PUBLICATIONS　研究業績

2025

• Xuhao Huang, Hirotaka Kanoh, Jumpei Ueno, Ushio Ishikawa, Takahiro Nitta,  Masayuki Takamatsu, Atsushi Shimoyama, Makoto Taniguchi, Shinya Hanashima, Kei-ichiro Inamori, Jin-ichi Inokuchi, Koichi Fukase, Kazuya Kabayama,  Modulation function of sphingomyelin molecular species in TLR4 signaling and cell death. Cell Reports, 2025, 44, 116568. https://doi.org/10.1016/j.celrep.2025.116568

• Shinya Hanashima;* Takafumi Asahina; Raymond Malabed; Katsuaki Sasaki; Michio Murata, Effect of glucosylation for the vertical movement of cholesterol in bilayer membranes BBA - Biomembranes, 2025 1867(5–6), 184422. https://doi.org/10.1016/j.bbamem.2025.184422

• Tomokazu Yasuda,* Daiki Ueura, Madoka Nakagomi, Shinya Hanashima, Michio Murata, Relevance of ceramide 1-phosphate domain formation in activation of cytosolic phospholipase A2, Biophysical Chemistry, 2025, 322, 107433, https://doi.org/10.1016/j.bpc.2025.107433.

• Kenichi G. N. Suzuki,* Koichiro M. Hirosawa, Tatsuki Isogai, Tomokazu Yasuda, Shinya Hanashima*  Membrane Dynamics of Exosomes as Revealed by Single-Molecule Imaging, In: Baba, Y., Hanayama, R., Akita, H., Yasui, T. (eds) Extracellular Fine Particles. Springer, Singapore. 2025, 69–80. https://doi.org/10.1007/978-981-97-7067-0_6 

2024

• Shinya Hanashima,* Ayana Yamanaka, Yuki Ibata, Tomokazu Yasuda, Yuichi Umegawa,, Michio Murata Lipid compositions of liquid-ordered and liquid-disordered phases in ternary membranes of sphingomyelin, cholesterol, and dioleoylphosphatidylcholine determined by 2H NMR: stearoyl-sphingomyelin compared with its palmitoyl counterpart. Langmuir 2024, 40, 22973-22981.

https://doi.org/10.1021/acs.langmuir.4c03104

• Darcy Garza-Miyazato, Shinya Hanashima,* Yuichi Umegawa, Michio Murata,* Masanao Kinoshita, Nobuaki Matsumori, Peter Greimel  Mode of molecular interaction of triterpenoid saponin ginsenoside Rh2 with membrane lipids in liquid-disordered phases BBA Biomembranes 2024, 1866(7), 184366. https://doi.org/10.1016/j.bbamem.2024.184366

• Tomokazu Yasuda, Daiki Ueura, Madoka Nakagomi, Shinya Hanashima, J. Peter Slotte, Michio Murata　Design, synthesis of ceramide 1-phosphate analogs and their affinity for cytosolic phospholipase A2 as evidenced by surface plasmon resonance. Bioorganic & Medicinal Chemistry Letters 2024, 107, 129792. https://doi.org/10.1016/j.bmcl.2024.129792 

2023

• Hirofumi Watanabe, Shinya Hanashima*, Yo Yano, Tomokazu Yasuda, Michio Murata* Passive Translocation of Phospholipids in Asymmetric Model Membranes: Solid-State 1H NMR Characterization of Flip–Flop Kinetics Using Deuterated Sphingomyelin and Phosphatidylcholine Langmuir 2023, 39, 15153-15408. https://doi.org/10.1021/acs.langmuir.3c01650

• Shinya Hanashima,* Mikito Nakano, Michio Murata Dynamic assembly and interaction of glycosphingolipids in cholesterol-containing model membranes Pure and Applied Chemistry 2023  95, 971-977. https://doi.org/10.1515/pac-2023-0204

• Joan Candice Ondevilla , Shinya Hanashima,* Akane Mukogawa, Darcy Garza Miyazato, Yuichi Umegawa, Michio Murata* Effect of the number of sugar units on the interaction between diosgenyl saponin and membrane lipids BBA-Biomembranes 2023, 1865, 184145. https://doi.org/10.1016/j.bbamem.2023.184145 

• Tomokazu Yasuda, J. Peter Slotte, Michio Murata, Shinya Hanashima* Molecular Dynamics of Glycolipids in Liposomes, Methods in molecular biology (Clifton, N.J.) 2023, 2613, 257-270. https://doi.org/10.1007/978-1-0716-2910-9_19  

• Tomokazu Yasuda*, Hirofumi Watanabe, Koichiro M. Hirosawa,  Kenichi G. N. Suzuki, Keishi Suga, Shinya Hanashima* Fluorescence Spectroscopic Analysis of Lateral and Transbilayer Fluidity of Exosome Membranes Langmuir 2022, 38, 48, 14695–14703. https://doi.org/10.1021/acs.langmuir.2c02258 

2022年以前 (by Shinya Hanashima)

PAPERS

1. 1. Shinya Hanashima,* Kanako Mito, Yuichi Umegawa, Michio Murata, Hironobu Hojo Lipid chain-driven interaction of a lipidated Src-family kinase Lyn with the bilayer membrane. Org. Biomol. Chem. 2022, 20, 6436-6444.

   2. Larcy Garza, Shinya Hanashima,* Yuichi Umegawa, Michio Murata, Masanao Kinoshita, Nobuaki  Matsumori, Greimel Peter, The behavior of triterpenoid saponin ginsenoside Rh2 in ordered and disordered phases in model membranes consisting of sphingomyelin, phosphatidylcholine, and cholesterol. Langmuir 2022, 38, 10478–10491.

   3. Yuichi Umegawa, Tomoya Yamamoto, Mayank Dixit, Kosuke Funahashi, Sangjae Seo, Yasuo Nakagawa, Taiga Suzuki, Shigeru Matsuoka, Hiroshi Tsuchikawa, Shinya Hanashima, Tohru Oishi, Nobuaki Matsumori, Wataru Shinoda, Michio Murata. Amphotericin B assembles into seven-molecule ion channels: An NMR and molecular dynamics study.　SCIENCE ADVANCES 2022, 8, eabo2658.

   4. Shinya Hanashima,* Ryuji Ikeda, Yuki Matsubara, Tomokazu Yasuda, Hiroshi Tsuchikawa, J Peter Slotte, Michio Murata. Effect of cholesterol on the lactosylceramide domains in phospholipid bilayers. Biophys. J. 2022, 121, 1143-1155.

   5. Shinya Hanashima, Takanori Nakane, Eiichi Mizohata*, Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins. Membranes 2021, 11, 823.

   6. Mikito Nakano, Shinya Hanashima,* Toshiaki Hara, Kazuya Kabayama, Yuya Asahina, Hironobu Hojo, Naoko Komura, Hiromune Ando, Thomas K. M. Nyholm, J. Peter Slotte, Michio Murata*. FRET Detects Lateral Interaction between Transmembrane Domain of EGF Receptor and Ganglioside GM3 in Lipid Bilayers. Biochim. Biophys. Acta. Biomembranes 2021, 1863, 183623.

   7. Joan Candice Ondevilla, Shinya Hanashima*, Akane Mukogawa, Yuichi Umegawa, Michio Murata*, Diosgenin-induced physicochemical effects on phospholipid bilayers in comparison with cholesterol. Bioorg. Med. Chem. Lett. 2021, 36, 127816.

   8. Hitoshi Ishiwata,* Hiroshi C. Watanabe, Shinya Hanashima, Takayuki Iwasaki, Mutsuko Hatano. Label‐Free Phase Change Detection of Lipid Bilayers Using Nanoscale Diamond Magnetometry, Advanced Quantum Technologies, 2021, 2000106.

   9. Shinya Hanashima,* Nanami Fukuda, Raymond Malabed, Michio Murata,* Masanao Kinoshita, Peter Greimel, Yoshio Hirabayashi. β-Glucosylation of cholesterol reduces sterol-sphingomyelin interactions.  Biochim. Biophys. Acta. Biomembranes, 2020, 183496.

   10. Yo Yano, Shinya Hanashima, Hiroshi Tsuchikawa, Tomokazu Yasuda, J Peter Slotte, Erwin London, Michio Murata. Sphingomyelins and ent-Sphingomyelins Form Homophilic Nano-Subdomains within Liquid Ordered Domains. Biophys. J. 2020, 119, 539-552.

   11. Nao Yamakawa, Yu Yasuda, Atsushi Yoshimura, Ami Goshima, Paul R. Crocker, Gérard Vergoten, Yuji Nishiura, Takashi Takahashi, Shinya Hanashima, Kana Matsumoto, Yoshiki Yamaguchi, Hiroshi Tanaka, Ken Kitajima, Chihiro Sato. Discovery of a New Sialic Acid Binding Region That Regulates Siglec-7. Scientific Report 2020 10, 8647.

   12. Maina Takahashi, Junya Shirasaki, Naoko Komura*, Katsuaki Sasaki, Hide-Nori Tanaka, Akihiro Imamura, Hideharu Ishida, Shinya Hanashima,* Michio Murata, Hiromune Ando*. Efficient Diversification of GM3 Ganglioside via Late-Stage Sialylation and Dynamic Glycan Structural Studies with 19F Solid-State NMR. Org. Biomol. Chem. 2020, 18, 2902-2913.

   13. Hiroki Kusaka, Shunsuke Kita, Takashi Tadokoro, Kouki Yoshida, Yoshiyuki Kasai, Harumi Niiyama, Yukari Fujimoto, Shinya Hanashima, Michio Murata, Shigeru Sugiyama, Toyoyuki Oseae, Kimiko Kuroki, Katsumi Maenaka. Efficient Preparation of Human and Mouse CD1d Proteins Using Silkworm Baculovirus Expression System. Protein Expression and Purification 2020, 22, 105631.

   14. Raymond Malabed, Shinya Hanashima,* Michio Murata,* Kaori Sakurai. Interactions of OSW-1 with Lipid Bilayers in Comparison with Digitonin and Soyasaponin. Langmuir 2020, 36, 3600-3610.

   15. Tomoya Yamamoto, Yuichi Umegawa, Hiroshi Tsuchikawa, Shinya Hanashima, Nobuaki Matsumori, Kosuke Funahashi, Sangjae Seo, Wataru Shinoda, Michio Murata. Amphotericin B-Ergosterol Complex Spans a Lipid Bilayer as a Single-Length Assembly. Biochemistry 2019, 58, 5188-5196.

   16.  Yoshiyuki Manabe, Roberta Marchetti, Yohei Takakura, Masahiro Nagasaki, Wataru Nihei, Tomoyuki Takebe, Katsunori Tanaka, Kazuya Kabayama, Fabrizio Chiodo, Shinya Hanashima, Yoshihiro Kamada, Eiji Miyoshi, Hari Prasad Dulal, Yoshiki Yamaguchi, Yoshiyuki Adachi, Naohito Ohno, Hiroshi Tanaka, Alba Silipo, Koichi Fukase, Antonio Molinaro. The Core Fucose on an IgG Antibody is an Endogenous Ligand of Dectin-1. Angew. Chem. Int. Ed. Engl. 2019, 58,18697-18702.

   17. Shinya Hanashima,* Yuki Ibata, Hirofumi Watanabe, Tomokazu Yasuda, Hiroshi Tsuchikawa, Michio Murata*. Side-Chain Deuterated Cholesterol as a Molecular Probe to Determine Membrane Order and Cholesterol Partitioning. Org. Biomol. Chem. 2019, 17, 8601-8610.

   18. Shinya Hanashima,* Kazuhiro Murakami, Michihiro Yura, Yo Yano, Yuichi Umegawa, Hiroshi Tsuchikawa, Nobuaki Matsumori, Sangjae Seo, Wataru Shinoda, Michio Murata*. Cholesterol-Induced Conformational Change in the Sphingomyelin Headgroup. Biophys. J. 2019, 117, 307-318.

   19. Tomoya Yamamoto, Yuichi Umegawa, Masaki Yamagami, Taiga Suzuki, Hiroshi Tsuchikawa, Shinya Hanashima, Nobuaki Matsumori, Michio Murata*. The Perpendicular Orientation of Amphotericin B Methyl Ester in Hydrated Lipid Bilayers Supports the Barrel-Stave Model. Biochemistry 2019, 58, 2282-2291.

   20. Fumi Yoshida, Hiroki Yoshinaka, Hidenori Tanaka, Shinya Hanashima, Yoshiki Yamaguchi, Mikio Ishihara, Miyuki Saburomaru, Yuki Kato, Risa Saito, Hiromune Ando, Makoto Kiso, Akihiro Imamura,* Hideharu Ishida*. Synthesis of Core Oligosaccharides of Lipooligosaccharides from Campylobacter jejuni, a Putative Cause of Guillain–Barré Syndrome. Chem. Eur. J. 2019, 25, 796-805.

   21. Yo Yano, Shinya Hanashima, Tomokazu Yasuda, Hiroshi Tsuchikawa, Nobuaki Matsumori, Masanao Kinoshita, Md. Abdullah Al Sazzad, J. Peter Slotte*, Michio Murata*. Sphingomyelin Stereoisomers Reveal That Homophilic Interactions Cause Nanodomain Formation. Biophys J. 2018, 115, 1530-1540.

   22. Kimberly Cornelio, Rafael A Espiritu, Shinya Hanashima, Yasuto Todokoro, Raymond Malabed, Michio Murata*, Masanao Kinoshita, Nobuaki Matsumori, Shinichi Nishimura, Hideaki Kakeya, Minoru Yoshida, Shigeki Matsunaga. Theonellamide A, a Marine-Sponge-Derived Bicyclic Peptide, Binds to Sterol in Aqueous DMSO: Solution NMR-Based Analysis of Peptide-Sterol Interactions Using Hydroxylated Cholesterol. Biochim. Biophys. Acta. Biomembranes 2018, 1861, 228-235.

   23. Masamichi Nagae*, Yasuhiko Kizuka, Emiko Mihara, Yu Kitago, Shinya Hanashima, Yukishige Ito, Junichi Takagi, Naoyuki Taniguchi, Yoshiki Yamaguchi Structure and Mechanism of Cancer-Associated Glycosyltransferase, N-Acetylglucosaminyltransferase-V. Nat. Commun. 2018, 9, 3380.

   24. Yumiko Ohhashi, Yoshiki Yamaguchi, Hiroshi Kurahashia, Yuji O. Kamatari, Shinju Sugiyama, Boran Uluca Timo Piechatzek, Yusuke Komi, Toshinobu Shida, Henrik Müller, Shinya Hanashima, Henrike Heise,Kazuo Kuwata, Motomasa Tanaka*. Molecular Basis for Diversification of Yeast Prion Strain Conformation. Proc. Natl Acad. Sci. 2018, 115, 2389-2394.

   25. Shinya Hanashima, Akitsugu Suga, Yoshiki Yamaguchi*. Bisecting GlcNAc Restricts Conformations of Branches in Model N-Glycans with GlcNAc Termini. Carbohydr. Res. 2018, 456, 53-60.

   26. Masayuki Satake, Kimberly Cornelio, Shinya Hanashima, Raymond Malabed, Michio Murata, Nobuaki Matsumori, Hui-Ping Zhang, Fumiaki Hayashi, Shoko Mori, Jong Souk Kim, Chang-Hoon Kim, Jong Soo Lee. Structures of the Largest Amphidinol Homologues from the Dinoflagellate Amphidinium carterae and Structure-Activity Relationships. J. Nat. Prod. 2017, 80, 2883-2888.

   27. Raymond Malabed, Shinya Hanashima*, Michio Murata*, Kaori Sakurai. Sterol-Recognition Ability and Membrane-Disrupting Activity of Ornithogalum Saponin OSW-1 and Usual 3-O-Glycosyl Saponins. BBA Biomembrane, 2017, 1859, 2516-2525.

   28. Masamichi Nagae, Sushil K. Mishra, Shinya Hanashima, Hiroaki Tateno, Yoshiki Yamaguchi*. Distinct Roles for Each N-glycan Branch Interacting with Mannose-Binding Type Jacalin-Related Lectins Orysata and Calsepa. Glycobiology 2017, 27, 1120-1133.

   29. Nakane T, Hanashima S, Suzuki M, Saiki H, Hayashi T, Kakinouchi K, Sugiyama S, Kawatake S, Matsuoka S, Matsumori N, Nango E, Kobayashi J, Shimamura T, Kimura K, Mori C, Kunishima N, Sugahara M, Takakyu Y, Inoue S, Masuda T, Hosaka T, Tono K, Joti Y, Kameshima T, Hatsui T, Yabashi M, Inoue T, Nureki O, Iwata S, Murata M, Mizohata E, Membrane Protein Structure Determination by SAD, SIR, or SIRAS Phasing in Serial Femtosecond Crystallography Using an Iododetergent. Proc. Natl. Acad. Sci. 2016, 113, 13039-13044.

   30. Kimberly Cornelio, Rafael Atillo Espiritua, Yasuto Todokoro, Shinya Hanashima, Masanao Kinoshita, Nobuaki Matsumori, Michio Murata, Shinichi Nishimura, Hideaki Kakeya, Minoru Yoshida, Shigeki Matsunaga. Sterol-Dependent Membrane Association of the Marine Sponge-Derived Bicyclic Peptide Theonellamide A as Examined by 1H NMR. Bioorg. Med. Chem. 2016, 24, 5235-5242.

   31. Md. Imran Hossain, Shinya Hanashima*, Takuto Nomura, Sébastien Lethu, Hiroshi Tsuchikawa, Michio Murata*, Hiroki Kusaka, Shunsuke Kita, Katsumi Maenaka. Synthesis and Th1-Immunostimulatory Activity of α-Galactosylceramide Analogues Bearing a Halogen-Containing or Selenium-Containing Acyl Chain. Bioorg. Med. Chem. 2016, 24, 3687-3695.

   32. Yasuo Nakagawa, Yuichi Umegawa, Naohiro Matsushita, Tomoya Yamamoto, Hiroshi Tsuchikawa, Shinya Hanashima, Tohru Oishi, Nobuaki Matsumori*, and Michio Murata*. The Structure of the Bimolecular Complex between Amphotericin B and Ergosterol in Membranes Is Stabilized by Face-to-Face van der Waals Interaction with Their Rigid Cyclic Cores. Biochemistry 2016, 55, 3392-402. 

   33. Masamichi Nagae, Akemi Ikeda, Shinya Hanashima, Takumi Kojima, Naoki Matsumoto, Kazuo Yamamoto, Yoshiki Yamaguchi. Crystal structure of human dendritic cell inhibitory receptor (DCIR) C-type lectin domain reveals the binding mode with N-glycan. FEBS Lett. 2016, 590, 1280-1288.

   34. Masamichi Nagae, Mayumi Kanagawa, Kana Morita-Matsumoto, Shinya Hanashima, Yasuhiko Kizuka, Naoyuki Taniguchi, Yoshiki Yamaguchi. Atomic visualization of a flipped-back conformation of bisected glycans bound to specific lectins. Scientific Report 2016, 6, 22973.

   35. Tomoya Yamamoto, Yuichi Umegawa, Hiroshi Tsuchikawa, Nobuaki Matsumori, Shinya Hanashima, Michio Murata, Resul Haser, Bernard J. Rawlings, Patrick Caffrey. Role of polyol moiety of amphotericin B in ion channel formation and sterol selectivity in bilayer membrane. Bioorg. Med. Chem. 2015, 23, 5782-5788.

   36. Shinya Hanashima, Sebastian Götze, Yan Liu, Akemi Ikeda, Kyoko Kojima-Aikawa, Naoyuki Taniguchi, Daniel Varón Silva, Ten Feizi, Peter H. Seeberger, Yoshiki Yamaguchi. Defining the interaction of human soluble lectin ZG16p and mycobacterial phosphatidylinositol mannosides. ChemBioChem, 2015, 16, 1502-1511.

   37. Yasuo Nakagawa, Yuichi Umegawa, Kenichi Nonomura, Naohiro Matsushita, Tetsuro Takano, Hiroshi Tsuchikawa, Shinya Hanashima, Tohru Oishi, Nobuaki Matsumori, Michio Murata. Axial Hydrogen at C7 Position and Bumpy Tetracyclic Core Markedly Reduce Sterol’s Affinity to Amphotericin B in Membrane. Biochemistry 2015, 54, 303-312.

   38. Shinya Hanashima, Hiroaki Korekane, Naoyuki Taniguchi, Yoshiki Yamaguchi. Synthesis of N-glycan units for assessment of substrate structural requirements of N-acetylglucosaminyltransferase III. Bioorg. Med. Chem. Lett. 2014, 24, 4533-4537.

   39. Ambara R. Pradipta, Ayumi Tsutsui, Akihiro Ogura, Shinya Hanashima, Yoshiki Yamaguchi, Almira Kurbangalieva, Katsunori Tanaka. Microfluidic mixing of polyamine with acrolein enables the detection of the [4+4] polymerization of intermediary unsaturated imines: The properties of a cytotoxic 1,5-diazacyclooctane hydrogel. Synlett. 2014, 25, 2442-2446.

   40. Joonil Cho, Kyohei Sawaki, Shinya Hanashima, Yoshiki Yamaguchi, Motoo Shiro, Kazuhiko Saigo, Yasuhiro Ishida. Stabilization of β-peptide helices by direct attachment of trifluoromethyl groups to peptide backbones. Chem. Commun. 2014, 50, 9855-9858.

   41. Ayumi Tsutsui, Rie Imamaki, Shinobu Kitazume, Shinya Hanashima, Yoshiki Yamaguchi, Masato Kaneda, Shinya Oishi, Nobutaka Fujii, Almira Kurbangalieva, Naoyuki Taniguchi and Katsunori Tanaka. Polyamine modification by acrolein exclusively produces 1,5-diazacyclooctanes: a previously unrecognized mechanism for acrolein-mediated oxidative stress. Org. Biomol. Chem. 2014, 12, 5151-5157.

   42. Mayumi Kanagawa, Yan Liu, Shinya Hanashima, Akemi Ikeda, Wengang Chai, Yukiko Nakano, Kyoko Kojima-Aikawa, Ten Feizi, Yoshiki Yamaguchi. Structural Basis for Multiple Sugar Recognition of Jacalin-related Human ZG16p Lectin. J. Biol. Chem. 2014, 289, 16954-16965.

   43. Soh Yamamoto, Ganesh Prasad Subedi, Shinya Hanashima, Tadashi Satoh, Michiro Otaka, Hideki Wakui, Ken-ichi Sawada, Shin-ichi Yokota, Yoshiki Yamaguchi, Hiroshi Kubota, Hideaki Itoh. ATPase activity and ATP-dependent conformational change in the co-chaperone HOP. J. Biol. Chem. 2014, 289, 9880-9886.

   44. Masamichi Nagae, Keisuke Soga, Kana Morita-Matsumoto, Shinya Hanashima, Akemi Ikeda, Kazuo Yamamoto, Yoshiki Yamaguchi. Phytohemagglutinin from Phaseolus vulgaris (PHA-E) displays a novel glycan recognition mode using a common legume lectin fold. Glycobiology, 2014, 24, 368-378.

   45. Shinya Hanashima, Akemi Ikeda, Hiroshi Tanaka, Yoshiyuki Adachi, Naohito Ohno, Takashi Takahashi, Yoshiki Yamaguchi. NMR study of short β1,3-glucans provides insights into the structure and interaction with Dectin-1. Glycoconj. J. 2014, 31, 199-207.

   46. Masamichi Nagae, Kousuke Yamanaka, Shinya Hanashima, Akemi Ikeda, Kana Morita-Matsumoto, Tadashi Satoh, Naoki Matsumoto, Kazuo Yamamoto, and Yoshiki Yamaguchi. Recognition of bisecting N-acetylglucosamine: structural basis for asymmetric interaction with the mouse lectin dendritic cell inhibitory receptor 2. J. Biol. Chem. 2013, 288, 33598-33610.

   47. Masamichi Nagae, Akemi Ikeda, Masaya Hane, Shinya Hanashima, Ken Kitajima, Chihiro Sato, and Yoshiki Yamaguchi. Crystal structure of anti-polysialic acid antibody single chain Fv fragment complexed with octasialic acid: Insight into the binding preference for polysialic acid. J. Biol. Chem. 2013, 288, 33784-33796.

   48.  Hiroaki Korekane, Jong Yi Park, Akio Matsumoto, Kazuki Nakajima, Shinji Takamatsu, Kazuaki Ohtsubo, Yasuhide Miyamoto, Shinya Hanashima, Kenji Kanekiyo, Shinobu Kitazume, Yoshiki Yamaguchi, Ichiro Matsuo, and Naoyuki Taniguchi. Identification of ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3) as a regulator of N-acetylglucosaminyltransferase GnT-IX (GnT-Vb). J. Biol. Chem. 2013, 288, 27912-27926.

   49. Shinya Hanashima, Chihiro Sato, Hiroshi Tanaka, Takashi Takahashi, Ken Kitajima, Yoshiki Yamaguchi. NMR study into the mechanism of recognition of the degree of polymerization by oligo/polysialic acid antibodies. Bioorg. Med. Chem. 2013, 21, 6069-6076.

   50. Hideyuki Ihara, Shinya Hanashima, Hiroki Tsukamoto, Yoshiki Yamaguchi, Naoyuki Taniguchi, Yoshitake Ikeda. Difucosylation of chitooligosaccharides by eukaryote and prokaryote α1,6-fucosyltransferases. Biochim. Biophys. Acta- General Subjects, 2013, 1830, 4482-4490.

   51. Shinya Hanashima, Noriko Fujiwara, Kana Matsumoto, Noriyuki Iwasaki, Guo-qing Zheng, Hidetaka Torigoe, Keiichiro Suzuki, Naoyuki Taniguchi, Yoshiki Yamaguchi. Solution 17O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase. Chem. Commun. 2013, 49, 1449-1451.

   52.  Hiroshi Tanaka, Tetsuya Kawai, Yoshiyuki Adachi, Shinya Hanashima, Yoshiki Yamaguchi, Shoji Ohno, Takashi Takahashi. Synthesis of β(1,3) oligoglucans exhibiting a Dectin-1 binding affinity and their biological evaluation. Bioorg. Med. Chem. 2012, 20, 3898-3914.

   53. Ganesh P. Subedi, Tadashi Satoh, Akemi Ikeda, Shinya Hanashima, Yoko Fujita-Yamaguchi, Yoshiki Yamaguchi. Overproduction of anti-Tn antibody MLS128 single-chain Fv fragment in Escherichia coli cytoplasm using a novel pCold-PDI vector. Protein Expr. Purif. 2012, 82, 197-204.

   54. Ayano Matsumoto-Takasaki, Shinya Hanashima, Ami Aoki, Noriyuki Yuasa, Haruhiko Ogawa, Reiko Sato, Hiroko Kawakami, Mamoru Mizuno, Hiroshi Nakada, Yoshiki Yamaguchi, Yoko Fujita-Yamaguchi. Surface plasmon resonance and NMR analyses of anti Tn-antigen MLS128 monoclonal antibody binding to two or three consecutive Tn-antigen clusters. J. Biochem. 2012, 151 273-282.

   55. Shinya Hanashima, Koichi Kato, Yoshiki Yamaguchi. 13C-NMR quantification of proton exchange at LewisX hydroxyl groups in water. Chem. Commun. 2011, 47, 10800-10802.

   56. adashi Satoh, Dan Hu, Yang Chen, Shinya Hanashima, Kazuo Yamamoto, Yoshiki Yamaguchi. Structural basis for oligosaccharide recognition of misfolded glycoproteins by OS-9 in ER-associated degradation. Mol. Cell 2010, 40, 905-916.

   57. Hideyuki Ihara, Shinya Hanashima, Takahiro Okada, Ritsu Ito Yoshiki Yamaguchi, Naoyuki Taniguchi, Yoshitaka Ikeda. Fucosylation of chitooligosaccharides by human α1,6-fucosyltransferase requires a non-reducing terminal chitotriose unit as a minimal structure. Glycobiology 2010, 20, 1021-1033.

   58. Shinya Hanashima, Ken-ichi Sato, Yuko Naito, Hiromu Takematsu, Yasunori Kozutsumi, Yukishige Ito, Yoshiki Yamaguchi. Synthesis and binding analysis of unique AG2 pentasaccharide to human Siglec-2 using NMR techniques. Bioorg. Med. Chem. 2010, 18, 3720-3725.

   59.  Yoshiki Yamaguchi, Shinya Hanashima, Hirokazu Yagi, Yutaka Takahashi, Hiroaki Sasakawa, Eiji Kurimoto, Takeshi Iguchi, Shigeyuki Kon, Toshimitsu Uede, Koichi Kato. NMR characterization of intramolecular interaction of osteopontin, an intrinsically disordered protein with cryptic integrin-binding motifs. Biochem. Biophys. Res. Commun. 2010 393 487-491.

   60. Yoshiki Yamaguchi, Masami Masuda, Hiroaki Sasakawa, Takashi Nonaka, Shinya Hanashima, Shin-ichi Hisanaga, Koichi Kato, Masato Hasegawa. Characterization of inhibitor-bound α-synuclein dimer: Role of α-synuclein N-terminal region in dimerization and inhibitor binding. J. Mol. Biol. 2010, 395, 445-456.

   61. Shinya Hanashima, Yoshiki Yamaguchi, Yukishige Ito, Ken-ichi Sato. Synthesis of the starfish ganglioside AG-2 pentasaccharide. Tetrahedron Lett. 2009, 50, 6150-6153.

   62. Shinya Hanashima, Ken-ichi Sato, Yukishige Ito, Yoshiki Yamaguchi. Silylene/oxazolidinone double-locked sialic acid building blocks for the efficient sialylation reaction in dichloromethane. Eur. J. Org. Chem. 2009, 4215-4220.

   63. Shinya Hanashima, Daichi Ishikawa, Shoji Akai, Ken-ichi Sato. Synthesis of the starfish ganglioside LLG-3 tetrasaccharide. Carbohydr. Res. 2009, 344, 747-752.

   64. Shinya Hanashima, Taku Tomiya, Shoji Akai, Ken-ichi Sato. Sialylation Reactions Using N-Glycolylneuraminic Acid Phosphite Donor. Carbohydr. Res. 2009, 344, 959-965.

   65. Shinya Hanashima, Shoji Akai, Ken-ichi Sato. Thioester-assisted α-sialylation reaction. Tetrahedron Lett. 2008, 49, 5111-5114.

   66. Shinya Hanashima, Peter H. Seeberger. Total Synthesis of Sialylated Glycans Related to Avian and Human Influenza Virus Infection. Chem. Asian J. 2007, 2, 1447-1459.

   67. Shinya Hanashima, Bastien Castagner, Davide Esposito, Toshiki Nokami, Peter H. Seeberger. Synthesis of a sialic acid α(2-3) galactose building block and its use in a linear synthesis of sialyl Lewis X. Org. Lett. 2007, 9, 1777-1779.

   68. Hiroshi Iijima, Nobuyuki Kasai, Hiromu Chiku, Tomofumi Takeuchi, Kouji Kuramochi, Shinya Hanashima, Susumu Kobayashi, Fumio Sugawara, Kengo Sakaguchi, Hiromi Yohida, Yoshiyuki Mizushina. Structure-activity relationship of a glycolipid, sulfoquinovosyl diacylglycerol, with the DNA binding activity of p53. Int. J. Mol. Med. 2007, 19, 41-48.

   69. Shinya Hanashima, Kei-ichiro Inamori, Shino Manabe, Naoyuki Taniguchi, Yukishige Ito. Systematic synthesis of bisubstrate-type inhibitors of N-acetylglucosaminyltransferases. Chem. Eur. J. 2006, 12, 3499-3462 (with cover picture).

   70. Shinya Hanashima, Shino Manabe, Yukishige Ito. Divergent synthesis of sialylated glycan chains: combined use of polymer support, resin capture-release, and chemoenzymatic strategies. Angew. Chem. Int. Ed. 2005, 44, 4218-4224.

   71. Yoshiyuki Mizushina, Nobuyuki Kasai, Katsumi Miura, Shinya Hanashima, Masaharu Takemura, Hiromi Yoshida, Fumio Sugawara, Kengo Sakaguchi. Structural relationship of lithocholic acid derivatives binding to the N-terminal 8-kDa domain of DNA polymerase β.” Biochemistry 2004, 43, 10669-10677.

   72. Shinya Hanashima, Shino Manabe, Keiichiro Inamori, Naoyuki Taniguchi, Yukishige, Ito. Synthesis of bisubstrate type inhibitor for N-acetylglucosaminyltransferases. Angew. Chem. Int. Ed. 2004, 43, 5674-5677.

   73. Shinji Kamisuki, Shunya Takahashi, Yoshiyuki Mitzushina, Shinya Hanashima, Kouji Kuramochi, Susumu Kobayashi, Kengo Sakaguchi, Tadashi Nakata, Fumio Sugawara. Total Synthesis of Dehydroaltenusin. Tetrahedron 2004, 60, 5695-5700.

   74. Shinya Hanashima, Shino Manabe, Yukishige Ito. Polymer-Resin Hybrid Capture-Release Strategy for Rapid Oligosaccharide Construction. Synlett 2003, 979-982.

   75.  Chikako Murakami, Takayuki Yamazaki, Shinya Hanashima, Shunya Takahashi, Masaharu Takemura, Shonen Yoshida, Keisuke Ohta, Hiromi Yoshida, Fumio Sugawara, Kengo Sakaguchi, Yoshiyuki Mizushina. A novel DNA polymerase inhibitor and a potent apoptosis inducer: 2-mono-O-acyl-3-O-(α-D-sulfoquinovosyl)-glyceride with stearic acid. Biochim. Biophys. Acta 2003, 1645, 72-80.

   76. Madoka Watanabe, Shinya Hanashima, Yoshiyuki Mizushina, Hiromi Yoshida, Masahiko Oshige, Kengo Sakaguchi, Fumio Sugawara. Biotinylated lithocholic acids for affinity chromatography of mammalian DNA polymerases α and β. Bioorg. Med. Chem. Lett. 2002, 12, 287-290.

   77. Chikako Murakami, Takayuki Yamazaki, Shinya Hanashima, Shunya Takahashi, Keisuke Ohta, Hiromi Yoshida, Fumio Sugawara, Kengo Sakaguchi, Yoshiyuki Mizushina. Structure-function relationship of synthetic sulfoquinovosyl acylglycerols as mammalian DNA polymerase inhibitors. Archives of Biochemistry and Biophysics 2002, 403, 229-236.

   78.  Yoshitaka Matsumoto, Hiroeki Sahara, Tatsuya Fujita, Kumiko Shimozawa, Mika Takenouchi, Toshihiko Torigoe, Shinya Hanashima, Takayuki Yamazaki, Shunya Takahashi, Fumio Sugawara, Yoshiyuki Mizushina, Keisuke Ohta, Nobuaki Takahashi, Shinsei Gasa, Kouichi Jimbow, Kengo Sakaguchi, Noriyuki Sato. An immunosuppressive effect by synthetic sulfolipids deduced from sulfoquinovosyl diacylglycerols of sea urchin. TRANSPLANTATION 2002, 74, 261-267.

   79. Hiroeki Sahara, Shinya Hanashima, Takayuki Yamazaki, Shunya Takahashi, Fumio Sugawara, Seiji Ohtani, Masako Ishikawa, Yoshiyuki Mizushina, Keisuke Ohta, Kumiko Shimozawa, Shinsei Gasa, Kouichi Jimbow, Kengo Sakaguchi, Noriyuki Sato, Nobuaki Takahashi. Anti-tumor effect of chemically synthesized sulfolipids based on sea urchin’s natural sulfoquinovosylmonoacylglycerols. Japanese Journal of Cancer Research 2002, 93, 85-32.

   80. Shinya Hanashima, Yoshiyuki Mizushina, Takayuki Yamazaki, Keisuke Ohta, Syunya Takahashi, Hiroeki Sahara, Kengo Sakaguchi, Fumio Sugawara. Synthesis of sulfoquinovosylacylglycerols, inhibitors of eukaryotic DNA polymerase α and β. Bioorg. Med. Chem. 2001, 9, 367-376.

   81. Yoshiyuki Mizushina, Yuichi Sugiyama, Hiromi Yoshida, Shinya Hanashima, Takayuki Yamazaki, Shinji Kamisuki, Keisuke Ohta, Masaharu Takemura, Toyofumi Yamaguchi, Akio Matsukage, Shonen Yoshida, Mineo Saneyoshi, Fumio Sugawara, Kengo Sakaguchi. Galactosyldiacylglycerol, a mammalian DNA polymerase α-specific inhibitor from a sea alga, Petalonia bingbamiae. Biol. Pharm. Bull. 2001, 24, 982-987.

   82. Keisuke Ohta, Yoshiyuki Mizushina, Takayuki Yamazaki, Shinya Hanashima, Fumio Sugawara, Kengo Sakaguchi. Specific interaction between an oligosaccharide on the tumor cell surface and the novel antitumor agents, sulfoquinovosylacylglycerols. Biochem. Biophys. Res. Commun. 2001, 288, 893-900.

   83. Shinya Hanashima, Yoshiyuki Mizushina, Takayuki Yamazaki, Keisuke Ohta, Syunya Takahashi, Hiroyuki Koshino, Hiroeki Sahara, Kengo Sakaguchi, Fumio Sugawara. Structural determination of sulfoquinovosyldiacylglycerol by chiral syntheses. Tetrahedron Lett. 2000, 41, 4403-4407.

   84. Shinya Hanashima, Yoshiyuki Mizushina, Keisuke Ohta, Takayuki Yamazaki, Fumio Sugawara, Kengo Sakaguchi. Structure-activity relationship of a novel group of mammalian DNA polymerase inhibitors, synthetic sulfoquinovosylacylglycerols. Japanese Journal of Cancer Research 2000, 91, 1073-1083.

   85.  Keisuke Ohta, Shinya Hanashima, Yoshiyuki Mizushina, Takayuki Yamazaki, Mineo Saneyoshi, Fumio Sugawara, Kengo Sakaguchi. Studies on a novel DNA polymerase inhibitor group, synthetic sulfoquinovosylacylglycerols: inhibitory action on cell proliferation. Mutation Research 2000, 467, 139-152.

   86. Y. Matsumoto, H. Sahara, T. Fujita, S. Hanashima, S. Takahashi, F. Sugawara, Y. Mizushina, K. Ohta, N. Takahashi, K. Jimbow, K. Sakaguchi, N. Sato. A novel immunosuppressive agent, SQDG, derived from sea urchin. Transplantation Proceeding 2000, 32, 2051-2053.

REVIEWS

1.  Shinya Hanashima, Yo Yano, Michio Murata, Enantiomers of Phospholipids and Cholesterol: A Key to Decipher Lipid-Lipid Interplay in Membrane. Chirality 2020, 32, 282-298.

2.  Hanashima Shinya. NMR Study of Glycan Interactions Using Synthetic Oligosaccharides. Trends in Glycoscience and Glycotechnology, 2019, 31, SE67-SE68.

3. 金川真由美、花島慎弥、山口芳樹βグルカンの立体構造とタンパク質による認識 実験医学（羊土社）、Vol.31, No.10　(増刊） 2013

4. Shinya Hanashima. NMR Conformational Analysis of α(2-8)Oligo- and Polysialic Acids. Trends in Glycoscience and Glycotechnology, 2012, 24, 228-230.

5.  Shinya Hanashima. Recent progress about efficient sialylation reactions. Trends in Glycoscience and Glycotechnology, 2011, 23, 111-121.

6. Shinya Hanashima. Chemoselective Ligation of the Glycosylphosphatidylinositol-Anchor with the Target Protein: Toward Total Synthesis of the Glycosylphosphatidylinositol-Anchored Protein. Trends in Glycoscience and Glycotechnology 2010, 22, 308-310.

7. Shinya Hanashima. Homogeneous glycoprotein synthesis using bacterial oligosaccharyltransferase. Trends in Glycoscience and Glycotechnology 2009, 21, 302-304.

BOOKS

1. CSJカレントレビュー　『CSJカレントレビュー 生命機能に関わる分子の最新化学 』 杉本直己、秋吉一成、二木史朗　編著　　花島慎弥、村田道雄　生命分子の機能を超えるための合成化学　共立出版 2018年　8月　(共著)

2. NMR分光法　阿久津秀雄、嶋田一夫、鈴木栄一郎、西村善文　編著　講談社　花島慎弥　村田 道雄　2016年4月(共著)

3. 糖鎖の新機能開発応用ハンドブック　秋吉一成　監修　NTS出版　花島慎弥　大1章三節　シアル酸のグリコシル化反応　2015年4月(共著)

4. Shinya Hanashima, Yoshiki Yamaguchi, “Applications of NMR to Biomolecular Systems of Interactions: An Overview” Bio-Nanotechnology, A Revolution in Food, Biomedical and Health Sciences 2013, (eds D. Bagchi, M. Bagchi, H. Moriyama and F. Shahidi), Blackwell Publishing Ltd., Oxford　(共著)

5. Yukishige Ito, Shinya Hanashima, “Rapid Synthesis of Oligosaccharide; Resin Capture-Release Strategy” Experimental Glycoscience Glycochemistry, 2008, 210-216, (eds. Naoyuki Taniguchi, Akemi Suzuki, Yukishige Ito, Hisashi Narimatsu, Toshisuke Kawasaki, Sumihiro Hase, Springer) 　 (共著)