There is a need for a standardized protocol to create biomimetic muscle tissue constructs in vitro for research and therapeutic purposes. A hydrogel scaffold was created through a process of decellularization of chopped porcine tissue in 1% SDS for 3 days, 20X IPA for 24 hours, then milling, lyophilization, and digestion in pepsin and hydrochloric acid. Histology and DNA quantification testing was performed to confirm the number of wash days for decellularization, and tissues were found to be thoroughly decelled with the protocol. Future directions for the project include successfully forming hydrogels in designed biocompatible molds, using laser-cutting for methodical shaping of the tissue, and finally seeding stem cells into the scaffolds to create functional biomimetic tissue in vitro