Publications

4) NEW: SeeDB2 paper Meng-Tsen Ke, Yasuhiro Nakai, Satoshi Fujimoto, Rie Takayama, Shuhei Yoshida, Tomoya S. Kitajima, Makoto Sato, and Takeshi Imai.  "Super-Resolution Mapping of Neuronal Circuitry with an Index-Optimized Clearing Agent". Cell Reports 142718–2732 (2016)


   RIKEN press release (English)
   RIKEN press release (Japanese)
   JST press release (Japanese)
   RIKEN CDB news (Japanese)




     >> SeeDB2 protocol


High-resolution imaging. Cerebral cortex of adult Thy1-YFP-H mouse brain cleared with SeeDB2S and imaged with Airyscan.
Imaging area was 50um x 50um x 100um.

3) Meng-Tsen Ke, Satoshi Fujimoto, Takeshi Imai. "Optical clearing using SeeDB". bio-protocol.org (2014)

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2) Meng-Tsen Ke, Takeshi Imai. Optical clearing of fixed brain samples using SeeDB. Current Protocols in Neuroscience 66:2.22.1-19 (2014)

1) Meng-Tsen Ke, Satoshi Fujimoto, Takeshi Imai. “SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction”. Nature Neuroscience 16, 1154–1161 (2013)  doi: 10.1038/nn.3447



   RIKEN press release (English)
   RIKEN press release (Japanese)
   JST press release (Japanese)
   RIKEN RESEARCH (English)
   RIKEN CDB news (Japanese)


Large-scale imaging. Cerebral cortex and hippocampus of adult Thy1-YFP-H mouse brain cleared with SeeDB.
Imaging area was 4mm x 5mm (8x10 tiles of two-photon images), 2mm thickness.
Image resolution of this video can be changed by clicking the setting tab.


new SeeDB2 Kit is now commercialized from Wako Laboratory Chemicals

SeeDB Kit is commercialized from Wako Laboratory Chemicals


Contact: 
Takeshi Imai (Principal Investigator)
email: t-imai[at]med.kyushu-u.ac.jp
Graduate School of Medical Sciences, Kyushu University
 
Since 6/24/2013
last updated: 1/22/2018