IISER Pune Microscopy Facility

Facility in brief

IISER Pune microscopy facility is one of kind in India providing the centralized microscopy facility to different disciplines with in the IISER and out sourcing where different aspirants who are willing to utilize the facility from other institutes and companies in and around Pune.

Facility is also equipped with very high end software analysis like Imaris bitplane, Autoquant, Huygen from SVI etc., allowing users to process and analyze the images in various dimensions. Other than microscopes.

Installation history of different microscopes

IISER-Leica Microimaging centre*:- STED 3X Nanoscope, Upright Multiphoton, SP8 Confocal, DM6 Epifluorescence. Visit the imaging centre link to see more.

Currently IISER Pune microscopy facility have following microscopes

Olympus Live Cell imaging TIRF Microscope:

Olympus CellTIRF microscope has IX81 frame with 10x, 20X, 100X SApo Objectives and 60X, 100X PlanApo TIRF objectives. Lamp source used is MT20 which has Mercury-Xenon lamp with the shutter speed of 1ms. Eight position high speed excitation filter wheel is fitted in the MT20 for fast switching between the excitation wavelengths. There is another eight position high speed filter wheel to collect the emitted light. System is attached with nose piece Piezo for fast and accurate Z-sectioning.

Two cameras are attached to this system one is Hamamatsu OrcaR2 CCD camera and the other is Andor EMCCD camera.

Cell TIRF is attached at the back port of the microscope frame. Cell TIRF can accommodate four laser lines (405nm, 499nm, 561nm) and are completely motorized (controlled by software) to achieve the critical angle.

Stage is covered by Oko lab incubator for providing the environmental conditions for live cell imaging.

Interface software is Xcelence RT

Zeiss LSM 710 Confocal microscope:

LSM 710 confocal microscope has Axio Observer Z1 with 10X, 25X, 40X, 63X, 100X Plan-Apochromat Objectives. X-Cite Metal halide lamp is used for conventional fluorescence microscopy and observation. There are four lasers in this system

(1) Argon(458nm, 488nm, 514nm)

(2) Diode laser(405nm)

(3) DPSS laser(561nm)

(4) HeNe laser(633nm)

There are 3 channels(PMT) for detection of emissions and one of them is 32 spectral detector(QUASAR detector).

Zeiss 710 LSM Confocal/Anisotropy microscope

This system specifications are very much same as above mentioned system. The major difference in this microscope is of Anisotropy module with the polarisation beam splitters in the scanner and filter hood.

10X, 20X, 40X and 63X Plan-Apochromat Objectives are attached to this frame.

There are 2 channels(PMT) for detection of emissions.

Zeiss 780 LSM Confocal/Multiphoton microscope

LSM 780 confocal microscope has Axio Observer Z1 with 10X, 20x, 25X, 40X and 63X Plan-Apochromat Objectives. X-Cite Metal halide lamp is used for conventional fluorescence microscopy and observation. There are five one photon lasers in this system

(1) Argon(458nm, 488nm, 514nm)

(2) Diode laser(405nm)

(3) DPSS laser(561nm)

(4) HeNe laser(594nm)

(5) HeNe laser(633nm)

And for multiphoton, system is attached with Mai-Tai DeepSee femto-second pulsed laser, wavelength ranging from 690nm to 1040nm. LSM 780 scanner is equipped with one GaAsP detector and two PMT. One NDD (PMT) scanner is attached on the right side port of the microscope.

Carl zeiss EVO LS10 Scanning Electron Microscope

Evo LS 10 is a Tungsten emission system with water vapor admittance in the sample chamber, has a extended pressure operation up to 3000Pa and provided with 5 axis motorized stage with software joystick.

Detectors in this system are Secondary Electron (SE) Detector, Variable pressure Secondary Electron (VPSE) 3G detector, LM 4Q Backscattered Detector (BSD).

Additional feature is STEM (Scanning Transmission Electron Microscope) with the separate STEM detector (Single grid solid state STEM detector) and sample holder.

SMART SEM software is used to operate the microscope.