The aim of this exercise is to determine an unknown protein concentration using a Bradford Assay. The measurements of the absorbances of known protein concentrations will be used to generate a standard (=calibration) curve. With the help of this standard and the measurements of the absorbance of an unknown sample the concentration of the unknown sample can be determined.
Click on the "New"-button to start an experiment.
Click on the "Standards"-button and choose the concentration of the known sample you want to measure.
Click on the "Run"-button and record the absorbance.
Repeat steps 2 and 3 for all the standard concentrations. Do NOT click the "New"-button!
Click on the "Unknowns"-button - the measurement of the unknown sample will be performed in triplicates to enhance accuracy of the measurements. Select "Sample 1".
Click on the "Run"-button and record the absorbance of the unknown Sample 1.
Repeat steps 5 and 6 for Samples 2 and 3 of the unknown sample.
Produce a standard curve from the known concentrations and determine the mean of the unknown protein concentration.
If you want to analyse a new data set, please click on the "New"-button again and start another experiment.
Here is the link to a Youtube playlist that shows, how data from a standard curve can be analysed.