Biosensor

Nuclear factor kappa B (NF-κB) regulates expression of several genes that mediate the inflammatory responses and cell proliferation, and is one of the therapeutic targets for chronic inflammatory disease and cancer. A novel molecular binding scheme for the detection of NF-κB was evaluated by investigating its affinity to the Ig-κB DNA composed by dye and quencher fluorophores, and this specificity was confirmed by competing with DNA sequence complementary to the Ig-κB DNA. The experiment of Hela nuclear extract showed that TNF-α stimulated Hela nuclear extract has higher affinity to Ig-κB DNA than non-TNF stimulated Hela nuclear extract (4-hour serum response). This design of biosensor is continued with advanced MEMS techniques.

1. Zhaohui Wang, Pak Kin Wong, “Development of DNA pair biosensor for quantization of nuclear factor Kappa B,” Biosensors, vol. 8(4), 126, 2018.

2. Zhaohui Wang, V. Gidwani, D. D. Zhang, P. K. Wong. “Separation-free detection of nuclear factor kappa B with double-stranded molecular probes,” Analyst 133, 998-1000 (2008).

3. Zhaohui Wang, V. Gidwani, Z. Sun, D. D. Zhang, P. K. Wong. “Development of a molecular assay for rapid screening of chemopreventive compounds targeting Nrf2,” Journal of the Association for Laboratory Automation 13, 243-248 (2008).

4. D. Meserve, Zhaohui Wang, D. D. Zhang, and P. K. Wong. “A double-stranded molecular probe for homogeneous nucleic acid analysis,” Analyst 133, 1313-1319 (2008).