2025 Research Projects
2025 Research Projects
Home Institution: South Carolina State University
Mentors: Mike Patrick, Kate Fessler,
and Doug Vines
Faculty Mentor: Dr. Matt Clark
The genus Rubus, part of the rose family (Rosaceae), includes economically and ecologically important plants such as blackberries, raspberries, and dewberries. Despite their familiarity, many Rubus species are extremely difficult to distinguish due to frequent hybridization, apomixis (asexual seed production), and overlapping morphological traits. This research examines three species in the rose family from the Rubus genus: Rubus alumnus, Rubus allegheniensis, and Rubus rosa. Through a combination of field observations, specimen analysis, and comparative classification with the help of measuring software, this study focuses on contrasting the leaf and inflorescence structures of primocanes (first year growth shoots) and floricanes (second year growth shoots which bear fruit). The aim is to distinguish key traits that define species boundaries and improve differentiation among these closely related taxa. These findings will help clarify distinguishing characteristics, support species identification, and guide further research into species variation within Rubus.
Cover crops are known for their contributions to crop production, with ongoing research investigating their influence on soil-borne pathogens. One such fungal pathogen, Rhizoctonia solani, causes seedling blight across a range of Minnesota crops. Management of this pathogen is complicated due to its persistence in soil as sclerotia. However, cover crops may help suppress soil-borne pathogens by altering soil health. This study investigated how cover cropping influences the viability of Rhizoctonia solani sclerotia. Field trials were established in Crookston and St. Paul, Minnesota in 2023, and planted to brown mustard and winter rye in fall of both 2023 and 2024. Sclerotia were incubated beneath each cover crop at depths of both 10 cm and 30 cm. Mesh bags containing sclerotia were buried for one season following a previous year of cover cropping, while others remained in the soil for both years. All bags were retrieved at planting in spring 2025. After two years in Crookston, sclerotia viability was higher at 30 cm than 10 cm (p = 0.02), with no impact from cover crops. This research contributes to a better understanding of how cover crops influence pathogen persistence, with broader implications for plant and soil health management.
Home Institution: Florida International University
Mentor: Hunter Kluegel
Faculty Mentor: Dr. Megan McCaghey
Home Institution: Baldwin Wallace University
Mentor: Jillian Turbeville
Faculty Mentor: Dr. Dominic Petrella
Kentucky Bluegrass (Poa pratensis) is a cool-season turfgrass used widely on athletic fields, golf courses, and homelawns. A drawback to using Kentucky bluegrass is its slow and sporadic germination rate. Germination stimulants are frequently used to improve germination rate, but little is known on their effects on Kentucky bluegrass. One such stimulant is gibberellins, a class of plant hormones found endogenously in seeds. Smoke has also been found to be a germination stimulant as it is abundant in karrikin hormones. Kentucky bluegrass (‘Tirem’) seeds were imbibed in varying concentrations of liquid smoke, pure karrikin (KAR1), gibberellin A1 (GA1), GA3, GA4, or GA7. Seeds were also imbibed in water (negative control) 0.20% KNO3 (positive control). Following imbibition, seeds were placed on germination blotter paper in honeycomb boxes within a growth chamber (25°C day, 20°C night, 12 hr. Photoperiod, 100 µmol m-2 s-1) to monitor germination rate. As the concentration of liquid smoke increased, the germination rate decreased. KAR1 showed a similar effect to GA4 and KNO3 showed the largest germination rate. All gibberellins produced an increase in germination except for GA1. Future experiments will examine the interaction between KNO3 and GA4/7 due to their positive, independent, effects on germination.
High tunnels (HTs) are increasingly used by Minnesota farmers to increase crop productivity, protect against extreme weather, and extend growing seasons. These structures create a unique microclimate which varies in temperature, humidity, and soil moisture compared to open-field environments. To monitor these conditions, we used temperature data loggers inside and outside the HTs to track soil and air temperature fluctuations. Limited rainfall in HTs can lead to nutrient buildup from manure fertilizer use. A potential alternative to manure fertilizer is legume cover crops (CC), which fix nitrogen and enhance soil fertility, allowing for reduced fertilizer use. To compare legume CC on N availability, we collected soil from Grand Rapids, Minnesota HT with two legume CC: hairy vetch (Vicia villosa) and austrian winter pea (Pisum sativum subsp. arvense), then measured Potentially Mineralized Nitrogen to assess soil quality. To further explore nitrogen fixation potential, we evaluated the effects of rhizobia inoculants on two additional legume CCs: crimson clover (Trifolium incarnatum) and field pea (Pisum sativum) in an open field by measuring nodulation and plant biomass. These experiments contribute to understanding of how legume CC can be integrated into HT systems to improve soil fertility and sustainable nutrient management.
Home Institution: California State University Monterey Bay
Mentors: Dr. Thanwalee Sooksa-nguan (JiJY), Ben Tanner
Faculty Mentor: Dr. Julie Grossman
Home Institution: Concordia University
Mentor: Timileyin Summonu
Faculty Mentor: Dr. Laura Shannon
The cultivated potato (Solanum tuberosum) is typically bred as a tetraploid clonal crop and propagated asexually. However, tetraploid breeding often results in low genetic gain due to high genetic load and heterozygosity. Diploid breeding offers a simpler genetic structure, higher genetic gain, and a faster response to emerging challenges in potato production. In this study, we compared two populations derived from the Red Norland potato variety: 200 selfed tetraploid progeny and 200 dihaploid progeny generated using the IVP101 dihaploid inducer. All plants were grown in a greenhouse for tuber multiplication prior to field evaluation. In total, 400 genotypes, 187 of which were replicated, were established in the field to assess phenotypic differences associated with changes in ploidy level. We are collecting data on key morphological and agronomic traits, including date of row closure, flowering time, flower color, leaf color, and chlorophyll content. By analyzing these traits, we aim to better understand how reducing ploidy from tetraploid to diploid affects plant development and phenotype. These findings will help inform breeding decisions, facilitate the use of diploid lines in potato improvement programs, and contribute to broader efforts to develop more efficient and genetically tractable potato cultivars.
Northern Wild Rice (Zizania palustris) is a culturally significant aquatic crop native to North America. A major obstacle to its cultivation is seed shattering, where mature seeds prematurely detach from the plant, causing substantial yield losses during harvest. This project aims to identify allelic variants of genes potentially influencing seed shattering. Using molecular biology techniques DNA extraction, PCR amplification, and Sanger sequencing we analyzed five wild rice populations: NEBr, Franklin, Netum, K2, and Dawn-SR. To evaluate each population, 32 plants were grown under greenhouse conditions, arranged in a 4 by 4 planting design. We focused on two candidate genes that were selected based on comparative genomics, gene expression profiles, and prior genome-wide association. By contrasting individuals exhibiting strong seed retention with those that shatter early, we seek to discover genetic markers, such as single nucleotide polymorphisms (SNPs), that may associated with seed shattering. These insights will facilitate targeted breeding approaches to enhance harvest efficiency and promote sustainable production of Northern Wild Rice.
Home Institution: Virginia State University
Mentors: Kat Schmidt, Lillian McGlip
Faculty Mentor: Dr. Jennifer Kimball
Home Institution: South Carolina State University
Mentors: Dr. Seth Wannemuehler
and Herika Pessoa
Faculty Mentor: Dr. Brandon Miller
American hazelnut (Corylus americana Marshall) is gaining attention as a climate-resilient, regionally adapted nut crop with growing economic potential in the Upper Midwest. Hazelnut processing generates husks as a byproduct, which are typically discarded despite their potential value as mulch. This study evaluated the effectiveness of American hazelnut husks as a sustainable mulch alternative in container plant production. A greenhouse experiment using a randomized complete block design with seven blocks compared five mulch treatments: control (no mulch), American hazelnut husks, rice hulls, bark, and sawdust. Each block included one replicate of each treatment, one pot filled with a commercial substrate, planted with petunia, and inoculated with ryegrass (Lolium spp.) and white clover (Trifolium repens). Over 30 days, we recorded substrate moisture, substrate temperature, surface temperature, each weed emergence, and plant growth. Data were analyzed using ANOVA and Tukey’s HSD for mean separation (α = 0.05). Compared to the commonly used mulch materials, hazelnut husks were as effective, or superior, in suppressing weed emergence while maintaining moisture and temperature and supporting plant growth comparable to conventional materials. Our findings highlight the possibility of repurposing American hazelnut husks as an effective, locally sourced mulch material for use in container production.
Erosion of nutrient-rich topsoil across cropping systems poses a problem for soil health and water quality. Measuring runoff, however, is challenging due to the cost and complexity of field monitoring equipment. In this study, we tested a high-throughput method using erosion mats to assess soil and nutrient loss under different cropping systems: perennial, cover crop, and conventional. The mats, 6 x 6” fabric squares pinned to the soil, were deployed before and during the early planting season to align with peak erosion risk from spring rainfall. Mats were deployed at three sites (one at Farm at the Arb, MN, and two in Redwood Falls, MN) for the 2024 and 2025 planting seasons. In Year 2, an ion-exchange resin packet was added to the Redwood Falls sites' mats to capture nitrate (NO3-) from surface runoff. While no significant difference was found between sites, concentrations did differ significantly between deployments. Soil movement was quantified by the mass of soil collected from mats after deployment. Movement was greater in conventional systems, but there was no significant difference in the collected soil's C:N ratios across cropping systems. Sediment from mats was slightly correlated with total precipitation during each deployment across years.
Home Institution: Hamline University
Mentors: Kat LaBine and Leah Hallet
Faculty Mentor: Dr. Anna Cates and Dr. Jessica Gutknecht
Home Institution: Metro State University
Mentors: Dr. Erin Treiber and Madan Pandey
Faculty Mentors: Dr. Soon Li Teh
Efficiently identifying prominent grapevine phenotypic traits such as canopy, fruit color, and fruit size is a critical part of grape breeding. Phenotyping is both time-intensive and relies on direct observations to determine the severity of disease symptoms. The phenotyping process can be expedited by using high-resolution images that can be manually annotated for valuable traits and subsequently trained on a convolutional neural network (CNN) to enable future prediction of disease symptoms/scores based on the acquired images. This would allow quicker, efficient, and objective phenotyping. At the University of Minnesota’s Horticulture Research Center, this technology is being used to phenotype diseases and other breeding traits, including berry color and cluster size. A utility vehicle fitted with a stereo camera was used to photograph a family of 955 grapevines. Select photographs were evaluated using image-based plant phenotyping to assess and annotate the breeding traits. In the future, the annotated images and field observations will be used to train a CNN model that can identify common diseases and other valuable traits with less time and resources for accelerating breeding programs.
Drosophila suzukii, or spotted-wing drosophila (SWD), is an invasive fly distinguished by its serrated ovipositor used by females to lay eggs directly into ripe or ripening fruit. SWD prefers berries and stone fruit but can also survive on nearby non-crop plants such as Lonicera spp. (honeysuckle). This alternative host provides resources for the pest when fruit crop hosts are not present. Lonicera spp. plants fruit sooner than many other hosts, making them favorable. To manage this pest, researchers explored its native habitat in Southeast Asia and identified several parasitoid wasps. One of them, G. kimorum, is a specialist that attacks SWD and was recently approved for biological control releases. Simultaneously, a generalist parasitoid, L. japonica, was detected in the U.S. This study aims to identify and investigate parasitoid populations in Lonicera spp. fruit across rural and urban areas to determine the relative efficacy of parasitism for biological control to suppress SWD populations.We conducted this research by collecting samples weekly from six sites,three urban campus sites and three rural. At each site, we collected thirty fruits from a single branch. Samples were incubated to allow parasitoid emergence, and PCR analysis was used to accurately identify species.
Home Institution: St. Olaf College
Mentor: Jay Delacy
Faculty Mentor: Dr. Mary Rogers