Publications

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SeeDB

3) Meng-Tsen Ke, Satoshi Fujimoto, Takeshi Imai. "Optical clearing using SeeDB". Bio-protocol (2014)

[HTML version] [PDF version]

2) Meng-Tsen Ke, Takeshi Imai. “Optical clearing of fixed brain samples using SeeDB”. Current Protocols in Neuroscience 66:2.22.1-19 (2014)

1) Meng-Tsen Ke, Satoshi Fujimoto, Takeshi Imai. “SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction”. Nature Neuroscience 16, 1154–1161 (2013) doi: 10.1038/nn.3447

[PubMed] [PDF] [Supplementary Information]

RIKEN press release (English)

RIKEN press release (Japanese)

JST press release (Japanese)

RIKEN RESEARCH (English)

RIKEN CDB news (Japanese)

>> SeeDB protocol


Movie: Large-scale imaging. Cerebral cortex and hippocampus of adult Thy1-YFP-H mouse brain cleared with SeeDB. Imaging area was 4mm x 5mm (8x10 tiles of two-photon images), 2mm thickness. Image resolution of this video can be changed by clicking the setting tab.

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SeeDB2

5) NEW: Meng-Tsen Ke, Takeshi Imai. "Optical Clearing and Index Matching of Tissue Samples for High-resolution Fluorescence Imaging Using SeeDB2". Bio-protocol (2018)

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4) Meng-Tsen Ke, Yasuhiro Nakai, Satoshi Fujimoto, Rie Takayama, Shuhei Yoshida, Tomoya S. Kitajima, Makoto Sato, and Takeshi Imai. "Super-Resolution Mapping of Neuronal Circuitry with an Index-Optimized Clearing Agent". Cell Reports 14, 2718–2732 (2016)

[PDF (article + supplement)]

RIKEN press release (English)

RIKEN press release (Japanese)

JST press release (Japanese)

RIKEN CDB news (Japanese)

>> SeeDB2 protocol


Image (cover) and Movie: High-resolution imaging. Cerebral cortex of adult Thy1-YFP-H mouse brain cleared with SeeDB2S and imaged with Airyscan. Imaging area was 50um x 50um x 100um.

>> More SeeDB2 Videos

Tetbow

6) NEW: Richi Sakaguchi, Marcus N. Leiwe, Takeshi Imai. "Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags". eLife (2018)

[SSBD Database] [GitHub] [Addgene] [YouTube (1)] [YouTube (2)] [YouTube (3)] [YouTube (4)]

Kyushu Univ press release (Japanese)

Kyushu Univ press release (English)

>> Tetbow protocol


Image: Mitral and tufted cells in the olfactory bulb labeled with Tetbow. These neurons were labeled by in utero electroporation of Tetbow plasmids at P12 and analyzed at P7. After clearing with SeeDB2, confocal images were taken with Leica SP8.

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Contact:

Takeshi Imai (Principal Investigator)

email: t-imai[at]med.kyushu-u.ac.jp

Graduate School of Medical Sciences, Kyushu University

Imai lab website

Lab Twitter @TakeshiImaiLab


Since 6/24/2013

last updated: 2/10/2019

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