Imaging 3D tumor systems

Video S1 Single cell analysis of MDA-MB-231 in 2D cultured conditions.mp4

Single-cell analysis of MDA-MB-231 cells grown in 2D monolayer cultured conditions

3D rendering of mitochondria using Tom20 immunostaining and Airyscan microscopy, followed by 3D rendering using "surface" module Imaris software (Wang et al., iScience, 2023).

Video S2 Single cell analysis of MDA-MB-231 in 3D cultured conditions.mp4

Single-cell analysis of MDA-MB-231 cells grown in 3D tumor liquid overlay tumor spheroids ultured conditions

3D rendering of mitochondria using Tom20 immunostaining and Airyscan microscopy, followed by 3D rendering using "surface" module Imaris software (Wang et al., iScience, 2023)

MDA231 3D cell volume.mp4

Role of 3D cancer cell growth on organelle morphology

3D rendering of cell volume and mitochondrial network of MDA-MB-231 breast cancer cell grown in 3D tumor spheroids (Wang et al., iScience, 2023).

microcapsule_z3.wmv

Heterogeneity of Transferrin uptake into 3D bioprinted breast tumor systems

3D rendering of transferrin-containing endosomes in MDA-MB-231 cells in 3D bioprinted microcapsules (Kingsley et al, 2019).

In collaboration with Dr. Xavier Intes, an optical imaging expert, Dr. David Corr, a 3D bioprinting expert, and Dr. John Lamar, an expert in tumor biology, we are developing new imaging methods to measure and assess heterogeneity of receptor-ligand binding (target engagement) and organelle morphology in 3D spheroids and 3D bioprinted breast tumor systems.

Barroso, Corr and Intes winter lunch (2018). From left to right: Denzel, Marien, Xavier, Jason, Alena, Margarida, Jamie, Ling, David, Kate and David.

David Corr, Margarida Barroso and Xavier Intes (2017)

Barroso, Corr and Intes group lab meeting (2018)