CytoFLEX LX

5 High Power Lasers 488nm, 638nm, 405nm, 561nm and 355nm with 19 Color & 21 Parameters.

3 fluorescent channels from the 355 nm (UV) laser,

5 from the 405 nm (Violet) laser,

3 from the 488 nm (Blue) laser,

5 from the 561 nm (Yellow Green) laser,

3 from the 638 nm (Red) laser,

(Also, details of the laser and filter configurations have been provided below)

PERFORMANCE

SCATTER RESOLUTION

Blue (488 nm) Side Scatter Resolution: <300 nm

Violet (405 nm) Side Scatter Resolution (VSSC): 80 nm polystyrene particles

Scatter performance is optimized for resolving human lymphocytes, monocytes, and granulocytes as well as nanoparticles.

CARRYOVER

Single Tube Format: ≤ 1.0%

Plate Loader Format: < 0.5%

SENSITIVITY

FITC: <30 molecules of equivalent soluble fluorochrome (MESF-FITC) from the 488 nm laser.

PE: <10 molecules of equivalent soluble fluorochrome (MESF-PE) from the 488 nm laser.

FLUORESCENCE RESOLUTION

The CytoFLEX Flow Cytometer is capable of achieving <3% rCV with alignment verification particles capable of rCVs <3%.

ELECTRONICS

NOMINAL ACQUISITION RATE

30,000 events per second with all configured parameters

Software capability to modify window extension parameter and to control abort rate during high event rate signal processing

SIGNAL PROCESSING

Fully digital system with 7 decade data display

SIGNAL

Pulse area, height for every channel, width for one selectable channel

AUTOMATED MAINTENANCE FUNCTIONS

System Startup, Sample Mixing, Backflush, Prime, Shutdown, Deep Clean

SAMPLE INPUT FORMATS

5 mL (12 x 75 mm) polystyrene and polypropylene tubes

1.5 mL and 2 mL microcentrifuge tubes

PLATE LOADER FORMATS

96-well Standard Flat, U and V bottom plates, and 96-deep well plates.

FLUIDICS

Ultra-low pressure peristaltic sheath and sample delivery system

Sheath Fluid Filter and Sample Pump Tubing can be replaced by the user

(no service visit required)

SAMPLE FLOW RATES

Fixed Flow Rates: 10, 30 and 60 μL/min

Custom Flow Rate Control mode from 10 to 240 μL/min in 1 μL increments.

Details Specifications/Configurations in Technical Notes:

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