Laboratory Skills

• Animal Handling (Mice): Blood Withdrawal (Retro-orbital, IV), Injections (IP, IV), Dosage (Catheter), Dissection, Tissue Processing (Bone marrow, Spleen, Lymph nodes, Small Intestine and Large Intestine)

• Bacterial and Animal Cell Culture

• Microscopy (Light, Fluorescence, TEM and SEM)

• Flow cytometry and Fluorescence Activated Cell Sorter (FACS)

• DNA/Plasmid cloning, Site-Directed Mutagenesis, PCR, Bacterial Transformation, DNA/RNA Extraction and Purification

• Agarose Gel Electrophoresis, SDS-PAGE, Western Blotting, ELISA

• Protein Expression and Purification

• Chromatography (FPLC and Affinity techniques)

• UV/Vis spectrophotometry, Fluorescence spectroscopy

• Nanoparticle synthesis

• In vitro assays: Immunofluorescence assays, osteoclastogenesis/osteoblastogenesis assays, MTT cytotoxicity assay.