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The Polymenis Lab
  • Overview
  • Research
  • People
  • Lab Stuff
    • Protocols
      • DNA content
      • Yeast Protein Extracts
      • Colony PCR (E. coli)
      • Colony PCR (S. cerevisiae)
      • APEX proximity labeling reactions
      • TAP-IP for mRBPs
      • RNA immunoprecipitation
      • APEX-IP for LC-MS/MS
      • Plasmid Recovery from yeast cells
  • Contact
The Polymenis Lab
  • Overview
  • Research
  • People
  • Lab Stuff
    • Protocols
      • DNA content
      • Yeast Protein Extracts
      • Colony PCR (E. coli)
      • Colony PCR (S. cerevisiae)
      • APEX proximity labeling reactions
      • TAP-IP for mRBPs
      • RNA immunoprecipitation
      • APEX-IP for LC-MS/MS
      • Plasmid Recovery from yeast cells
  • Contact
  • More
    • Overview
    • Research
    • People
    • Lab Stuff
      • Protocols
        • DNA content
        • Yeast Protein Extracts
        • Colony PCR (E. coli)
        • Colony PCR (S. cerevisiae)
        • APEX proximity labeling reactions
        • TAP-IP for mRBPs
        • RNA immunoprecipitation
        • APEX-IP for LC-MS/MS
        • Plasmid Recovery from yeast cells
    • Contact
  • For yeast protocols from SGD, see here.

  • For DNA content analysis, see here.

  • For protein extracts (for immunoblotting), see here. 

  • For colony PCR of E.coli, see here.

  • For colony PCR of S. cerevisiae, see here.

  • For plasmid recovery from yeast cells, see here.

  • For APEX labeling, see here.

  • For IPs of V5-tagged proteins, see here.

  • For IPs of TAP-tagged proteins see here, as modified for mRBPs, followed by ddPCR.

  • For APEX-IPs for MS/MS, see here.

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