CytoFLEX LX
CytoFLEX LX
5 High Power Lasers 488nm, 638nm, 405nm, 561nm and 355nm with 19 Color & 21 Parameters.
3 fluorescent channels from the 355 nm (UV) laser,
5 from the 405 nm (Violet) laser,
3 from the 488 nm (Blue) laser,
5 from the 561 nm (Yellow Green) laser,
3 from the 638 nm (Red) laser,
(Also, details of the laser and filter configurations have been provided below)
PERFORMANCE
SCATTER RESOLUTION
Blue (488 nm) Side Scatter Resolution: <300 nm
Violet (405 nm) Side Scatter Resolution (VSSC): 80 nm polystyrene particles
Scatter performance is optimized for resolving human lymphocytes, monocytes, and granulocytes as well as nanoparticles.
CARRYOVER
Single Tube Format: ≤ 1.0%
Plate Loader Format: < 0.5%
SENSITIVITY
FITC: <30 molecules of equivalent soluble fluorochrome (MESF-FITC) from the 488 nm laser.
PE: <10 molecules of equivalent soluble fluorochrome (MESF-PE) from the 488 nm laser.
FLUORESCENCE RESOLUTION
The CytoFLEX Flow Cytometer is capable of achieving <3% rCV with alignment verification particles capable of rCVs <3%.
ELECTRONICS
NOMINAL ACQUISITION RATE
30,000 events per second with all configured parameters
Software capability to modify window extension parameter and to control abort rate during high event rate signal processing
SIGNAL PROCESSING
Fully digital system with 7 decade data display
SIGNAL
Pulse area, height for every channel, width for one selectable channel
AUTOMATED MAINTENANCE FUNCTIONS
System Startup, Sample Mixing, Backflush, Prime, Shutdown, Deep Clean
SAMPLE INPUT FORMATS
5 mL (12 x 75 mm) polystyrene and polypropylene tubes
1.5 mL and 2 mL microcentrifuge tubes
PLATE LOADER FORMATS
96-well Standard Flat, U and V bottom plates, and 96-deep well plates.
FLUIDICS
Ultra-low pressure peristaltic sheath and sample delivery system
Sheath Fluid Filter and Sample Pump Tubing can be replaced by the user
(no service visit required)
SAMPLE FLOW RATES
Fixed Flow Rates: 10, 30 and 60 μL/min
Custom Flow Rate Control mode from 10 to 240 μL/min in 1 μL increments.
Details Specifications/Configurations in Technical Notes: