Ann-Shyn Chiang Lab
重要訊息 : 國立清華大學腦科學研究中心江安世老師實驗室歡迎大學以下學生加入進行專題研究，為配合學校行事曆，請於每年 6月前來信申請，並提供一頁 study plan。實驗室會再統一公告相關研究主題，並安排面談和後續申請事宜。
For the summer internships in Prof. Ann-Shyn Chiang's Lab, the ongoing project is Optogenetic valence. The application is open until June each year and all applicants will need to submit a study plan (one page A4).
如何增強長期記憶？清大腦科團隊找到答案
學習一般需要重複多次，才能由暫時的短期記憶固化為永久的長期記憶。神經科學家約30年前就知道，CREB基因轉錄因子在多次間斷式的學習後會活化，進而觸發一系列新蛋白質的合成而形成長期記憶。但為何有些事件我們經歷一次就永久記得? 是不一樣的分子機制嗎? 清華大學腦科學研究中心團隊以果蠅的嗅覺與電擊關聯性學習測試，發現腦神經網路是以一種「雙開關機制」來調控長期記憶的形成。
果蠅有兩個CREB轉錄因子基因，CREBA與CREBB。團隊發現將CREBA轉錄因子表現於左右腦各一顆的DAL神經元，果蠅僅需一次「弱」學習，就可以形成長期記憶。在正常果蠅，長期記憶無法在弱學習後形成是因為DAL神經元內的CREBB蛋白質抑制了CREBA蛋白質活性；當給予十次的「強」訓練後、CREBA基因被活化，並產生新蛋白質解除CREBB蛋白質的抑制作用，且促進下游記憶蛋白的表現，進而形成長期記憶。CREBA/CREBB相互抑制，依學習的強度調控兩者之間的平衡，進而促進或抑制成長期記憶的形成，此發現2021年發表於〈美國國家科學院院刊〉。(more)
論文全文請參閱期刊網站：http://www.pnas.org/。
Drosophila Memory Connectome
To understand how the brain controls behavior and cognition, our interdisciplinary team is the first among the world to construct the 3D fly brain database at single neuron resolution. This makes the team featured in The New York Times article, titled "Decoding the Human Brain, With Help From a Fly". Base on this map, we also found that learning-induced new protein synthesis occur in only few brain neurons for the formation of long-term memory and pulished in Science magzine. Our team will continously focus on understanding how pairing external odor information with punishment or reward forms long-term memory in fruit flies.
本研究團隊於2010年12月發表了解析度到達單細胞層次之果蠅全腦網絡立體圖譜，獲刊於《現代生物學》雜誌，廣受國內外媒體報導，紐約時報更盛讚此研究為人類全腦圖譜解碼之第一步。藉由此圖譜，本團隊更發現為了現儲存長期記憶所引發的蛋白質新生，僅發生在果蠅腦中少數幾顆特定的神經細胞內，並成為國內首位以長篇論文形式發表在《科學》雜誌之研究。
我們會持續針對果蠅長期記憶形成的分子機制以及嗅覺神經網路做更深入的探索。
Ann-Shyn Chiang Ph. D. 江安世 教授
Tsing Hua Distinguished Chair Professor 國立清華大學特聘講座教授
Director, Brain Research Center 國立清華大學腦科學研究中心主任
National Tsing Hua University, Hsinchu 300044, Taiwan 終身國家講座, 中央研究院院士, 世界科學院院士
連絡電話: 886-3-5742760
International Faculty, Kavli Institute for Brain and Mind (KIBM) at the University of California, San Diego 美國聖地牙哥 Kavli腦與心智研究中心國際研究
連絡電郵: a6chiang@ucsd.edu
代表著作
Stampfl APJ, Liu Z, Hu J, Sawada K, Takano H, Kohmura Y, Ishikawa T, Lim JH, Je JH, Low CM, Teo A, Tok ES, Tan TW, Ban K, Libedinsky C, Tan FCK, Chen KP, Yang AC, Chuang CC, Chen NY, Shih CT, Lee TK, Yang DN, Lai HC, Shuai HH, Cheng CC, Ching YT, Li CW, Charng CC, Lo CC, Chiang AS, Recur B, Petibois C, Cheng CL, Chen HH, Yang SM, Hwu Y, Rojviriya C, Rugmai S, Rujirawat S, Margaritondo G* (2023) SYNAPSE: An international roadmap to large brain imaging. Physics Reports 999, 1–60. (IF: 25.6) [DOI]
Lin HW, Chen CC*, Jhang RY, Chen L, de Belle JS, Tully T, Chiang AS* (2022) CREBB repression of protein synthesis in mushroom body gates long-term memory formation in Drosophila. Proc Natl Acad Sci USA 119, e2211308119. (IF: 12.779)
Lin HW#, Chen CC#, de Belle JS, Tully T*, Chiang AS* (2021) CREBA and CREBB in two identified neurons gate long-term memory formation in Drosophila. Proc. Natl. Acad. Sci. U.S.A. 118, ppe2100624118. (IF: 11.205) [DOI]
Chu LA# Lu CH#, Yang SM, Liu YT, Feng KL, Tsai YCh, Chang WK, Wang WC, Chang SW, Chen P, Lee TK, Hwu YK, Chiang AS*, Chen BC* (2019) Rapid single-wavelength lightsheet localization microscopy for clarified tissue. Nature Communications 10, 4762. (IF: 14.919) [DOI]
Feng KL and Chiang AS* (2019) Forgetting memories through distinct actin remodeling mechanisms Proc. Natl. Acad. Sci. U.S.A. 116, 20807–20808. (IF: 11.205)
Sung YC, Jin PR, Chu LA, Hsu FF, Wang MR, Chang CC, Chiou SJ, Qiu JT, Gao DY, Lin CC, Chen YS, Hsu YC, Wang J, Wang FN, Yu PL, Chiang AS, Wu Anthony YT, Ko John JS, Lai Charles PK, Lu TT*, Chen Y* (2019) Delivery of nitric oxide with a nanocarrier promotes tumour vessel normalization and potentiates anti-cancer therapies. Nature Nanotechnology 14, 1160-1169. (IF: 39.213)
Zhang Y, Tsang TK, Bushong EA, Chu LA, Chiang AS, Ellisman MH, Reingruber J, Su CY* (2019) Asymmetric ephaptic inhibition between compartmentalized olfactory receptor neurons. Nature Communications 10, 1560. (IF: 14.919)
Shih HW, Wu CL, Chang SW, Liu TH, Lai JSY, Fu TF, Fu CC, Chiang AS* (2015) Parallel circuits control temperature preference in Drosophila during aging. Nature Communications 6, 7775. (IF:14.919)
Shih CT*, Sporns O, Yuan SL, Su TS, Lin YJ, Chuang CC, Wang TY, Lo CC, Greenspan RJ, Chiang AS* (2015) Connectomics-based analysis of information flow in the Drosophila brain. Current Biology 25, 1249-1258. (IF: 10.834)
Wu MC, Chu LA, Hsiao PY, Lin YY, Chi CC, Liu TH, Fu CC*, Chiang AS* (2014) Optogenetic control of selective neural activity inmultiple freely moving Drosophila adults. Proc. Natl. Acad. Sci. U.S.A. 111, 5367-5372. (IF: 11.205)
Wu CL, Shih MF M, Lee PT, Chiang AS* (2013) An octopamine–mushroom body circuit modulates the formation of anesthesia-resistant memory in Drosophila. Current Biology 23, 1-9. (IF: 10.834)
Lin HH, Chu LA, Fu TF, Dickson BJ, Chiang AS* (2013) Parallel neural pathways mediate CO2 avoidance responses in Drosophila. Science 340, 1338-1341. (IF: 47.728; this article has been reviewed in the “PERSPECTIVES” of the same Science issue, p1295-1297)
Pai TP, Chen CC, Lin HH, Chin AL, Lai JSY, Lee PT, Tully T, Chiang AS* (2013) Drosophila ORB protein in two mushroom body-output neurons is necessary for long-term memory formation. Proc. Natl. Acad. Sci. U.S.A. 110, 7898-7903. (IF: 11.205)
Lehnert BP, Baker AE, Gaudry Q, Chiang AS, Wilson RI* (2013) Distinct roles of TRP channels in auditory transduction and amplification in Drosophila. Neuron 77, 115-128. (IF: 15.982)
Chen CC, Wu JK, Lin HW, Pai TP, Fu TF, Wu CL, Tully T, Chiang AS* (2012) Visualizing long-term memory formation in two neurons of the Drosophila brain. Science 335, 678–685. [101年大學指考試題39-41] (IF: 47.728, cited by 99 articles; this article has been reviewed in the “PERSPECTIVES” of the same Science issue, p664-665; this article has been introduced by the Science Editor in the “Editor’s choice” of the Science Signaling 14, ec50)
Lai JSY, Lo SJ, Dickson BJ and Chiang AS* (2012) Auditory circuit in the Drosophila brain. Proc. Natl. Acad. Sci. U.S.A. 109, 2607-2612. (IF: 11.205)
Chiang AS* (2011) Q&A: Ann-Shyn Chiang. Current Biology 21, R938-R939. (IF: 10.834)
Lee PT, Lin HW, Chang YH, Fu TF, Dubnau J, Hirsh J, Lee T and Chiang AS* (2011) Serotonin-mushroom body circuit modulating the formation of anesthesia-resistant memory in Drosophila. Proc. Natl. Acad. Sci. U.S.A. 108, 13794–13799. (IF: 11.205)
Wu CL, Shih MF Lai SY, Yang HT, Turner GC, Chen L, Chiang AS* (2011) Heterotypic gap junctions between two neurons in the Drosophila brain are critical for memory. Current Biology 21, 848-854. (IF: 8.983; this article has been reviewed in the “Dispatch” of the Current Biology 21, R394-R395)
Chiang AS*, Lin CY, Chuang CC, Chang HM, Hsieh CH, Yeh CW, Shih CT, Wu JJ, Wang GT, Chen YC, Wu CC, Chen GY, Ching YT, Lee PC, Lin CY, Lin HH, Wu CC, Hsu HW, Huang YA, Chen JY, Chiang HJ, Lu CF, Ni RF, Yeh CY, Hwang JK (2011) Three-dimensional reconstruction of brainwide wiring networks in Drosophila at single cell resolution. Current Biology 21,1-11. (Article, cover story) (IF: 10.834; cited by 325 articles; this article has been reviewed in the “Dispatch” of the Current Biology 21, R19-R20)「紐約時報」專欄報導
Wu CL, Xia S, Fu TF, Wang H, Chen YH, Leong D, Chiang AS*, Tully T* (2007) Specific requirement of NMDA receptors for long-term memory consolidation in Drosophila ellipsoid body. Nature Neuroscience 10, 1578-1586. (IF: 24.884)
Lin HH, Lai JSY, Chin AL, Chen YC, Chiang AS* (2007) A map of olfactory representation in the Drosophila mushroom body. Cell 128, 1205-1218. (IF: 41.582)
Zhu S, Lin S, Kao CF, Awasaki T, Chiang AS, Lee T* (2006) Gradients of the Drosophila chinmo BTB-zinc finger protein govern neuronal temporal identity. Cell 127, 409-422. (IF: 41.582)
Xia S, Miyashita T, Fu TF, Lin WY, Wu CL, Pyzocha L, Lin IR, Saitoe M, Tully T, Chiang AS* (2005) NMDA receptors mediate olfactory learning and memory in Drosophila. Current Biology 15, 603-615. (IF: 10.834; this article has been reviewed in the “Dispatch” of the Current Biology 15, R414-R419)
Agrawal N, Pallos J, Slepko N, Apostol BL, Bodai L, Chang LW, Chiang AS, Thompson LM, Marsh JL (2005) Identification of combinatorial drug regimens for treatment of Huntington’s disease using Drosophila. Proc. Natl. Acad. Sci. U.S.A. 102, 3777-3781. (IF: 11.205)
Iijima K, Liu HP, Chiang AS, Konsolaki M, Zhong Y* (2004) Dissecting the pathological effects of human Abeta40 and Abeta42 in Drosophila: a potential model for Alzheimer's disease. Proc. Natl. Acad. Sci. U.S.A. 101, 6623-6628. (IF: 11.205)
Tamura T, Chiang AS, Ito N, Liu HP, Horiuchi J, Tully T, Saitoe M* (2003) Aging specifically impairs amnesiac-dependent memory in Drosophila. Neuron 40, 1003-1011. (IF: 15.982)
Chiang AS*, Lin WY, Liu HP, Pszczolkowski MA, Fu TF, Chiu SL, Holbrook GL (2002) Insect NMDA receptors mediate juvenile hormone biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 99, 37-42. (IF: 11.205)
