SeeDB Videos

Supplementary Videos associated with the Nature Neuroscience publication.


Video 1 [Download]
Adult Thy1-YFP-H mouse hemi-brain (P70) imaged using confocal microscopy (10x). Serial x-y images were taken at an interval of 10 μm. Depths (z) indicated in the movie are non-calibrated values. The real depths are 1.49× larger.


Video 2 [YouTube] [Download]
Volume rendering of adult Thy1-YFP-H mouse (P72) cleared with SeeDB37 and imaged using two-photon microscopy (25x, 14x1 tiles). Images were taken from dorsal side, and 14×1 blocks were tiled.


Video 3 [Download]
Serial horizontal optical sections of Thy1-YFP-H line (P72). Hemi-brain sample was cleared with SeeDB37 and imaged using two-photon microscopy (25x, 35x13 tiles). Images were taken from dorsal side every 500 μm.


Video 4 [Download]
Thy1-YFP-H mouse (P72) imaged using two-photon microscopy (25x). Serial x-y images were taken at an interval of 25 μm.


Video 5 [Download]
Volume rendering of serial optical sections shown in Video 2.


Video 6 [Download]
Volume rendering of adult Thy1-YFP-H line (P72) cleared with SeeDB37 and imaged using two-photon microscopy (25x, 14x1 tiles). Images were taken from medial side.


Video 7 [Download]
Serial optical sections of Thy1-YFP-H line (P72) hemi-brain imaged from medial face using two-photon microscopy (25x, 35x17 tiles). Images were taken from the medial side every 300 μm, and 35×17 blocks were tiled.


Video 8 [YouTube] [Download]
Thy1-YFP-H line (P21) cleared with SeeDB37 and imaged using two-photon microscopy (25x). Images were taken from the dorsal side, and serial x-y images are shown.


Video 9 [Download]
Volume rendering of serial optical sections shown in Video 8.


Video 10 [Download]
The entire olfactory bulb of a Thy1-YFP-G mouse (P21) imaged using two-photon microscopy (10x). Serial tiled x-y images were taken from the medial side.


Video 11 [Download]
Callosal axons imaged using two-photon microscopy (25x, 5x4 tiles). Layer II-III pyramidal neurons were labeled with EGFP using in utero electroporation. Serial x-y images were taken at an interval of 10 μm.


Video 12 [Download]
Topographic organization of corpus callosum imaged using two-photon microscopy (25x, 13x19 tiles). Anterior and posterior regions of the cerebral cortex layer II-III neurons were labeled with tdTomato (magenta) and EGFP (green), respectively, using in utero electroporation. Serial x-y images were taken at an interval of 15 μm.


Video 13 [Download]
Reconstruction of individual callosal axons sparsely labeled by Cre-loxP system. Two-photon tiled images (25x, 9x4 tiles) were analyzed using Neurolucida software.


Video 14 [YouTube] [Download]
Tracing lateral dendrites of sister mitral cells (serial confocal images, 10x, 2x1 tiles). Depths (z) in the movie are
non-calibrated values. The real depths are 1.49× larger. Green, OMP-GFP; magenta, Alexa-647
dextran.


Related SeeDB Videos
Video 15
Cerebral cortex and hippocampus of adult Thy1-YFP-H mouse brain.
Imaging area was 4mm x 5mm (8x10 tiles), 2mm thickness.
Image resolution of this video can be changed by clicking the setting tab.

Video 16
Adult Thy1-YFP-H mouse brain (cortex, hippocampus, and thalamus).
Imaging area was 1.2mm x 1.2mm x 3.3mm.
10x water immersion lens was used for 2P imaging.