Projects

TRUe: Monitoring tropical plant biodiversity from eDNA samples : exploring water, sol and air attributes (2022-2023) - Funded by Agropolis Fondation (France)

Project leader: AC Thuillet

Scientists involved from our team : AC Thuillet, C Mariac, JF Renno, N Scarcelli

Biodiversity inventorying is a key step to sustainable approaches regarding our environment. Traditional methods, based on species recognition and individual counts are precise, but remain slow and costly. The pace of climate change and anthropogenic pressures make it an emergency to be able to move up a gear in terms of biodiversity monitoring. Environmental DNA (eDNA) approaches coupled with metabarcoding offer a great opportunity to complement traditional methods and to accelarate biodiversity studies. However, very few studies have used this approach to target terrestrial plant biodiversity. In addition, most studies based on eDNA use water or soil samples. Air sampling seems appropriate to study plants, but very few examples of use of this type of sampling to reveal plant biodiversity exist in the literature. The objective of this proposal is to test whether the plant biodiversity may be revealed through eDNA approaches by comparing efficiency of water, soil and airborne sampling. Specific questions that will be adresse are: (i) may DNA from water, soil, and air reflect the actual biodiversity of a territory ? (ii) to which extend are this three different types of sampling complementary or redundant? (iii) is one of these sampling more efficient than another to reveal biodiversity? The proposal will rely on an experimental design performed within an arboretum in Peru. Our local partners from the IIAP have precisely studied this arboretum in which every single tree is referenced and mapped. The three sources of eDNA (water, soil and air) will be analysed through a metabarcoding approach. Results of this project would have sound repercution on plant biodiversity studies and in particuar woud find echos in agroforetry managment.