4) NEW: SeeDB2 paper Meng-Tsen Ke, Yasuhiro Nakai, Satoshi Fujimoto, Rie Takayama, Shuhei Yoshida, Tomoya S. Kitajima, Makoto Sato, and Takeshi Imai.  "Super-Resolution Mapping of Neuronal Circuitry with an Index-Optimized Clearing Agent". Cell Reports 142718–2732 (2016)

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     >> SeeDB2 protocol

High-resolution imaging. Cerebral cortex of adult Thy1-YFP-H mouse brain cleared with SeeDB2S and imaged with Airyscan.
Imaging area was 50um x 50um x 100um.

3) Meng-Tsen Ke, Satoshi Fujimoto, Takeshi Imai. "Optical clearing using SeeDB". (2014)

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2) Meng-Tsen Ke, Takeshi Imai. Optical clearing of fixed brain samples using SeeDB. Current Protocols in Neuroscience 66:2.22.1-19 (2014)

1) Meng-Tsen Ke, Satoshi Fujimoto, Takeshi Imai. “SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction”. Nature Neuroscience 16, 1154–1161 (2013)  doi: 10.1038/nn.3447

   RIKEN press release (English)
   RIKEN press release (Japanese)
   JST press release (Japanese)
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Large-scale imaging. Cerebral cortex and hippocampus of adult Thy1-YFP-H mouse brain cleared with SeeDB.
Imaging area was 4mm x 5mm (8x10 tiles of two-photon images), 2mm thickness.
Image resolution of this video can be changed by clicking the setting tab.

new SeeDB Kit is now commercialized from Wako Laboratory Chemicals

Takeshi Imai (Principal Investigator)
Laboratory for Sensory Circuit Formation
RIKEN Center for Developmental Biology (CDB)
Since 6/24/2013
last updated: 5/16/2017