Publications

4) NEW: SeeDB2 paper Meng-Tsen Ke, Yasuhiro Nakai, Satoshi Fujimoto, Rie Takayama, Shuhei Yoshida, Tomoya S. Kitajima, Makoto Sato, and Takeshi Imai.  "Super-Resolution Mapping of Neuronal Circuitry with an Index-Optimized Clearing Agent". Cell Reports 142718–2732 (2016)


   RIKEN press release (English)
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     >> SeeDB2 protocol


High-resolution imaging. Cerebral cortex of adult Thy1-YFP-H mouse brain cleared with SeeDB2S and imaged with Airyscan.
Imaging area was 50um x 50um x 100um.

3) Meng-Tsen Ke, Satoshi Fujimoto, Takeshi Imai. "Optical clearing using SeeDB". bio-protocol.org (2014)

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2) Meng-Tsen Ke, Takeshi Imai. Optical clearing of fixed brain samples using SeeDB. Current Protocols in Neuroscience 66:2.22.1-19 (2014)

1) Meng-Tsen Ke, Satoshi Fujimoto, Takeshi Imai. “SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction”. Nature Neuroscience 16, 1154–1161 (2013)  doi: 10.1038/nn.3447



   RIKEN press release (English)
   RIKEN press release (Japanese)
   JST press release (Japanese)
   RIKEN RESEARCH (English)
   RIKEN CDB news (Japanese)


Large-scale imaging. Cerebral cortex and hippocampus of adult Thy1-YFP-H mouse brain cleared with SeeDB.
Imaging area was 4mm x 5mm (8x10 tiles of two-photon images), 2mm thickness.
Image resolution of this video can be changed by clicking the setting tab.


new SeeDB Kit is now commercialized from Wako Laboratory Chemicals


Contact: 
Takeshi Imai (Principal Investigator)
email: imai@cdb.riken.jp
Laboratory for Sensory Circuit Formation
RIKEN Center for Developmental Biology (CDB)
 
Since 6/24/2013
last updated: 5/16/2017