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QDyeFinder preprint: Leiwe et al., Automated neuronal reconstruction with super-multicolour fluorescence imaging. bioRxiv
Post-hoc correction of chromatic aberrations in cleared samples. Leiwe MN, Fujimoto S, Imai T. Post hoc Correction of Chromatic Aberrations in Large-Scale Volumetric Images in Confocal Microscopy. Front Neuroanat (2021)
The Tetbow image is now in a science museum, Miraikan, in Tokyo. See the image.
Tetbow image was featured as The Most Stunning Neuroscience Image 2018 (by Forbes) and The Photo of the Year 2018 (The Scientist).
Addgene blog post on Tetbow was published.
Tetbow paper was published: Richi Sakaguchi, Marcus N. Leiwe, Takeshi Imai. "Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags". eLife (2018)
SeeDB2 step-by-step protocol is out in Bio-protocol: [HTML version]
SeeDB and SeeDB2 have been commercialized from FUJIFILM Wako Pure Chemical: SeeDB Kit and SeeDB2 Kit.
Video 17.wmvSeeDB
Movie: Large-scale imaging. Cerebral cortex and hippocampus of adult Thy1-YFP-H mouse brain cleared with SeeDB. Imaging area was 4mm x 5mm (8x10 tiles of two-photon images), 2mm thickness. Image resolution of this video can be changed by clicking the setting tab.
>> SeeDB Videos
SeeDB2
Image (cover) and Movie: High-resolution imaging. Cerebral cortex of adult Thy1-YFP-H mouse brain cleared with SeeDB2S and imaged with Airyscan. Imaging area was 50um x 50um x 100um.
Tetbow (up to 7 colors)
Image: Mitral and tufted cells in the olfactory bulb labeled with Tetbow. These neurons were labeled by in utero electroporation of Tetbow plasmids at P12 and analyzed at P7. After clearing with SeeDB2, confocal images were taken with Leica SP8.
QDyeFinder
Image: Automated identification and reconstruction of hundreads of neurons in the cerebral cortex. We used a combination of 7 fluorescent proteins and then performed unsupervised clustering of color information to reconstruct neurites based on the color hues.
Contact:
Takeshi Imai (Principal Investigator)
email: imai.takeshi.457[at]m.kyushu-u.ac.jp
Graduate School of Medical Sciences, Kyushu University
Lab Twitter @TakeshiImaiLab
Since 6/24/2013
last updated: 4/12/2024
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