Dr Huei-Ru Lin
林惠茹
Laboratory of Medicine and Biotechnology
Tzu-Chi University
Title : Associate professor
EMAIL : rita0107@gms.tcu.edu.tw
Tel : 2346 (Office) / 2345 (Lab)
Research interest
Our group develops bioanalytical methods using mass spectrometry. The projects include:
Testing of narcotics
–The development of bioanalytical tests of amphetamine, ketamine, and opiates, the most commonly abused drugs in Taiwan. The tests will be applied in different samples, such as urine, plasma, saliva, and exhaled breath.
Aging
–Quantification of advanced glycation end-products (AGEs) in human plasma. We will evaluate the efficiency of derivatization for AGEs using LC-MS/MS.
Metabolomics
–Quantification of indole-3-acetic acid biosynthesis pathway in bacteria
–Identification of drug-resistant strains of bacteria using metabolomic strategy
Environment
–The development of analytical assays for determining endocrine-disruptor compounds and antibiotics in soil
Published articles
Lin HR, Shu HY, and Lin GH*. Biological roles of indole-3-acetic acid in Acinetobacter baumannii. 2018. Microbiological research.216:30-39 (SCI) [paper]
Kang MG, and Lin HR*. Systematic evaluation and validation of benzodiazepines confirmation assay using LC-MS/MS. 2018. Journal of Analytical Toxicology. (SCI)
Lin HR, Hu A, Chiang CW, Liao CC, and Chang KC*. Rapid and sensitive detection of carbapenemase activity in Acinetobacter baumannii using superficially porous liquid chromatography-tandem mass spectrometry. 2016. J Microbiol Immunol Infect. 49: 910-917. (SCI) [pubmed]
Lin GH, Chang CY, and Lin HR*. Systematic profiling of indole-3-acetic acid biosynthesis in bacteria using LC–MS/MS. 2015. Journal of Chromatography B. 988:53–58.(SCI) [pubmed]
Chang KC, Kuo HY, Tang CY, Chang CW, Lu CW, Liu CC, Lin HR, Chen KS, and Liou ML*. Transcriptome profiling in imipenem-selected Acinetobacter baumannii. 2014. BMC genomics. 15:815-827. (SCI) [pubmed]
Lin HR*,Liao CC, and Lin TC. Improved identification of multiple drugs of abuse and relative metabolites in urine samples using liquid chromatography/triple quadrupole mass spectrometry coupled with a library search. 2014. Rapid Communications in Mass Spectrometry. 28:2043-2053. (SCI) [pubmed]
Lin HR*, Choi KI, Lin TC, and Hu A. Simultaneous quantification of amphetamine, opiates, ketamine and relative metabolites in urine for confirmatory analysis by liquid chromatography tandem mass spectrometry. 2013. Journal of chromatography B. 929: 133-141. (SCI) [pubmed]
Lin HR*, Chen CC, Huang CL, Chen ST, and Lua AC. Simultaneous determination of opiates, methadone, buprenorphine and metabolites in human urine by superficially porous liquid chromatography tandem mass spectrometry. 2013. Journal of chromatography B. 925: 10-15. (SCI) [pubmed]
Lua A, Lin SL, Lin HR, and Lu AC*. Replacing Immunoassays for Mephedrone, Six Amphetamine Type Stimulants and Ketamines with Flow Injection Analysis Tandem Mass Spectrometry. 2012. J Anal Toxicol. 36: 575-581.(SCI) [pubmed]
Work experience
2009.02-present
Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Taiwan
2007.10-present
‧Assistant Research Fellow
‧Method development and supervision of technical training of staff
‧Set up the laboratory
‧Set up ISO/IEC17025 system
2007.02-2007.10
Academia Sinica, Taiwan
‧Postdoctoral Fellow
‧Advised by Prof. Aden Chang
‧Participated in the research project “Splicing factor structure reconstruction”
‧Skills: 100-L scale fermentation, dissection of yeast, protein purification, basic TEM operation, SPIDER software operation (protein complex structure reconstruction)
2006.05-2006.12
Swiss Federal Institute of Technology in
Participated in the research program “Serum biomarker search by proteomics”
‧ Advised by Prof. Ruedi Aebersold
‧ Skills: operation of LC-MS (Finnegan LTQ), MALDI-TOF-TOF (ABI 4800), OGE (Agilent, 3100 off-gel electrophoresis), LC-MALDI spotter (LC packing and ABI Tempo), glycopeptides capture method, iTRAQ-labeling assay
1999.07-2006.04
Technical staff, Center for Drug of Abuse Analysis, Tzu Chi University, Taiwan
‧ Advised by Prof. Ahai-Chang Lua
‧ Responsible for routine analysis, quality control, and development of analytical methods
‧ Skills: operation of GC-MS (Agilent) , LC-MS (Finnigan LCQ), EIA (enzyme immunoassay)
From left to right: Chia-Yi Wu, Jia-Yun Taso, Chao-Chuan Liao, Huei-Ru Lin, (rear: Jeng-Han Lin)，Miao-Kuo Kang，Chung-Yu Chang