Ganesh Ramaprasad

Data Blitz Presentation

"Identifying the role of different domains in yeast methyltransferase Set5"

SET domain is a well characterized histone lysine methyltransferase(KMTase). We are attempting to identify which regions of Set5 protein, besides the SET domain, are involved in KMTase activity.

Poster Presentation

Characterizing the role of MYND domain and C-terminus of Set5 in S. cerevisiae

Histones are proteins around which DNA is wrapped to be condensed in the nucleus of the cell. Besides functioning as structural proteins, they also have an important role in regulating the expression of genes. Set5, a histone lysine methyltransferase in Saccharomyces cerevisiae, adds mono methyl marks to lysine residues on Histone 4 at residues 5,8 and 12. The Set5 protein consists of multiple domains and this includes the SET domain, MYND domain, post-SET domain and C-terminus. In order to answer which domains of Set5 are involved in methylation activity, we made different mutants of SET5 using Gibson assembly and these included Set5ΔMYND, Set5ΔC-terminus and Set5 with C-terminus domain deleted after the post-SET domain. Our result shows that deletion of the MYND domain has no effect on the methylation activity of Set 5 but deletion of the C-terminus abolished the methylation activity. Diminished methylation activity was only restored when an excess of Set5ΔC-terminus was used.