FACS Canto II

BD FACSCantoTMII: 

It is configured with 3 lasers and 8 fluorescence parameters. 

Windows XP operating system with FACS DivaTM Software for acquisition & analysis

Laser Specifications: Air-cooled, 20 mW solid state 488 nm, 17 mW HeNe 633 nm laser and 10mW-17mW Point Source Violet solid state laser 405nm 

Optics configurations: 

FSC Detector Photodiode with 488/10BP, 

SSC Detector PMT with 488/10BP

Fluorescence Detectors: 8 PMTs in 4+2+2 configuration

Four wavelengths detected from the 488-nm laser (standard filters): (1).  515-545 nm FITC  (2). 564-606 nm PE  (3).  670LP nm PE-Cy5 or PerCP (4).  750-810 nm PE-Cy7

Two wavelengths detected from the 633-nm laser (standard filters): (1). 650-670 nm APC (2). 750-810 nm APC-Cy7

Two wavelengths detected from the 405-nm laser (standard filters): (1). 485-535 nm AmCyan (2).  425-475 nm Pacific Blue 

Sample Injection: Direct into flow cell

Max Particle Size: 50 μm

Flow Rate, min: 10 μL/min

Flow Rate, max: 120 μL/min

Sample Acquisition Rate: 10,000 events/sec,

10 channels with 8 compensated fluorescent parameters

Nominal Minimum Sample Volume: 30 μL