HIV-1 Research
HIV-1 trafficking on microtubules and nuclear import
During infection, HIV-1 engages the microtubule network of the host cell to deliver itself to the nuclear pore complex (NPC), through which the viral genome and other viral proteins enter the nucleus during infection. Our lab is interested in understanding how HIV-1 interacts with microtubule motors and other cytoskeletal proteins during infection with the hope of identifying interactions that might be disrupted in therapeutic strategies. We also want to know how the virus manages to enter the nucleus of a target cell while avoiding innate immune sensors and restriction factors designed to detect viruses like HIV-1 and prevent them from infecting the cell.
Campbell and Hope, Nat Rev Microbiology, 2015
Dharan A, Campbell EM. Teaching old dogmas new tricks: recent insights in the the nuclear import of HIV-1. Current Opinions in Virology, 2022 Feb 1; 53:101203
Neil SJD, Campbell EM. Fake Science: XMRV, COVID-1 and the Toxic Legacy of Dr. Judy Mikovits. AIDS Res Hum Retroviruses, 2020, Jul;36(7):545-549
Dharan A, Bachmann N, Talley S, Zwikelmaier V, Campbell, E. M. Nuclear Pore Blockade Reveals That HIV-1 Completes Reverse Transcription and Uncoating in the Nucleus. Nature Microbiology 2020, Sep 5(9):1088-1095
Adarsh Dharan and Edward M. Campbell, The Role of Microtubules and Microtubule Associated Proteins in HIV-1 Infection. Journal of Virology, 2018.
Adarsh Dharan, Silvana Opp, Omar Abdel-Rahim , Sevnur Komurlu Keceli , Sabrina Imam, Felipe Diaz-Griffero, Edward M. Campbell. Bicaudal D2 facilitates the cytoplasmic trafficking and nuclear import of HIV-1 genomes during infection, PNAS, 2017, Dec 12; 114(50)E10707
Dharan A, Talley S, Tripathi A, Mamede JI, Majetschak M, Hope TJ, Campbell EM. KIF5B and Nup358 Cooperatively Mediate the Nuclear Import of HIV-1 during Infection. PLoS Pathog. 2016 Jun; 12 (6)e1005700
Lukic Z, Dharan A, Fricke T, Diaz-Griffero F, Campbell EM. HIV-1 Uncoating is Facilitated by Dynein and Kinesin-1 J Virol. 2014 Sep 17. pii: JVI.02219-14.