There is a need for accurate 3D imaging of cartilage in order to construct bioengineering scaffolds for patients suffering from osteoarthritis. The current digital volumetric imaging methodology produces detailed 3D reconstruction for tissues, but has issues with the tissue sample migrating, the inadvertent release of black cut material, inconsistent brightness and exposure levels, and a lack of a fluorescent standard. To alleviate the first major problem, a mold was designed in Solidworks to aid in identifying how to place foam to reduce tissue migration to hold the tissue in place while setting in resin. The second problem was addressed by repositioning the blowing and vacuum hoses to properly suction black cut material, which was deemed successful via slow-motion video. The last two problems were solved by creating a fluorescent hydrogen with Dragon Green beads to serve as a fluorescent standard when imaging different tissues.