The bacterial RecA protein has known to play an important role in recombination and repair pathways. In the RecA-mediated homologous recombination process, there are two prerequisite steps: nucleation, in which a RecA oligomer consisting of about 6 RecA subunits binds to DNA, and a unidirectional filament extension that proceeds from 5′ to 3′ on single stranded DNA (ssDNA). RecA promotes recombination in a wide range of physiological contexts, depending on the lifestyle of a given bacterial species. Estimates of fork repair frequency vary, but the highest reported rates in a laboratory environment are no more than once per cell per generation. On the contrary, D. radiodurans has been known to survive severe desiccation, an adaptation that also confers resistance to extraordinary levels of ionizing radiation. Between Escherichia coli and Deinococcus radiodurans, DNA repair systems are dramatically different among these two strains known to possess widely divergent lifestyles. A new strain (CC-FR2-10T), Deincoccus ficus (D.ficus), from the rhizosphere of the sacred tree Ficus religiosa L. in Taiwan was found to be Gram-positive, rod-shaped and non-spore-forming. By using phylogenetic analyses utilizing the 16S rRNA gene sequence of the isolate, it indicated that the organism belongs to the genus Deinococcus. D. ficus has been found to survive under basic conditions, and mutation was found upon UV irradiation. Based on these findings, the hypothesis that DNA-damage repair system in D.ficus is different from that in Deinococcus radiodurain has been proposed. Therefore, we would like to combine single molecule approach, spectroscopy assay and biochemistry assay along with sequence analysis to investigate the D. ficus RecA-mediated homologous recombination in vitro in order to obtain the detailed molecular mechanism.