O: Characterize how seasonal photoperiod modulates circadian system dynamics in Culex pipiens and Culexquinquefasciatus
KR1: Complete timeseries qRT-PCR for long-day condition, activity monitoring data aquisition, and IHC image analysis.
KR2: Finalize all figures for publication.
R3: Complete and revise manuscript draft for publication.
O: Epigenetic Profiling of Histone Acetylation Marks in Fat BodyTissues Across Diapause and Non-Diapause States in Culex pipiens
KR1: Conduct rigorous quantitative and statistical analyses of Western blot data from diapausing and non-diapausing fat body tissues across multiple time points (0, 10, 20, 30, and 40 days) to generate publication-ready figures for the histone acetylation marks H3K27ac.
KR2: Identify candidate histone deacetylase (HDAC) genes in Culex pipiens through gene annotation and design qRT-PCR primer.
KR3: Validate RT-qPCR assays and perform preliminary expression profiling of candidate HDAC genes in diapause and non-diapause females.
O: miRNA Sequencing of Culex pipiens ovaries and fat bodies in Diapause and Non-Diapause Mosquitoes
KR1: Collect ovary and fat body tissues from diapause and non-diapause mosquitoes and complete RT-qPCR miRNA validation on key miRNAs that are being upregulated during diapause.
KR2: Receive Ixodes scapularis shipment and begin the troubleshooting and optimization of preliminary rearing and blood feeding protocols.
KR3: Begin drafting manuscript on differential expression of miRNA in ovary and fat body tissues between diapausing and non-diapausing Culex pipiens mosquitoes.