Publications

What's new?

  • Tetbow paper was accepted: Richi Sakaguchi, Marcus N. Leiwe, Takeshi Imai. "Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags". eLife (2018, in press)
  • SeeDB2 step-by-step protocol is out in Bio-protocol: [HTML version]
  • SeeDB and SeeDB2 have been commercialized from FUJIFILM Wako Pure Chemical: SeeDB Kit and SeeDB2 Kit.

SeeDB

3) Meng-Tsen Ke, Satoshi Fujimoto, Takeshi Imai. "Optical clearing using SeeDB". Bio-protocol (2014)

[HTML version] [PDF version]

2) Meng-Tsen Ke, Takeshi Imai. “Optical clearing of fixed brain samples using SeeDB”. Current Protocols in Neuroscience 66:2.22.1-19 (2014)

1) Meng-Tsen Ke, Satoshi Fujimoto, Takeshi Imai. “SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction”. Nature Neuroscience 16, 1154–1161 (2013) doi: 10.1038/nn.3447

[PubMed] [PDF] [Supplementary Information]

RIKEN press release (English)

RIKEN press release (Japanese)

JST press release (Japanese)

RIKEN RESEARCH (English)

RIKEN CDB news (Japanese)

>> SeeDB protocol


Movie: Large-scale imaging. Cerebral cortex and hippocampus of adult Thy1-YFP-H mouse brain cleared with SeeDB. Imaging area was 4mm x 5mm (8x10 tiles of two-photon images), 2mm thickness. Image resolution of this video can be changed by clicking the setting tab.

>> More SeeDB Videos

SeeDB2

5) NEW: Meng-Tsen Ke, Takeshi Imai. "Optical Clearing and Index Matching of Tissue Samples for High-resolution Fluorescence Imaging Using SeeDB2". Bio-protocol (2018)

[HTML version]

4) Meng-Tsen Ke, Yasuhiro Nakai, Satoshi Fujimoto, Rie Takayama, Shuhei Yoshida, Tomoya S. Kitajima, Makoto Sato, and Takeshi Imai. "Super-Resolution Mapping of Neuronal Circuitry with an Index-Optimized Clearing Agent". Cell Reports 14, 2718–2732 (2016)

[PDF (article + supplement)]

RIKEN press release (English)

RIKEN press release (Japanese)

JST press release (Japanese)

RIKEN CDB news (Japanese)

>> SeeDB2 protocol


Image (cover) and Movie: High-resolution imaging. Cerebral cortex of adult Thy1-YFP-H mouse brain cleared with SeeDB2S and imaged with Airyscan. Imaging area was 50um x 50um x 100um.

>> More SeeDB2 Videos

Tetbow

6) NEW: Richi Sakaguchi, Marcus N. Leiwe, Takeshi Imai. "Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags". eLife (2018)

>> Tetbow protocol

Contact:

Takeshi Imai (Principal Investigator)

email: t-imai[at]med.kyushu-u.ac.jp

Graduate School of Medical Sciences, Kyushu University

Imai lab website

Lab Twitter @TakeshiImaiLab


Since 6/24/2013

last updated: 10/30/2018

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