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Islet cell transplantation is a novel treatment being explored for diabetes. To better understand the effectiveness of this treatment, we are investigating the extent of innervation of islet cell transplants through multiple tissue imaging methods.Â
Islet cell transplantation is a novel treatment being explored for diabetes. To better understand the effectiveness of this treatment, we are investigating the extent of innervation of islet cell transplants through multiple tissue imaging methods.Â
- Traditional Immunohistochemistry
a. Tissue from transplant is removed at varying timepoints after the transplantation
b. The transplant tissue is then placed on slides and immunohistochemistry is performed to be able to visualize axons
c. Using a fluorescence microscope, we are able to see whether the transplants have been successfully innervated
2. Tissue Clearing and Immunohistochemistry
2. Tissue Clearing and Immunohistochemistry
a. Tissue from transplant is removed at varying timepoints after the transplantation
b. The transplant tissue is then processed to remove all colour from the cells, making the tissue translucent
c. Immunohistochemistry is performed on whole tissue samples
d. Using a fluorescence microscope, we are able to see whether the transplants have been successfully innervated in 3D
This method allows us to better visualize the path of axonal growth.
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