve Analysis in Spiked Infant Formula. Microorganisms
https://doi.org/10.3390/microorganisms8091359
https://www.mdpi.com/2076-2607/8/9/1359
Azinheiro A., Carvalho J., Prado M., Garrido-Maestu A. (2020)
Application of Recombinase Polymerase Amplification with Lateral Flow for a Naked-Eye Detection of Listeria monocytogenes on Food Processing Surfaces. Foods
https://doi.org/10.3390/foods9091249
https://www.mdpi.com/2304-8158/9/9/1249
Garrido-Maestu A., Azinheiro A., Fuciños P., Carvalho J., Prado M. (2020)
Comparative study of multiplex real-time recombinase polymerase amplification and ISO 11290-1 methods for the detection of Listeria monocytogenes in dairy products. (Journal article). Food Microbiology
https://doi.org/10.1016/j.fm.2020.103570
https://www.sciencedirect.com/science/article/pii/S0740002020301593?dgcid=rss_sd_all
Garrido-Maestu A., Azinheiro A., Carvalho J., Espiña B., Prado M. (2020).
Evaluation and implementation of commercial antibodies for improved nanoparticle-based immunomagnetic separation and real-time PCR for faster detection of Listeria monocytogenes. (Journal Article). Journal of Food Science and Technology-Mysore
https://doi.org/10.1007/s13197-020-04450-1
https://link.springer.com/article/10.1007/s13197-020-04450-1
Teixeira A., Paris J.L., Roumani F., Diéguez L., Prado M., Espiña B., Abalde-Cela S., Garrido-Maestu A., Rodríguez-Lorenzo L. (2020).
Multifuntional Gold Nanoparticles for the SERS Detection of Pathogens Combined with a LAMP–in–Microdroplets Approach (Journal Article). Materials. https://doi.org/10.3390/ma13081934
https://www.mdpi.com/1996-1944/13/8/1934/htm
Azinheiro, S., Kant, K., Shahbazi, M. A., Garrido-Maestu, A., Prado, M., & Dieguez, L. (2020).
A smart microfluidic platform for rapid multiplexed detection of foodborne pathogens (Journal Article). Food Control, 107242. https://doi.org/10.1016/j.foodcont.2020.107242.
https://www.sciencedirect.com/science/article/pii/S0956713520301584
Alejandro Garrido-Maestu, Sarah Azinheiro, Joana Carvalho, Pablo Fuciños, Marta Prado
Optimized sample treatment, combined with Real-Time PCR, for Same-day detection of E. coli O157 in ground beef and leafy greens (Journal Article). Food Control. DOI: 10.1016/j.foodcont.2019.106790 https://www.sciencedirect.com/science/article/pii/S0956713519303792?via%3Dihub
Gupta, A. K., Hsu, C. H., Purwidyantri, A., Prabowo, B. A., Chiu, K. P., Chen, C. H., ... & Lai, C. S.
ZnO-Nanorod processed PC-SET as the light-harvesting model for plasmontronic fluorescence Sensor (Journal Article). Sensors and Actuators B: Chemical, 307, 127597.
https://doi.org/10.1016/j.snb.2019.127597
https://www.sciencedirect.com/science/article/pii/S0925400519317964
2019
Laura Rodríguez-Lorenzo, Alejandro Garrido-Maestu, Arun K. Bhunia, Begoña Espiña, Marta Prado, Lorena Diéguez Sara Abalde-Cela
Gold Nanostars for the Detection of Foodborne Pathogens via Surface-Enhanced Raman Scattering Combined with Microfluidics (Journal Article). ACS Applied Nano Materials. DOI: 10.1021/acsanm.9b01223. https://pubs.acs.org/doi/abs/10.1021/acsanm.9b01223
Rui Campos, Jérôme Borme, Joana Rafaela Guerreiro, George Machado Jr., Maria Fátima Cerqueira, Dmitri Y. Petrovykh, Pedro Alpuim
Attomolar Label-Free Detection of DNA Hybridization with Electrolyte-Gated Graphene Field-Effect Transistors (Journal Article). ACS Sensors. DOI:0.1021/acssensors.8b00344.
https://pubs.acs.org/doi/abs/10.1021/acssensors.8b00344.
Alejandro Garrido-Maestu, Sarah Azinheiro, Joana Carvalho, Marta Prado
Combination of Immunomagnetic separation and real-time Recombinase Polymerase Amplification (IMS-qRPA) for specific detection of Listeria monocytogenes in smoked salmon samples (Journal Article). Journal of Food Science, DOI: 10.1111/1750-3841.14662. https://onlinelibrary.wiley.com/doi/abs/10.1111/1750-3841.14662
Lei Wu , Alejandro Garrido-Maestu , Joana R. L. Guerreiro , Sandra Carvalho , Sara Abalde-Cela , Marta Prado, Lorena Diéguez
Amplification-free SERS analysis of DNA mutation in cancer cells with single-base sensitivity (Journal Article). Nanoscale, vol. 11, no 16, p. 7781-7789, 2019. https://pubs.rsc.org/en/content/articlehtml/2019/nr/c9nr00501c
Alejandro Garrido-Maestu, Pablo Fuciños, Sarah Azinheiro, Carla Carvalho, Joana Carvalho, Marta Prado
Specific detection of viable Salmonella Enteritidis by phage amplification combined with qPCR (PAA-qPCR) in spiked chicken meat samples. (Journal Article). Food Control, 99, 79-83, 2019. https://www.sciencedirect.com/science/article/pii/S0956713518306388
Alejandro Garrido-Maestu, David Tomás Fornés, Marta Prado Rodríguez
The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens. (Book Chapter). Foodborne Bacterial Pathogens. Methods in Molecular Biology. https://link.springer.com/protocol/10.1007/978-1-4939-9000-9_3
2018
Joana Carvalho, Gema Puertas, João Gaspar, Sarah Azinheiro, Lorena Diéguez, Alejandro Garrido-Maestu, Manuel Vázquez, Jorge Barros-Velázquez, Susana Cardoso, Marta Prado
Highly efficient DNA extraction and purification from olive oil on a washable and reusable miniaturized device (Journal Article)
Analytica Chimica Acta, 1020 , pp. 30-40, 2018. https://www.sciencedirect.com/science/article/pii/S0003267018303635.
Alejandro Garrido-Maestu, Sarah Azinheiro Pablo Fuciños, Joana Carvalho, Marta Prado
Highly sensitive detection of gluten-containing cereals in food samples by real-time Loop-mediated isothermal AMPlification (qLAMP) and real-time polymerase chain reaction (qPCR) (Journal Article)
Food Chemistry, Volume 246 , pp. Pages 156–163, 2018. https://www.sciencedirect.com/science/article/pii/S0308814617318137?via%3Dihub.
Alejandro Garrido-Maestu, Sarah Azinheiro, Joana Carvalho, Pablo Fuciños, Marta Prado
Development and evaluation of loop-mediated isothermal amplification, and Recombinase Polymerase Amplification methodologies, for the detection of Listeria monocytogenes in ready-to-eat food samples (Journal Article)
Food Control, Volume 86 , pp. Pages 27–34, 2018. https://www.sciencedirect.com/science/article/pii/S0956713517305285.
Alejandro Garrido-Maestu, Sarah Azinheiro, Joana Carvalho, Marta Prado
Rapid and sensitive detection of viable Listeria monocytogenes in food products by a filtration-based protocol and qPCR (Journal Article)
Food Microbiology, 73 , pp. 254-263, 2018. https://www.sciencedirect.com/science/article/pii/S0740002017304112?via%3Dihub.
Sarah Azinheiro, Joana Carvalho, Marta Prado, Alejandro Garrido-Maestu
Evaluation of different genetic targets for Salmonella enterica serovar Enteriditis and Typhimurium, using Loop-mediated isothermal AMPlification for detection in food samples (Journal Article)
Frontiers in Sustainable Food Systems, 2018. https://www.frontiersin.org/articles/10.3389/fsufs.2018.00005/full.
Joana Carvalho, Renato Negrinho, Sarah Azinheiro, Alejandro Garrido-Maestu, Jorge Barros-Velázquez, Marta Prado
Novel approach for accurate minute DNA quantification on microvolumetric solutions (Journal Article)
Microchemical Journal, 138, pp. 540-549, 2018. https://www.sciencedirect.com/science/article/pii/S0026265X17312559.
2017
Alejandro Garrido-Maestu, Sarah Azinheiro, Joana Carvalho, Sara Abalde-Cela, Enrique Carbó-Argibay, Lorena Diéguez, Marek Piotrowski, Yury Kolen'ko, Marta Prado
Combination of microfluidic loop-mediated isothermal amplification with gold nanoparticles for rapid detection of Salmonella spp. in food samples (Journal Article)
Frontiers in Microbiology, 2017. https://www.frontiersin.org/articles/10.3389/fmicb.2017.02159/full.
Alejandro Garrido-Maestu, Pablo Fuciños, Sarah Azinheiro, Joana Carvalho, Marta Prado
Systematic loop-mediated isothermal amplification assays for rapid detection and characterization of Salmonella spp., Enteritidis and Typhimurium in food samples (Journal Article)
Food Control, 80 , pp. Pages 297-306, 2017. https://www.sciencedirect.com/science/article/pii/S0956713517302542.
Stephanie Vial, Youri Berrahal, Marta Prado, Jérôme Wenger
Single-Step DNA Detection Assay Monitoring Dual-Color Light Scattering from Individual Metal Nanoparticle Aggregates (Journal Article)
ACS Sensors , 2 (2) , pp. pp 251–256, 2017. https://pubs.acs.org/doi/abs/10.1021/acssensors.6b00737.
2016
Marta Prado, Begoña Espiña, María Teresa Fernandez-Arguelles, Lorena Diéguez, Pablo Fuciños , Stephanie Vial, Oliveira, Rui Reis, Karola Boehme
Detection of Foodborne Pathogens Using Nanoparticles. Advantages and Trends (Book Chapter)
Antimicrobial Food Packaging, pp. pp.183-202, Jorge Barros-Velazquez, 1st Edition, 2016, ISBN: 978-0-12-800723-5. https://www.sciencedirect.com/science/article/pii/B9780128007235000140.
Marta Prado, Ortea, Stephanie Vial, José Rivas, Pilar Calo-Mata, Jorge Barros-Velázquez
Advanced DNA- and Protein-based Methods for the Detection and Investigation of Food Allergens (Journal Article)
Critical Reviews in Food Science and Nutrition , 56 (Issue 15), pp. 2511-2542, 2016. https://www.tandfonline.com/doi/abs/10.1080/10408398.2013.873767.