Education & expertise

Laboratory & technical expertise

Molecular biology

DNA and plasmid cloning; site-directed mutagenesis; PCR; bacterial transformation; DNA/RNA extraction and purification; agarose gel electrophoresis.

Protein biochemistry & analytical methods

Protein expression and purification; FPLC and affinity chromatography; SDS-PAGE; Western blotting; ELISA; UV/visible spectrophotometry; fluorescence spectroscopy.

Cell biology & imaging

Bacterial and animal cell culture; light and fluorescence microscopy; transmission and scanning electron microscopy (TEM/SEM); flow cytometry and fluorescence-activated cell sorting (FACS).

Experimental models & tissue processing

Mouse handling; retro-orbital and intravenous blood withdrawal; intraperitoneal and intravenous injections; catheter-based dosing; dissection; processing of bone marrow, spleen, lymph nodes, small intestine and large intestine.

Functional assays & materials

Immunofluorescence assays; osteoclastogenesis and osteoblastogenesis assays; MTT cytotoxicity assays; nanoparticle synthesis.