Date: Sunday 23rd May,2021

Monday 24th May, 2021

Time: 08:00 P.M.

Link to attend:

Day 1 [Press to attend]

Day 2 [Press to attend]

About the workshop:

Viral isolation is essential for confirming diagnosis of newly emerged viral infections, preparation of vaccines and performing different  researches on viruses. Tissue culture is  a common method for viral isolation, in addition to other methods such as, animal and embryonated egg inoculation  . It needs a specially equipped  lab, experienced well trained personells, and strict measures for infection control to avoid culture  contamination, laboratory acquired  infection or spreading of viruses outside the lab to surroundings environment. The lab should be provided with at least level 2 safety laminar flow, Inverted microscope, CO2 incubator, water bath and hemocytometer. Vessels with different shapes could be used together with a suitable growth media to mention the growth and viability of cells. Different types of cells could be used according to expected virus. Primary tissue culture could be prepared from organs of human and animal or provided commercially. Subculture  is needed to allow cell dilution, growth and distribution in multiple vessels, a procedure that needs trypsin/EDTA mixture to dissociate cells from each others and from used vessels. Different methods could be used to detect viral growth and count. Many factors can affect the  success of viral isolation in tissue culture  such as sample processing and transfer, nature of the virus and suitability of the used cells.