In the Make Your Mutant Lab under the instruction of Mario Lopez and Dr. Jaime Mayoral, we sought to characterize bacterial β-Glucosidase B (lovingly named BglB) by designing mutant bacterial variants of BglB in a 3D molecular protein folding prediction software, performing Kunkel Mutagenesis to bring these designs to fruition, and performing kinetic and thermostability assays on the mutants. BglB has millions upon millions of potential mutations which makes prediction of how the protein would fold with these mutations a difficult task. This research achieves two main goals: to characterize BglB, a crucial enzyme for many aspects of living organisms and life itself, and to improve protein folding prediction algorithms by affirming or denying folding predictions. This research was conducted on site Y396 of BglB with a mutation from a tyrosine to a proline. As expected, the protein did not express with the mutation.