CRISPR-Cas Technologies

CRISPR-Cas Technologies 

Our laboratory is dedicated to advancing CRISPR-based genome editing technologies in microbial model systems. We focus on the development of single-nucleotide–resolution editing, multiplex genome manipulation, and CRISPR interference (CRISPRi) platforms to precisely regulate and reprogram cellular functions. 

- Selected Publications -

• • Lee HJ, Kim HJ, Lee SJ. CRISPR-Cas9-mediated pinpoint microbial genome editing aided by target-mismatched sgRNAs. Genome Research (2020). PubMed PMID: 32327447

  • Lee HJ, Kim HJ, Lee SJ. Miniature CRISPR-Cas12f1-mediated single-nucleotide microbial genome editing using 3′-truncated sgRNA. CRISPR Journal (2023). PubMed PMID: 36576897

  • Jeong SH, Lee HJ, Lee SJ. Use of paired Cas9-NG nickase and truncated sgRNAs for single-nucleotide microbial genome editing. Frontiers in Genome Editing (2024). PubMed PMID: 39391173