WP1: INTRAGENERATIONAL NUTRITIONAL PROGRAMMING EFFECTS

Aim: To determine the influence of early nutritional intervention on the phenotype and the epigenome.

This WP will contribute to:

· Elucidate the cause-effect relationship of carbohydrate content, dietary fatty acid (FA) profile on the epigenetic and phenotypic outcomes

· Assess the reversibility of the epigenetic marks induced by the nutritional programming and the resulting phenotypes

· Generate a better mechanistic understanding of nutritional programming

Research questions:

· How dietary carbohydrate content, FA profile and the interplay between them translate into epigenetic signatures?

· What are the specific roles of DNA methylation, histone modifications and lncRNA induced by carbohydrate content and dietary fatty acid profile early intervention on gene expression and metabolism long-term?

· How do those changes in gene expression translate into physiologically relevant phenotypes?