FACS Calibur

BD FACSCalibur^TM:

It is dual laser with 4-color parameters. Mac operating system with CellQuestPro Software for acquisition & analysis.

Laser Specifications:15mW 488nm, air-cooled argon-ion laser; Red diode laser, 635nm

Fluorescence Detectors and Filters: High performance, high dynamic range photo- multipliers with bandpass filters : 530nm (FITC), 585nm (PE/PI), 661nm (APC) and >650nm (PerCP) with base unit, >670nm (PerCP) with FL4 option

Sample Flow rate: Three selectable flow rates of 60mL/min, 35mL/min and 12mL/min. Particle velocity in flow cell is approximately 6 meters/sec

Sample Concentration: Single-cell suspension of 10⁵ to 2 x 10⁷ particles/mL recommended range. Kindly use 12 x 75-mm polystyrene tubes (Falcon) (Part No. 54-10001-00) for samples for acquisition.