Embedded Files
Cancer Research, Cell Signaling, Molecular Biology, Biotechnology
Research Project-1: Tumor micro-environment: Tumor-associated macrophage
Research Project-1: Tumor micro-environment: Tumor-associated macrophage
Research Project-2: Moesin-PKM2 interaction in the TME
Research Project-2: Moesin-PKM2 interaction in the TME
Research Project-3: Osteoclastogenesis through GTP-RhoA pathway
Research Project-3: Osteoclastogenesis through GTP-RhoA pathway
Research Project-4: PD-L1 expression by cross-talking canonical and non-canonical TGF-β1 signaling in cancer
Research Project-4: PD-L1 expression by cross-talking canonical and non-canonical TGF-β1 signaling in cancer
Cloning
CTSL Gene Cloning
Cloning is an Important technique of molecular biology
I have experience in...
Gene(CDS) cloning using mammalian source as well as commercially available insert to prepare overexpressing plasmid DNA construct or fusion protein induction and purification, etc.
Domain cloning, to see the binding site of a protein in an specific region.
Promoter cloning to see the luciferase activity.
Western-blot
Protein Expression
Protein expression and Protein-Protein Interaction
I have experience in...
IP & co-IP
Kinase Assay/Protein Phosphorylation
Pull-down/in vitro binding assay
Immunostaining
Fluorescence & Confocal Microscope
Protein Localization and Protein-Protein Interaction
I have experience in...
Immunofluorescence Assay (Cell)
PLA assay (Cell)
H&E staining (Tissue)
ChIP-PCR
Chromatin Immunoprecepitation (ChIP) PCR
Protein-DNA interaction
I have experience in...
NLS, or protein complex-based nuclear localization, and their effects on specific gene transcription.
Cell-based experiment.
In-Vivo Study
Xenograft Experiment
Transplant of Tissue or Cells in BALB/c Mouse
I have experience in...
Handelin Mouse
Injection, regular observation, data collection, etc.
Technical Skills
Technical Skills
Cell Culture:
Cell line (RAW264, 4T1, BV2, MCF-7, MDA-MB-231, A549, Hep-G2, HEK293, etc.)
Primary cell (human lymphocyte, mouse monocyte, BMM, and PBM)
Bacterial strain: E. coli (DH5α and BL21), and
Fungi (Nigrospora oryzae, Colletotrichum siamense, etc.).
Analytical Assay:
UV and IR Spectrophotometry, MTT/CCK-8, Wound Healing, transwell migration and invasion, Western Blotting, IP, Co-IP, ChIP PCR, Pulldown assay, In vitro kinase assay, FACS assay, Immunofluorescence Assay, etc.
Microscopy:
Light Microscopy, Fluorescence Microscopy, and Confocal Microscopy.
Basic Bioinformatics:
NCBI, UCSC, Mega-Align, Sequence analysis, Primer design for PCR, Cloning, Point mutation, ChIP, qRT-PCR, etc. String, Gene, and protein structure prediction tools, etc.
Molecular Biology:
Molecular Cloning, Point mutation, Protein induction, purification, and Thrombin cleavage, SDS-PAGE, ChIP/RT-qPCR, Agarose Gel Electrophoresis, etc.
Software:
Prism-8, Image-J, Photoshop CS6, Microsoft Office, Biorender, EndNote, etc.
In Vivo:
Xenograft experiment using BALB/c mice, FATAX assay using fertilized embryos of Xenopus laevis, etc.
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