Hyp-ACB
Description
Understanding how metabolism and gene expression can be turned on or off in a concerted manner allows unravelling the molecular mechanisms underlying physiological metabolic homeostasis and what goes awry in disease. It will also provide information on how biological systems react to environmental stimuli. Possible applications of this technology include multiple areas of biological research, spanning from molecular oncology, cardiovascular diseases, immunology, neurodegeneration, metabolic disorders, ageing, stem cells,tissue engineering, host-pathogen interactions, toxicology, drug discovery, environmental biology, bioengineering, nutrition etc.
The Hyp-ACB facility is a complex infrastructure (active from end 2018) able to measure, under normoxic and hypoxic conditions, the metabolic activity of living cells, with particular attention to the respiratory activity of mitochondria, in parallel with analysis of gene expression. The facility allows monitoring cell metabolism and gene expression under variable oxygen tensions, thus reproducing more closely the cell or tissue microenvironment(s) in both physiological and pathological conditions.
A more detailed description of the facility can be found HERE
The Hyp-ACB laboratory includes the following equipment :
Seahorse XFe96 Agilent
Hypoxic cabin Don Whitley mod. i2 Workstation
HypoxyLab™ Bench-top physiological oxygen incubator and workstation
Sterile hood Thermo Scientific MSC ADVANTAGE 1.2
Incubator Thermo Scientific FORMA Steri-cycle 250i
Microscope EVOS XL CORE Invitrogen
Real-Time PCR Applied Biosystems QuantStudio 3
Basic support equipment for molecular/cell biology is also available.
STAFF
Francesca Cutruzzolà - Managing Director - Full professor - E-mail francesca.cutruzzola@uniroma1.it SCOPUS
Serena Rinaldo- Data Analysis Manager - Associate Professor - E-mail serena.rinaldo@uniroma1.it SCOPUS
Alessio Paone- Cell biology - Researcher - E-mail alessio.paone@uniroma1.it SCOPUS
Giovanna Boumis - Technician - E-mail giovanna.boumis@uniroma1.it SCOPUS
How to access the Facility
Access to the Facility can be requested by filling the APPLICATION FORM
Before applying, please carefully read the USER GUIDELINES
The platform is located in the Sapienza Main Campus, Building CU027, Rooms S32/S33 (Floor -1).
The Infrastructure is a member of:
