Publications
Moore SJ, Hleba YB, Bischoff S, Bell D, Polizzi KM, Freemont PS, 2021, Refactoring of a synthetic raspberry ketone pathway with EcoFlex., Microb Cell Fact, Vol: 20
Di Blasi R, Marbiah M, Siciliano V, Polizzi K, Ceroni F, 2021, A call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation, Nature Communications, Vol: 12, ISSN: 2041-1723
Aw R, De Wachter C, Laukens B, De Rycke R, De Bruyne M, Bell D, Callewaert N, Polizzi KM, 2021, Knockout of RSN1, TVP18 or CSC1‐2 causes perturbation of Golgi cisternae in Pichia pastoris, Traffic, Vol: 22, Pages: 48-63, ISSN: 1398-9219
Heide C, Buldum G, Moya-Ramirez I, Ces O, Kontoravdi K, Polizzi K, 2021, Design, development and optimisation of a functional mammalian cell-free protein synthesis platform, Frontiers in Bioengineering and Biotechnology, Vol: 8, ISSN: 2296-4185
Shmool TA, Martin LK, Clarke CJ, Bui-Le L, Polizzi KM, Hallett JP, 2021, Exploring conformational preferences of proteins: ionic liquid effects on the energy landscape of avidin, CHEMICAL SCIENCE, Vol: 12, Pages: 196-209, ISSN: 2041-6520
Davis B, Backus K, Winter G, Chica R, Li D, Lee SY, He C, Weeks A, Overall C, Hagihara S, Thuronyi B, Kamat S, Chen L-L, Hurtado Guerrero R, Yao S, Mahal LK, Voigt C, Woo C, Strauss E, Kikuchi K, Dore T, Radford S, Li XD, Heo WD, Superti-Furga G, Deans T, Belousov V, Matthews M, Jackson C, Malek S, Waldmann H, Rising A, Jewett M, Stamou D, Parker E, Murakami M, Polizzi K, Hamachi I, Erb T, Joo C, Uesugi M, Prinjha R, Rechavi G, Solano R, Schulman B, David Y, Oslund R, 2021, Voices of chemical biology, Nature Chemical Biology, Vol: 17, Pages: 1-4, ISSN: 1552-4450
Keck FD, Polizzi K, 2021, Microbial interventions are an easier alternative to engineer higher organisms, Microbial Biotechnology, Vol: 14, Pages: 26-30, ISSN: 1751-7907
Moya-Ramirez I, Bouton C, Kontoravdi C, Polizzi K, 2020, High resolution biosensor to test the capping level and integrity of mRNAs, Nucleic Acids Research, Vol: 48, Pages: 1-11, ISSN: 0305-1048
Aw R, Spice AJ, Polizzi K, 2020, Methods for expression of recombinant proteins using a Pichia pastoris cell-free system, Current protocols in protein science, Vol: 102, ISSN: 1934-3655
Spice AJ, Aw R, Bracewell DG, Polizzi KM, 2020, Improving the reaction mix of a Pichia pastoris cell-free system using a design of experiments approach to minimise experimental effort, Synthetic and Systems Biotechnology, Vol: 5, Pages: 137-144, ISSN: 2405-805X
Gschwend FJ, Hennequin LM, Brandt-Talbot A, Bedoya-Lora F, Kelsall GH, Polizzi K, Fennell PS, Hallett JP, 2020, Towards an environmentally and economically sustainable biorefinery: heavy metal contaminated waste wood as a low-cost feedstock in a low-cost ionic liquid process, Green Chemistry, Vol: 22, Pages: 5032-5041, ISSN: 1463-9262
Arpino JAJ, Polizzi KM, 2020, A modular method for directing protein self-assembly, ACS Synthetic Biology, Vol: 9, Pages: 993-1002, ISSN: 2161-5063
Bui-Le L, Clarke CJ, Bröhl A, Brogan APS, Arpino JAJ, Polizzi KM, Hallett J, 2020, Revealing the complexity of ionic liquid-protein interactions through a multi-technique investigation, Communications Chemistry, Vol: 3, ISSN: 2399-3669
Moore SJ, Lai H-E, Kelwick RJR, Chee SM, Bell DJ, Polizzi KM, Freemont PS, 2020, Correction to EcoFlex: a multifunctional MoClo kit for E. coli synthetic biology., ACS Synthetic Biology, ISSN: 2161-5063
Riangrungroj P, Polizzi KM, 2020, BeQuIK (Biosensor Engineered Quorum Induced Killing): designer bacteria for destroying recalcitrant biofilms., Microbial Biotechnology, Vol: 13, Pages: 311-314, ISSN: 1751-7915
Spice AJ, Aw R, Bracewell DG, Polizzi KM., 2020, Synthesis and assembly of Hepatitis B virus-like particles in a Pichia pastoris cell-free system, Frontiers in Bioengineering and Biotechnology, Vol: 8, ISSN: 2296-4185
Riangrungroj P, Bever CS, Hammock BD, Polizzi KM, 2019, A label-free optical whole-cell Escherichia coli biosensor for the detection of pyrethroid insecticide exposure, SCIENTIFIC REPORTS, Vol: 9, ISSN: 2045-2322
Marques MPC, Boyd AS, Polizzi K, Szita N, 2019, Microfluidic devices towards personalized health and wellbeing, JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, Vol: 94, Pages: 2412-2415, ISSN: 0268-2575
Riangrungroj P, Polizzi KM, 2019, BeQuIK (Biosensor Engineered Quorum Induced Killing): designer bacteria for destroying recalcitrant biofilms., Microbial Biotechnology, ISSN: 1751-7915
Moya-Ramirez I, Kontoravdi K, Polizzi KM, 2019, Low-cost and user-friendly biosensor to test the integrity of mRNA molecules suitable for field applications, Biosensors and Bioelectronics, ISSN: 0956-5663
Aw R, Polizzi KM, 2019, Biosensor-assisted engineering of a high-yield Pichia pastoris cell-free protein synthesis platform, BIOTECHNOLOGY AND BIOENGINEERING, Vol: 116, Pages: 656-666, ISSN: 0006-3592
Kotidis P, Jedrzejewski P, Sou SN, Sellick C, Polizzi K, Del Val IJ, Kontoravdi C, 2019, Model-based optimization of antibody galactosylation in CHO cell culture., Biotechnol Bioeng
Kylilis N, Riangrungroj P, Lai H-E, Salema V, Angel Fernandez L, Stan G-BV, Freemont PS, Polizzi KM, 2019, Whole-Cell Biosensor with Tunable Limit of Detection Enables Low-Cost Agglutination Assays for Medical Diagnostic Applications, ACS SENSORS, Vol: 4, Pages: 370-378, ISSN: 2379-3694
Kylilis N, Riangrungroj P, Lai H-E, Salema V, Fernández LÁ, Stan G-B, Freemont PS, Polizzi KM, 2019, Whole-cell biosensor with tuneable limit of detection enables low-cost agglutination assays for medical diagnostic applications, ACS Sensors, ISSN: 2379-3694
Trantidou T, Dekker L, Polizzi K, Ces O, Elani Y, 2018, Functionalizing cell-mimetic giant vesicles with encapsulated bacterial biosensors, INTERFACE FOCUS, Vol: 8, ISSN: 2042-8898
Aw R, McKay PF, Shattock RJ, Polizzi KM., 2018, A systematic analysis of the expression of the anti-HIV VRC01 antibody in Pichia pastoris through signal peptide optimization., Protein Expr Purif, Vol: 149, Pages: 43-50
Kylilis N, Tuza ZA, Stan G-B, Polizzi KM, 2018, Tools for engineering coordinated system behaviour in synthetic microbial consortia, NATURE COMMUNICATIONS, Vol: 9, ISSN: 2041-1723
Moore SJ, MacDonald JT, Wienecke S, Ishwarbhai A, Tsipa A, Aw R, Kylilis N, Bell DJ, McClymont DW, Jensen K, Polizzi KM, Biedendieck R, Freemont PS, 2018, Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from nonmodel bacteria, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 115, Pages: E4340-E4349, ISSN: 0027-8424
Tomazou M, Barahona M, Polizzi KM, Stan G-B, 2018, Computational Re-design of Synthetic Genetic Oscillators for Independent Amplitude and Frequency Modulation, CELL SYSTEMS, Vol: 6, Pages: 508-+, ISSN: 2405-4712
Elani Y, Trantidou T, Wylie D, Dekker L, Polizzi K, Law RV, Ces O, 2018, Constructing vesicle-based artificial cells with embedded living cells as organelle-like modules, SCIENTIFIC REPORTS, Vol: 8, ISSN: 2045-2322
Jonas FRH, Royle KE, Aw R, Stan G-BV, Polizzi KM, 2018, Investigating the consequences of asymmetric endoplasmic reticulum inheritance in Saccharomyces cerevisiae under stress using a combination of single cell measurements and mathematical modelling, SYNTHETIC AND SYSTEMS BIOTECHNOLOGY, Vol: 3, Pages: 64-75, ISSN: 1389-0166
Sou SN, Lee K, Nayyar K, Polizzi KM, Sellick C, Kontoravdi C, 2018, Exploring cellular behavior under transient gene expression and its impact on mAb productivity and Fc-glycosylation, BIOTECHNOLOGY AND BIOENGINEERING, Vol: 115, Pages: 512-518, ISSN: 0006-3592
Heide C, Ces O, Polizzi KM, Kontoravdi C, 2018, Creating cell-free protein synthesis factories, PHARMACEUTICAL BIOPROCESSING, Vol: 6, Pages: 3-6, ISSN: 2048-9145
Anastasiadi M, Polizzi K, Lambert RJW, 2018, An improved model for the analysis of combined antimicrobials: a replacement for the Chou-Talalay combination index method, JOURNAL OF APPLIED MICROBIOLOGY, Vol: 124, Pages: 97-107, ISSN: 1364-5072
Lai H-E, Moore S, Polizzi K, Freemont P, 2018, EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology., Pages: 429-444
Kylilis N, Stan G-B, Polizzi K, 2017, Tools for engineering coordinated system behaviour in synthetic microbial consortia
Royle KE, Polizzi K, 2017, A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris, SCIENTIFIC REPORTS, Vol: 7, ISSN: 2045-2322
Ogonah OW, Polizzi KM, Bracewell DG, 2017, Cell free protein synthesis: a viable option for stratified medicines manufacturing?, CURRENT OPINION IN CHEMICAL ENGINEERING, Vol: 18, Pages: 77-83, ISSN: 2211-3398
Dekker L, Polizzi KM, 2017, Sense and sensitivity in bioprocessing - detecting cellular metabolites with biosensors, CURRENT OPINION IN CHEMICAL BIOLOGY, Vol: 40, Pages: 31-36, ISSN: 1367-5931
Sou SN, Jedrzejewski PM, Lee K, Sellick C, Polizzi KM, Kontoravdi C, 2017, Model-based investigation of intracellular processes determining antibody Fc-glycosylation under mild hypothermia, BIOTECHNOLOGY AND BIOENGINEERING, Vol: 114, Pages: 1570-1582, ISSN: 0006-3592
Goers L, Ainsworth C, Goey CH, Kontoravdi C, Freemont PS, Polizzi KM, 2017, Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production, BIOTECHNOLOGY AND BIOENGINEERING, Vol: 114, Pages: 1290-1300, ISSN: 0006-3592
Goers L, Ainsworth C, Goey CH, Kontoravdi C, Freemont PS, Polizzi KM, 2017, Cover Image, Volume 114, Number 6, June 2017.
Walker BJ, Stan G-BV, Polizzi KM, 2017, Intracellular delivery of biologic therapeutics by bacterial secretion systems, EXPERT REVIEWS IN MOLECULAR MEDICINE, Vol: 19, ISSN: 1462-3994
Moore SJ, Lai H-E, Needham H, Polizzi KM, Freemont PS, 2017, Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool, BIOTECHNOLOGY JOURNAL, Vol: 12, ISSN: 1860-6768
Aw R, McKay PF, Shattock RJ, Polizzi KM, 2017, Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris, AMB EXPRESS, Vol: 7, ISSN: 2191-0855
Moore SJ, Lai H-E, Kelwick RJR, Ghee SM, Bell DJ, Polizzi KM, Freemont PS, 2016, EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology, ACS SYNTHETIC BIOLOGY, Vol: 5, Pages: 1059-1069, ISSN: 2161-5063
Klymenko OV, Shah N, Kontoravdi C, Royle KE, Polizzi KM, 2016, Designing an Artificial Golgi Reactor to Achieve Targeted Glycosylation of Monoclonal Antibodies, AICHE JOURNAL, Vol: 62, Pages: 2959-2973, ISSN: 0001-1541
del Val IJ, Polizzi KM, Kontoravdi C, 2016, A theoretical estimate for nucleotide sugar demand towards Chinese Hamster Ovary cellular glycosylation, SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322
Polizzi KM, Kontoravdi C, 2016, Vaccine production, ISBN: 9781439803332
Aw R, Polizzi KM, 2016, Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris, MICROBIAL CELL FACTORIES, Vol: 15, ISSN: 1475-2859
Polizzi KM, Freemont PS, 2016, Synthetic biology biosensors for healthcare and industrial biotechnology applications
Sou SN, Sellick C, Lee K, Mason A, Kyriakopoulos S, Polizzi KM, Kontoravdi C, 2015, How Does Mild Hypothermia Affect Monoclonal Antibody Glycosylation?, BIOTECHNOLOGY AND BIOENGINEERING, Vol: 112, Pages: 1165-1176, ISSN: 0006-3592
Polizzi KM, Kontoravdi C, 2014, Genetically-encoded biosensors for monitoring cellular stress in bioprocessing, Current Opinion in Biotechnology, Vol: 31, Pages: 50-56, ISSN: 1879-0429
Goers L, Freemont P, Polizzi KM, 2014, Co-culture systems and technologies: taking synthetic biology to the next level, JOURNAL OF THE ROYAL SOCIETY INTERFACE, Vol: 11, ISSN: 1742-5689
Jedrzejewski PM, del Val IJ, Constantinou A, Dell A, Haslam SM, Polizzi KM, Kontoravdi C, 2014, Towards Controlling the Glycoform: A Model Framework Linking Extracellular Metabolites to Antibody Glycosylation, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol: 15, Pages: 4492-4522, ISSN: 1422-0067
Behjousiar A, Constantinou A, Polizzi KM, Kontoravdi C, 2014, FIBS-enabled Noninvasive Metabolic Profiling, Jove-Journal of Visualized Experiments, ISSN: 1940-087X
Aw R, Polizzi KM, 2013, Can too many copies spoil the broth?, MICROBIAL CELL FACTORIES, Vol: 12, ISSN: 1475-2859
del Val IJ, Kyriakopoulos S, Polizzi KM, Kontoravdi C, 2013, An optimized method for extraction and quantification of nucleotides and nucleotide sugars from mammalian cells, ANALYTICAL BIOCHEMISTRY, Vol: 443, Pages: 172-180, ISSN: 0003-2697
Kyriakopoulos S, Polizzi KM, Kontoravdi C, 2013, Comparative analysis of amino acid metabolism and transport in CHO variants with different levels of productivity, JOURNAL OF BIOTECHNOLOGY, Vol: 168, Pages: 543-551, ISSN: 0168-1656
Constantinou A, Polizzi KM, 2013, Opportunities for bioprocess monitoring using FRET biosensors, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 41, Pages: 1146-1151, ISSN: 0300-5127
Arpino JAJ, Hancock EJ, Anderson J, Barahona M, Stan G-BV, Papachristodoulou A, Polizzi K, 2013, Tuning the dials of Synthetic Biology, MICROBIOLOGY-SGM, Vol: 159, Pages: 1236-1253, ISSN: 1350-0872
Goers L, Kylilis N, Tomazou M, Wen KY, Freemont P, Polizzi K, 2013, Engineering Microbial Biosensors, MICROBIAL SYNTHETIC BIOLOGY, Editors: Harwood, Wipat, Publisher: ELSEVIER ACADEMIC PRESS INC, Pages: 119-156, ISBN: 978-0-12-417029-2
Polizzi KM, 2013, What is synthetic biology?, Methods Mol Biol, Vol: 1073, Pages: 3-6
Chen N, Koumpouras GC, Polizzi KM, Kontoravdi C, 2012, Genome-based kinetic modeling of cytosolic glucose metabolism in industrially relevant cell lines: Saccharomyces cerevisiae and Chinese hamster ovary cells, BIOPROCESS AND BIOSYSTEMS ENGINEERING, Vol: 35, Pages: 1023-1033, ISSN: 1615-7591
Polizzi KM, Isaacson RL, 2012, Protein construct optimization: data sharing strategy, PROTEIN & CELL, Vol: 3, Pages: 321-322, ISSN: 1674-800X
Sou SN, Ilieva KM, Polizzi KM, 2012, Binding of human BiP to the ER stress transducers IRE1 and PERK requires ATP, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol: 420, Pages: 473-478, ISSN: 0006-291X
Behjousiar A, Kontoravdi C, Polizzi KM, 2012, In Situ Monitoring of Intracellular Glucose and Glutamine in CHO Cell Culture, PLOS ONE, Vol: 7, ISSN: 1932-6203
Stefani IC, Wright D, Polizzi KM, Kontoravdi C, 2012, The Role of ER Stress-Induced Apoptosis in Neurodegeneration, CURRENT ALZHEIMER RESEARCH, Vol: 9, Pages: 373-387, ISSN: 1567-2050
Chen N, del Val LJ, Kyriakopoulos S, Polizzi KM, Kontoravdi C, 2012, Metabolic network reconstruction: advances in in silico interpretation of analytical information, CURRENT OPINION IN BIOTECHNOLOGY, Vol: 23, Pages: 77-82, ISSN: 0958-1669
Baldwin G, Bayer T, Dickinson R, Ellis T, Freemont PS, Kitney RI, Polizzi K, Stan GB., 2012, Synthetic Biology - A Primer, ISBN: 9781848168626
Szita N, Polizzi K, Jaccard N, Baganz F, 2010, Microfluidic approaches for systems and synthetic biology, CURRENT OPINION IN BIOTECHNOLOGY, Vol: 21, Pages: 517-523, ISSN: 0958-1669
Chaparro-Riggers JF, Loo BLW, Polizzi KM, Gibbs PR, Tang X-S, Nelson MJ, Bommarius AS, 2007, Revealing biases inherent in recombination protocols, BMC BIOTECHNOLOGY, Vol: 7, ISSN: 1472-6750
Chaparro-Riggers JF, Rogers TA, Vazquez-Figueroa E, Polizzi KM, Bommarius AS, 2007, Comparison of three enoate reductases and their potential use for biotransformations, ADVANCED SYNTHESIS & CATALYSIS, Vol: 349, Pages: 1521-1531, ISSN: 1615-4150
Polizzi KM, Bommarius AS, Broering JM, Chaparro-Riggers JF, 2007, Stability of biocatalysts, CURRENT OPINION IN CHEMICAL BIOLOGY, Vol: 11, Pages: 220-225, ISSN: 1367-5931
Chaparro-Riggers JF, Polizzi KM, Bommarius AS, 2007, Better library design: data-driven protein engineering., Biotechnol J, Vol: 2, Pages: 180-191
Polizzi KM, Moore DA, Bommarius AS, 2007, A short-chain dehydrogenase/reductase from Vibrio vulnificus with both blue fluorescence and oxidoreductase activity, CHEMICAL COMMUNICATIONS, Pages: 1843-1845, ISSN: 1359-7345
Bommarius AS, Broering JM, Chaparro-Riggers JF, Polizzi KM, 2006, High-throughput screening for enhanced protein stability, CURRENT OPINION IN BIOTECHNOLOGY, Vol: 17, Pages: 606-610, ISSN: 0958-1669
Polizzi KM, Parikh M, Spencer CU, Matsumura I, Lee JH, Realff MJ, Bommarius AS, 2006, Pooling for improved screening of combinatorial libraries for directed evolution, BIOTECHNOLOGY PROGRESS, Vol: 22, Pages: 961-967, ISSN: 8756-7938
Polizzi KM, Chaparro-Riggers JF, Vazquez-Figueroa E, Bommarius AS, 2006, Structure-guided consensus approach to create a more thermostable penicillin G acylase., Biotechnol J, Vol: 1, Pages: 531-536
Bommarus AS, Polizzi KM, 2006, Novel biocatalysts: Recent developments, CHEMICAL ENGINEERING SCIENCE, Vol: 61, Pages: 1004-1016, ISSN: 0009-2509
Polizzi KM, Spencer CU, Dubey A, Matsumura I, Lee JH, Realff MJ, Bommarius AS, 2005, Simulation modeling of pooling for combinatorial protein engineering, JOURNAL OF BIOMOLECULAR SCREENING, Vol: 10, Pages: 856-864, ISSN: 1087-0571
Polizzi KM, Spencer CU, Dubey A, Matsumura I, Lee JH, Realff MJ, Bommarius AS, 2005, Simulation modeling of pooling for combinatorial protein engineering., J Biomol Screen, Vol: 10, Pages: 856-864, ISSN: 1087-0571