O: Characterize how seasonal photoperiod modulates circadian system dynamics in Culex pipiens and Culexquinquefasciatus
KR1: Complete timeseries qRT-PCR for long-day condition, activity monitoring data aquisition, and IHC image analysis.
KR2: Finalize all figures for publication.
R3: Complete and revise manuscript draft for publication.
O: Epigenetic Profiling of Histone Acetylation Marks in Fat BodyTissues Across Diapause and Non-Diapause States in Culex pipiens
KR1: Conduct rigorous quantitative and statistical analyses of Western blot data from diapausing and non-diapausing fat body tissues across multiple time points (0, 10, 20, 30, and 40 days) to generate publication-ready figures for the histone acetylation marks H3K27ac.
KR2: Conduct dsiRNA-mediated knockdown of key H3K27me3-regulated candidate gene, fatty acid synthase (fas) in Culex pipiens to investigate their potential roles in diapause regulation.
KR3: Validate gene knockdown efficiency using RT-qPCR and perform NMR-based metabolic profiling to compare molecular responses between diapause and non-diapause females.
O: RT-qPCR validation of miRNA Sequencing of Culex pipiens ovaries and fat bodies in Diapause and Non-Diapause Mosquitoes
KR1: Optimization of SYBR Green RT-qPCR protocol for miRNA validation on key miRNAs that are being upregulated during diapause.
KR2: Optimization of the artificial blood feeding protocol for Ixodes scapularis ticks.
KR3: Begin drafting manuscript on differential expression of miRNA in ovary and fat body tissues between diapausing and non-diapausing Culex pipiens mosquitoes.