Embedded Files
Introduction
Introduction
There is a substance called a catalyst which increases the speed of a chemical reaction without changing the actual reaction. When dealing with organisms, the catalyst used for those reactions is called an enzyme. Enzymes are commonly proteins but they can be seen in different forms such as ribonucleic acid molecules.
There is a substance called a catalyst which increases the speed of a chemical reaction without changing the actual reaction. When dealing with organisms, the catalyst used for those reactions is called an enzyme. Enzymes are commonly proteins but they can be seen in different forms such as ribonucleic acid molecules.
Enzymes are proteins which serve as a catalyst by reducing the amount of energy required to perform chemical reactions. This speeds up the rate at which chemical reactions can occur. Without enzymes chemical reactions would not occur fast enough to continue life. Enzymes tend to be placed in pH levels which are similar to the human body, enabling common enzymes in the human body such as amylase to function in their optimum condition.
Enzymes are proteins which serve as a catalyst by reducing the amount of energy required to perform chemical reactions. This speeds up the rate at which chemical reactions can occur. Without enzymes chemical reactions would not occur fast enough to continue life. Enzymes tend to be placed in pH levels which are similar to the human body, enabling common enzymes in the human body such as amylase to function in their optimum condition.
Carrots have an enzyme known as catalyst, which converts hydrogen catalyst into water and oxygen. Catalyst is affected by the pH level of its surroundings, and works best in an optimum pH level. The optimum pH level of catalyst is in the range of pH levels of 5-7.
Carrots have an enzyme known as catalyst, which converts hydrogen catalyst into water and oxygen. Catalyst is affected by the pH level of its surroundings, and works best in an optimum pH level. The optimum pH level of catalyst is in the range of pH levels of 5-7.
Objective
Objective
What pH level will be the most suitably for catalase in carrots to catalyze hydrogen peroxide and achieve optimum levels.
What pH level will be the most suitably for catalase in carrots to catalyze hydrogen peroxide and achieve optimum levels.
Materials
Materials
- 4 Test Tubes
- Hydrogen Peroxide
- Test Tube Rack
- Dropper Pipet
- Vinger
- Spatula
- Graduated Cylinder
- Labeling Tape
- Permanent Maker
- Digital Scale
- Glass Mixing Rod
- Water
- Grater
- 3 Beaker
- Paper Plate
- Test Tube scrubber
- Weighing Dish
- Ruler
- pH Strips
Procedures
Procedures
- Gather the materials
- Create the solutions with different ratios of vinegar and water. Place 50 mL of pure vinegar (3 pH), 50 mL of a ratio of 1:10 of vinegar to water (4 pH), 50 mL of a ratio of a 1:20 of vinegar to water (5 pH), and pure water (7 pH) in different beakers. Test the pH levels by using pH test strips.
- Shed the carrots into small carrots pieces using the grader
- Place on the Paper Plate
4. Place test tubes into test tube rack
- Use the tape & permanent marker to label test tubes 1-4
5. Grab the digital scale
- Turn it on
- Place Weighing dish on scale and zero it
6. Measure 2 Grams of carrots using the spatula
- Place 2 grams into each test tube using the glass mixing rod to push down the carrots
7. Put 20 drops of the vinegar into test tube 1-3 using the dropper pipet
- Test Tube 1: 20 drops of pH 3
- Test Tube 2: 20 drops of pH 4
- Test Tube 3: 20 drops of pH 5
8. Put 20 drops of water into test tube 4
9. Put 3 ml of Hydrogen Peroxide into each test tube using the graduated cylinder
10. Measure how high the bubbles go using the ruler
11. Clean up the materials
- Be sure to scrub the test tubes with the test tube scrubber
12. Write the lab report
Safety
Safety
- Report all accidents, injuries, and breakage of glass or equipment to instructor immediately.
- Keep pathways clear by placing extra items (books, bags, etc.) on the shelves or under the work tables. If under the tables, make sure that these items can not be stepped on.
- Long hair (chin-length or longer) must be tied back to avoid catching fire.
- Wear sensible clothing including footwear. Loose clothing should be secured so they do not get caught in a flame or chemicals.
- Work quietly — know what you are doing by reading the assigned experiment before you start to work. Pay close attention to any cautions described in the laboratory exercises
- Do not taste or smell chemicals.
- Wear safety goggles to protect your eyes when heating substances, dissecting, etc.
- Do not attempt to change the position of glass tubing in a stopper.
- Never point a test tube being heated at another student or yourself. Never look into a test tube while you are heating it.
- Unauthorized experiments or procedures must not be attempted.
- Keep solids out of the sink.
- Leave your work station clean and in good order before leaving the laboratory.
- Do not lean, hang over or sit on the laboratory tables.
- Do not leave your assigned laboratory station without permission of the teacher.
- Learn the location of the fire extinguisher, eye wash station, first aid kit and safety shower.
- Fooling around or "horse play" in the laboratory is absolutely forbidden. Students found in violation of this safety rule will be barred from participating in future labs and could result in suspension.
- Anyone wearing acrylic nails will not be allowed to work with matches, lighted splints, bunsen burners, etc.
- Do not lift any solutions, glassware or other types of apparatus above eye level.
- Follow all instructions given by your teacher.
- Learn how to transport all materials and equipment safely.
- No eating or drinking in the lab at any time!
Pictures
Pictures
Google Sites
Report abuse