ARP
Description
The ARP facility allows to measure rapid kinetic processes (micro- to milliseconds) on purified macromolecules in vitro by different spectroscopies (absorbance, fluorescence).
An array of instruments are available, including stopped-flow instruments (msec-time frame ≥ 10 ms) to study folding/unfolding processes, enzyme reaction mechanisms, rapid binding or dissociation processes (absorbance and fluorescence). For faster measurements, a Hi-Tech PTJ-64 capacitor-discharge T-jump apparatus ( ≥ 20 μs) for fluorescence and absorbance experiments is available, as well as an in-house built continuous flow (original prototype) based on a capillary mixing methodology (dead-time 50 μs).
The ARP laboratory includes in detail the following equipment:
Applied Photophysics single-mixing -SX-18
Applied Photophysics Pi-star 180,
Applied Photophysics sequential-mixing DX-17MV
Tgk Scientific Hi-Tech PTJ-64 capacitor-discharge T-jump apparatus
In-house built continuous flow (original prototype) based on a capillary mixing methodology (dead-time 50 μs) with a fluorescence CCD camera
Spectrofotometers/fluorimeters for general analysis of samples before kinetic measurements are also available.
STAFF
Francesca Cutruzzolà - Managing Director - Full professor - E-mail francesca.cutruzzola@uniroma1.it SCOPUS
Stefano Gianni - Scientific Officer- Full Professor - E-mail stefano.gianni@uniroma1.it SCOPUS
Andrea Bellelli - Full Professor - E-mail andrea.bellelli@uniroma1.it SCOPUS
Roberto Contestabile- Associate Professor - E-mail roberto.contestabile@uniroma1.it SCOPUS
Francesco Malatesta - Full Professor - E-mail francesco.malatesta@uniroma1.it SCOPUS
Angelo Toto- Researcher- E-mail angelo.toto@uniroma1.it. SCOPUS
How to access the Facility
Access to the Facility can be requested by filling the APPLICATION FORM
Before applying, please carefully read the USER GUIDELINES
The facility is located in the Sapienza Main Campus, Building CU027, Room T5 (Ground Floor).