Drosophila Memory Connectome

Our interdisciplinary team is pioneering efforts to understand how the brain controls behavior and cognition. We are the first in the world to construct a 3D fly brain database at single-neuron resolution, a breakthrough that was featured in The New York Times article, "Decoding the Human Brain, With Help From a Fly."

Building on this achievement, we discovered that learning-induced new protein synthesis occurs in only a few brain neurons for the formation of long-term memory—research that was published in Science magazine. Our team remains dedicated to uncovering how pairing external odor information with punishment or reward shapes long-term memory in fruit flies.

本研究團隊於2010年12月發表了解析度到達單細胞層次之果蠅全腦網絡立體圖譜，獲刊於《現代生物學》雜誌，廣受國內外媒體報導，紐約時報更盛讚此研究為人類全腦圖譜解碼之第一步。藉由此圖譜，本團隊更發現為了現儲存長期記憶所引發的蛋白質新生，僅發生在果蠅腦中少數幾顆特定的神經細胞內，並成為國內首位以長篇論文形式發表在《科學》雜誌之研究。我們會持續針對果蠅長期記憶形成的分子機制以及嗅覺神經網路做更深入的探索。

Ann-Shyn Chiang Ph. D.   江安世 教授

• Tsing Hua Distinguished Chair Professor 國立清華大學特聘講座教授

• Director, Brain Research Center 國立清華大學腦科學研究中心主任

• National Tsing Hua University, Hsinchu 300044, Taiwan 終身國家講座, 中央研究院院士, 世界科學院院士

• 連絡電話: 886-3-5742760

• 連絡電郵: aschiang@life.nthu.edu.tw

• International Faculty, Kavli Institute for Brain and Mind (KIBM) at the University of California, San Diego 美國聖地牙哥 Kavli腦與心智研究中心國際研究

• 連絡電郵: a6chiang@ucsd.edu

• More About Leader

代表著作

1.  Chu LA, Tai CY, Chiang AS* (2024) Thirst-driven hygrosensory suppression promotes water seeking in Drosophila. Proc Natl Acad Sci USA 121, e2404454121.  (IF: 9.4)

2.  Chuang YH, Wu YF, Lin YH, Chen YH, Zhou YX, Hsu SC, Li SM, Chiang AS, Chen YC, Chen SJ, Lin SJ* and Chu LA* (2024) Super resolution imaging in collagen abundant thick tissues. Small Structures 2024, 2400231. (IF: 15.9).

3. Abubaker MB, Hsu FY, Feng KL, Chu LA, de Belle JS, Chiang AS* (2024) Asymmetric neurons are necessary for olfactory learning in the Drosophila brain. Current Biology 34, 1–12. (IF: 10.834) [DOI]

4. Chen CC*, Lin HW, Feng KL, Tseng DW, de Belle JS, Chiang AS* (2023) A subset of cholinergic mushroom body neurons blocks long-term memory formation in Drosophila. Cell Reports 42,112974. (IF: 9.995)

5. Sakamura S, Hsu FY, Tsujita A, Bin Abubaker M, Chiang AS, Matsuno K* (2023) Ecdysone signaling determines lateral polarity and remodels neurites to form Drosophila’s left-right brain asymmetry. Cell Reports 42, 112337. (IF: 9.995)

6. Manubens-Gil L, Zhou Z, Chen H, Ramanathan A, Liu X, Liu Y, Bria A, Gillette T, Ruan Z, Yang J, Radojević M, Zhao T, Cheng L, Qu L, Liu S, Bouchard K, Gu L, Cai W, Ji S, Roysam B, Wang CW, Yu H, Sironi A, Iascone DM, Zhou J, Bas E, Sousa EC, Aguiar P, Li X, Li Y, Nanda S, Wang Y, Muresan L, Fua P, Ye B, He H, Staiger JF, Peter M, Cox DN, Simonneau M, Oberlaender M, Jefferis G, Ito K, Bellido PG, Kim J, Rubel E, Cline HT, Zeng H, Nern A, Chiang AS, Roskams J, Livesey R, Stevens J, Liu T, Dang C, Guo Y, Zhong N, Tourassi G, Hill S, Hawrylycz M, Koch C, Meijering E*, Ascoli GA*, Peng H* (2023) BigNeuron: A resource to benchmark and predict performance of algorithms for automated tracing of neurons in light microscopy datasets. Nature Methods 20, 824-835. (IF: 47.99)

7. Stampfl APJ, Liu Z, Hu J, Sawada K, Takano H, Kohmura Y, Ishikawa T, Lim JH, Je JH, Low CM, Teo A, Tok ES, Tan TW, Ban K, Libedinsky C, Tan FCK, Chen KP, Yang AC, Chuang CC, Chen NY, Shih CT, Lee TK, Yang DN, Lai HC, Shuai HH, Cheng CC, Ching YT, Li CW, Charng CC, Lo CC, Chiang AS, Recur B, Petibois C, Cheng CL, Chen HH, Yang SM, Hwu Y, Rojviriya C, Rugmai S, Rujirawat S, Margaritondo G* (2023) SYNAPSE: An international roadmap to large brain imaging. Physics Reports 999, 1–60. (IF: 25.6) [DOI]

8. Lin HW, Chen CC*, Jhang RY, Chen L, de Belle JS, Tully T, Chiang AS* (2022) CREBB repression of protein synthesis in mushroom body gates long-term memory formation in Drosophila. Proc Natl Acad Sci USA 119, e2211308119. (IF: 12.779) 

9. Lin HW#, Chen CC#, de Belle JS, Tully T*, Chiang AS*  (2021) CREBA and CREBB in two identified neurons gate long-term memory formation in Drosophila. Proc. Natl. Acad. Sci. U.S.A. 118, ppe2100624118. (IF: 11.205)  [DOI]

10. Chu LA# Lu CH#, Yang SM, Liu YT, Feng KL, Tsai YCh, Chang WK, Wang WC, Chang SW, Chen P, Lee TK, Hwu YK, Chiang AS*, Chen BC* (2019) Rapid single-wavelength lightsheet localization microscopy for clarified tissue. Nature Communications 10, 4762. (IF: 14.919) [DOI]

11. Feng KL and Chiang AS* (2019) Forgetting memories through distinct actin remodeling mechanisms Proc. Natl. Acad. Sci. U.S.A. 116, 20807–20808. (IF: 11.205)

12. Sung YC, Jin PR, Chu LA, Hsu FF, Wang MR, Chang CC, Chiou SJ, Qiu JT, Gao DY, Lin CC, Chen YS, Hsu YC, Wang J, Wang FN, Yu PL, Chiang AS, Wu Anthony YT, Ko John JS, Lai Charles PK, Lu TT*, Chen Y*  (2019) Delivery of nitric oxide with a nanocarrier promotes tumour vessel normalization and potentiates anti-cancer therapies. Nature Nanotechnology 14, 1160-1169. (IF: 39.213)

13. Zhang Y, Tsang TK, Bushong EA, Chu LA, Chiang AS, Ellisman MH, Reingruber J, Su CY* (2019) Asymmetric ephaptic inhibition between compartmentalized olfactory receptor neurons. Nature Communications 10, 1560. (IF: 14.919)

14. Shih HW, Wu CL, Chang SW, Liu TH, Lai JSY, Fu TF, Fu CC, Chiang AS* (2015) Parallel circuits control temperature preference in Drosophila during aging. Nature Communications 6, 7775. (IF:14.919)

15. Shih CT*, Sporns O, Yuan SL, Su TS, Lin YJ, Chuang CC, Wang TY, Lo CC, Greenspan RJ, Chiang AS* (2015) Connectomics-based analysis of information flow in the Drosophila brain. Current Biology 25, 1249-1258. (IF: 10.834)

16. Wu MC, Chu LA, Hsiao PY, Lin YY, Chi CC, Liu TH, Fu CC*, Chiang AS* (2014) Optogenetic control of selective neural activity inmultiple freely moving Drosophila adults. Proc. Natl. Acad. Sci. U.S.A. 111, 5367-5372. (IF: 11.205)

17. Wu CL, Shih MF M, Lee PT, Chiang AS* (2013) An octopamine–mushroom body circuit modulates the formation of anesthesia-resistant memory in Drosophila. Current Biology 23, 1-9. (IF: 10.834)

18. Lin HH, Chu LA, Fu TF, Dickson BJ, Chiang AS* (2013) Parallel neural pathways mediate CO2 avoidance responses in Drosophila. Science 340, 1338-1341. (IF: 47.728; this article has been reviewed in the “PERSPECTIVES” of the same Science issue, p1295-1297)

19. Pai TP, Chen CC, Lin HH, Chin AL, Lai JSY, Lee PT, Tully T, Chiang AS* (2013) Drosophila ORB protein in two mushroom body-output neurons is necessary for long-term memory formation. Proc. Natl. Acad. Sci. U.S.A. 110, 7898-7903. (IF: 11.205)

20. Lehnert BP, Baker AE, Gaudry Q, Chiang AS, Wilson RI* (2013) Distinct roles of TRP channels in auditory transduction and amplification in Drosophila. Neuron 77, 115-128. (IF: 15.982)

21. Chen CC, Wu JK, Lin HW, Pai TP, Fu TF, Wu CL, Tully T, Chiang AS* (2012) Visualizing long-term memory formation in two neurons of the Drosophila brain. Science 335, 678–685. [101年大學指考試題39-41] (IF: 47.728, cited by 99 articles; this article has been reviewed in the “PERSPECTIVES” of the same Science issue, p664-665; this article has been introduced by the Science Editor in the “Editor’s choice” of the Science Signaling 14, ec50)

22. Lai JSY, Lo SJ, Dickson BJ and Chiang AS* (2012) Auditory circuit in the Drosophila brain. Proc. Natl. Acad. Sci. U.S.A. 109, 2607-2612. (IF: 11.205)

23. Chiang AS* (2011) Q&A: Ann-Shyn Chiang. Current Biology 21, R938-R939. (IF: 10.834)

24. Lee PT, Lin HW, Chang YH, Fu TF, Dubnau J, Hirsh J, Lee T and Chiang AS* (2011) Serotonin-mushroom body circuit modulating the formation of anesthesia-resistant memory in Drosophila. Proc. Natl. Acad. Sci. U.S.A. 108, 13794–13799. (IF: 11.205)

25. Wu CL, Shih MF Lai SY, Yang HT, Turner GC, Chen L, Chiang AS* (2011) Heterotypic gap junctions between two neurons in the Drosophila brain are critical for memory. Current Biology 21, 848-854. (IF: 8.983; this article has been reviewed in the “Dispatch” of the Current Biology 21, R394-R395)

26. Chiang AS*, Lin CY, Chuang CC, Chang HM, Hsieh CH, Yeh CW, Shih CT, Wu JJ, Wang GT, Chen YC, Wu CC, Chen GY, Ching YT, Lee PC, Lin CY, Lin HH, Wu CC, Hsu HW, Huang YA, Chen JY, Chiang HJ, Lu CF, Ni RF, Yeh CY, Hwang JK (2011) Three-dimensional reconstruction of brainwide wiring networks in Drosophila at single cell resolution. Current Biology 21,1-11. (Article, cover story) (IF: 10.834; cited by 325 articles; this article has been reviewed in the “Dispatch” of the Current Biology 21, R19-R20)「紐約時報」專欄報導

27. Wu CL, Xia S, Fu TF, Wang H, Chen YH, Leong D, Chiang AS*, Tully T* (2007) Specific requirement of NMDA receptors for long-term memory consolidation in Drosophila ellipsoid body. Nature Neuroscience 10, 1578-1586. (IF: 24.884)

28. Lin HH, Lai JSY, Chin AL, Chen YC, Chiang AS* (2007) A map of olfactory representation in the Drosophila mushroom body. Cell 128, 1205-1218. (IF: 41.582)

29. Zhu S, Lin S, Kao CF, Awasaki T, Chiang AS, Lee T* (2006) Gradients of the Drosophila chinmo BTB-zinc finger protein govern neuronal temporal identity. Cell 127, 409-422. (IF: 41.582)

30. Xia S, Miyashita T, Fu TF, Lin WY, Wu CL, Pyzocha L, Lin IR, Saitoe M, Tully T, Chiang AS* (2005) NMDA receptors mediate olfactory learning and memory in Drosophila. Current Biology 15, 603-615. (IF: 10.834; this article has been reviewed in the “Dispatch” of the Current Biology 15, R414-R419)

31. Agrawal N, Pallos J, Slepko N, Apostol BL, Bodai L, Chang LW, Chiang AS, Thompson LM, Marsh JL (2005) Identification of combinatorial drug regimens for treatment of Huntington’s disease using Drosophila. Proc. Natl. Acad. Sci. U.S.A. 102, 3777-3781. (IF: 11.205)

32. Iijima K, Liu HP, Chiang AS, Konsolaki M, Zhong Y* (2004) Dissecting the pathological effects of human Abeta40 and Abeta42 in Drosophila: a potential model for Alzheimer's disease. Proc. Natl. Acad. Sci. U.S.A. 101, 6623-6628. (IF: 11.205)

33. Tamura T, Chiang AS, Ito N, Liu HP, Horiuchi J, Tully T, Saitoe M* (2003) Aging specifically impairs amnesiac-dependent memory in Drosophila. Neuron 40, 1003-1011. (IF: 15.982)

34. Chiang AS*, Lin WY, Liu HP, Pszczolkowski MA, Fu TF, Chiu SL, Holbrook GL (2002) Insect NMDA receptors mediate juvenile hormone biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 99, 37-42. (IF: 11.205)