Extracellular matrix (ECM) biomaterials are an exciting tool for tissue engineering as these materials are derived from the protein scaffolding naturally found in organs such as the heart and possess both mechanical and biochemical properties that are known to influence cellular behavior. Decellularization, a crucial step in obtaining ECM, involves using detergents with varying effects on the ECM’s chemical structure as it can strip away vital biochemical signals within ECM such as glycosaminoglycans, proteoglycans, and certain basement membrane proteins. While sodium dodecyl sulfate (SDS) is commonly used due to its high efficiency, it may strip away more of these components, prompting the exploration of milder alternatives like sodium deoxycholate (SDC) to better preserve these biochemical signals essential for tissue engineering and therapeutic application. Additionally, we have developed grips and molds to generate EHT’s from our ECM products and we plan on testing passive and active mechanics for each chamber and detergent method before seeding hydrogels with human induced pluripotent stem cells.