The aim of this exercise is to determine the size of a DNA band from an agarose gel with the help of DNA-marker.
In the following you are provided with a picture of an agarose gel, which contains DNA-fragments of known sizes (white bands), some yellow bands and a red band (indicated with "X") of unknown size.
a) Construct a calibration (standard) curve for the known fragment sizes;
b) Determine the fragment size of the unknown red band;
c) Report your results in the appropriate way.
For the calibration curve measure the distance each fragment of known size has migrated away from the start point (the loading well). Plot these distances on the y-axis (dependent variable) versus the logarithm of the size of the known fragments on the x-axis (independent variable).
Measure the distance of the red (unknown size) band from the starting point (loading well). Use the calibration curve to determine the fragment size of the unknown band. Remember that the calibration curve provides you with the logarithm of the fragment size!