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Monitoring yeast ageing - whole lifespan [Movie Link]
It is achieved for the first time continuous and high-resolution microscopic imaging of the entire replicative lifespan of single yeast cells. Our microfluidic dissection platform features an optically pre-aligned single focal plane and an integrated array of soft elastomer-based micropads; together allowing for trapping of mother cells, removal of daughter cells, monitoring gradual changes in aging and unprecedented microscopic imaging of the whole aging process. (green: vacuole is visualized by Vph1p-GFP)
: PNAS 2012,109 (13) 4916-4920, Nature Protocols, 2013, 8, 1019-1027
Aging device protocol [Movie Link]
Cell polarization [Movie Link]
Dynamics of yeast polarization under in the microfluidic device in response to an α-factor gradient: The yeast cells polarize towards higher concentrations of mating pheromone (α-factor; red). The cells express quadruple-Venus fluorescent protein under the control of the mating specific FIG1 promoter (green).
: Integrative Biology, 2012, 4 (4) 381-390
Single cell trapping: Chromosome dynamics [Movie Link]
A programmable microfluidic static droplet array [Movie Link]
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